Application of caspase-3 inhibitor to prepare preparation for promoting nerve regeneration after cerebral stroke

Abstract

The invention belongs to the field of cytobiology, and relates to application of a caspase-3 inhibitor to prepare a preparation for promoting nerve regeneration after cerebral stroke. A cerebral stroke mouse model animal experiment result shows that in the cerebral stroke restoration stage, expression of caspase-3 in newborn neural progenitor cells of subventricular zone and dentate gyrus is obviously increased, in-vitro cultured and extracted neural progenitor cells show that caspase-3 restricts the self-renewal capability of neural progenitor cells but does not cause apoptosis of cells; caspase-3 regulates and controls nerve regeneration by reducing phosphorylation of Akt; and experiment result proves that the caspase-3 inhibitor inhibits activity of caspase-3 and is capable of substantially promoting propagation and migration of neural progenitor cells of subventricular zone, thereby promoting regeneration of neuron and nerve function recovery after cerebral stroke.

Description

technical field [0001] The invention belongs to the field of cell biology, and relates to the use of Caspase-3 inhibitors in promoting nerve regeneration after cerebral apoplexy, in particular to the use of Caspase-3 inhibitors in preparing preparations for promoting neural function remodeling after cerebral apoplexy. Background technique [0002] According to reports, stroke is one of the leading causes of death and disability worldwide. Studies have pointed out that tPA thrombolytic therapy has obvious curative effect on acute ischemic cerebral stroke; however, many stroke patients who fail to receive tPA thrombolytic therapy in time often leave serious sequelae, including hemiplegia, sensory loss, etc. , memory loss, etc. Although the medical community has been making continuous efforts, there is still no effective treatment for neurological impairment after stroke. [0003] Recent studies have shown that neural regeneration has always existed in the subventricular zone...

AI Technical Summary

Problems solved by technology

Recent studies have proved that caspase activation, especially caspase-3 activation, is related to many biological processes that do not cause cell death, such as dendrite reduction, synaptic inhibition, cell differentiation and compensatory proliferation and other biological processes; also studies have found that brain Abundant amounts of caspase-3 were detected in non-apoptotic astrocytes after stroke; however, the role of caspase-3 in neurogenesis after stroke has not been reported so far

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