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Method for preparing fermented strawberry juice with therapeutic efficacy

A production method and technology for strawberry juice, which are applied in the directions of microorganism-based methods, food preparation, and functions of food ingredients, etc., can solve the problems of unrelated breeding medium and fermentation medium, and low yield of target products, etc.

Inactive Publication Date: 2015-03-25
HANGZHOU YUANPEITE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0024] However, there are certain defects in the strategy of genome rearrangement at present, mainly because there is no relationship between the breeding medium and the fermentation medium, so that the yield of the target product of the selected strains during fermentation is much lower than expected

Method used

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  • Method for preparing fermented strawberry juice with therapeutic efficacy
  • Method for preparing fermented strawberry juice with therapeutic efficacy
  • Method for preparing fermented strawberry juice with therapeutic efficacy

Examples

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preparation example Construction

[0104] (h) The preparation of the strawberry juice includes: adding 200g of strawberries to 1000mL of water, and grinding them into pulp.

[0105] In the embodiment of the present invention, Bacillus natto 10261 and Bacillus natto 10263 adopt the prior art, and Bacillus natto 10261 comes from China Industrial Microorganism Culture Collection Center (CICC), address: 24 Jiuxianqiao Middle Road, Chaoyang District, Beijing No. 6 building; the storage date is 2002, and the strain maintenance number is 10261; Bacillus natto 10263 is from the China Industrial Microbiology Culture Collection Center (CICC), address: Courtyard 6, No. 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing Building No. 1; the storage date was 2002, and the strain maintenance number was 10263; Lactobacillus casei Shirota was from Yakult (Shanghai) Co., Ltd.; the mouse anti-PQQ IgG monoclonal antibody PQQ-mAb was a commercially available product, purchased from Jiaxing Yuanke Biotechnology Co., Ltd., the p...

Embodiment 1

[0107] The present invention prepares strawberry juice rich in NK and PQQ through the breeding of Bacillus natto and Lactobacillus casei and their co-fermentation, comprising the following steps:

[0108] (1) Breeding of Bacillus natto BN-NKPQQ-RWX-507 with high yield of NK and PQQ

[0109] Using the substrate-induced adaptive strategy, the high-yield NK and PQQ Bacillus natto BN-NKPQQ-RWX-507 was obtained through genome rearrangement and high-throughput screening technology. The obtained Bacillus natto 10261 and 10263 producing NK and PQQ were subjected to nitrosoguanidine mutagenesis respectively; then the offspring were mixed for mutagenesis, and cell fusion was carried out (fusogenic agent was polyethylene glycol 4000), subcultured, Realized genome shuffling, optimized and integrated the dominant mutations of all mutagenized progenies of Bacillus natto 10261 and 10623, and bred a high-yield NK and PQQ Natto with high yield, vigorous growth, stable genetics, and suitable as...

Embodiment 2

[0121] The present invention prepares strawberry juice rich in NK and PQQ through the breeding of Bacillus natto and Lactobacillus casei and their co-fermentation, comprising the following steps:

[0122] (1) Breeding of Bacillus natto BN-NKPQQ-RWX-507 with high yield of NK and PQQ

[0123] Using the substrate-induced adaptive strategy, the high-yield NK and PQQ Bacillus natto BN-NKPQQ-RWX-507 was obtained through genome rearrangement and high-throughput screening technology. The obtained Bacillus natto 10261 and 10263 producing NK and PQQ were respectively subjected to nitrosoguanidine mutagenesis; then the offspring were mixed for mutagenesis, and cytoplasmic fusion was carried out (fusogenic agent was polyethylene glycol 4000), subcultured, Realized genome shuffling, optimized and integrated the dominant mutations of all mutagenized progenies of Bacillus natto 10261 and 10623, and bred a high-yield NK and PQQ Natto with high yield, vigorous growth, stable genetics, and suit...

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Abstract

The invention discloses a method for preparing fermented strawberry juice with a therapeutic efficacy. The method comprises following steps: respectively carrying out nitrosoguanidine mutagenesis on bacillus natto 10261 and 10263 for producing nattokinase and pyrroloquinoline quinine, and then mixing mutation progenies of the bacillus natto 10261 and 10263; carrying out cell fusion subculture, and carrying out high throughput screening by adopting a substrate-induced adaptive strategy on the basis of a genome rearrangement technology to obtain the bacillus natto for highly producing the nattokinase and the pyrroloquinoline quinine; carrying out nitrosoguanidine mutagenesis on lactobacillus casei, and screening out lactobacillus casei caused by methionine nutritional defects; carrying out co-fermentation on the strawberry juice by adopting the screened bacillus natto and lactobacillus casei, so as to prepare the strawberry juice containing nattokinase and pyrroloquinoline quinine. According to the strawberry juice rich in nattokinase and pyrroloquinoline quinine prepared by the method, the pyrroloquinoline quinine content reaches 86-135ng / mL; and the bacillus natto activity is 410-912U / mL.

Description

technical field [0001] The invention relates to the field of microbial engineering and fermentation, in particular to a method for preparing fermented strawberry juice with dietotherapy effect. Background technique [0002] Natto is fermented from soybeans by Bacillus natto, and has multiple physiological functions: [0003] ①The effect of natto on the cardiovascular system: Nattokinase (Nattokinase, NK), a symbolic physiologically active substance in natto, is a protein capable of dissolving thrombus. It consists of 275 amino acid residues and has a molecular weight of about 27KD. It is non-toxic, It has no side effects and is a serine protease whose thrombolytic effect even exceeds that of the thrombolytic agent urokinase. In addition to its direct thrombolytic effect, nattokinase also has the ability to promote the production of plasminogen activator in venous endothelial cells, thereby indirectly expressing its thrombolytic activity. Linoleic acid in natto accounts for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/02A23L2/84A23L1/29C12N15/03C12N15/01C12R1/07C12R1/245A23L33/00
CPCA23L2/02A23V2002/00A23V2400/125A23V2200/30
Inventor 阮子琦张哲滔莫凡邓惟吴渊
Owner HANGZHOU YUANPEITE BIOTECH
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