Composition for treating contact dermatitis comprising pellitorine as an active ingredient

A contact dermatitis and composition technology, applied in the field of contact dermatitis improving compositions, can solve problems such as imperfection

Inactive Publication Date: 2015-03-25
CHUNG ANG UNIV IND ACADEMIC COOP FOUND +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As mentioned above, the development of natural medicinal materials for the treatment of contact dermatitis is becoming an important i

Method used

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  • Composition for treating contact dermatitis comprising pellitorine as an active ingredient
  • Composition for treating contact dermatitis comprising pellitorine as an active ingredient
  • Composition for treating contact dermatitis comprising pellitorine as an active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1. Isolation of pellitorine compound from Caulis japonica extract

[0078] The dried Caulis Caulis (10.2 kg) was pulverized, extracted with 80% acetone at room temperature for 3 times and filtered, and the extract was concentrated under reduced pressure to obtain 340 g of extract. After concentrating the extract under reduced pressure, use 70% MeOH-Hexane (MeOH-Hexane), 70% MeOH-Chloroform (MeOH-Chloroform) for solvent fractionation, and fractionate into 3 layers (hexane layer, chloroform layer, 70 % methanol layer). Among them, silica gel (Silica gel) column chromatography is used for the hexane layer, and the concentration of the solvent is increased according to hexane-ethyl acetate = 1:0 to 0:1. After performing TLC, it is fractionated into 17 layers. Among them, for Fr.9 DaiSogel ODS-B and medium pressure liquid chromatography (MPLC) (60%MeOH→100%MeOH, gradient system) column chromatography were carried out on the layer, and then fractionated into 6 laye...

Embodiment 2

[0079] Example 2. Identification of the structure of pellitorine compound

[0080] Determination of the pellitorine compound obtained in Example 1 1 H and 13 C-NMR spectrum, the results are as follows:

[0081] ① 1 H-NMR (600MHz, CDCl 3 )

[0082] :δ H 7.18(1H,dd,J=10.8,15.0Hz,H-3'),6.12(1H,dd,J=10.8,15.0Hz,H-4'),6.05(1H,dt,J=6.6,15.0Hz ,H-5'),5.90(1H,br s,N-H),5.81(1H,d,J=15.0Hz,H-2'),3.15(2H,t,J=6.6Hz,H-1), 2.13(2H,m,H-6'),1.80(1H,m,H-2'),1.41(2H,m,H-7'),1.29(4H,m,H-8',9') ,0.92(6H,d,J=6.6Hz,CH 3 -3,4),0.88(3H,t,J=7.2Hz,CH 3 -10')

[0083] ② 13 C-NMR (150MHz, CDCl 3 )

[0084] :δ c 166.5(C-1'), 143.0(C-5'), 141.1(C-3'), 128.2(C-4'), 121.8(C-2'), 46.9(C-1), 32.8(C -6'),31.3(C-8'),28.5(C-7'),28.5(C-2),22.4(C-9'),20.1(C-3,4),13.9(C-10 ')

[0085] The pellitorine compound obtained in Example 1 was determined to be (E,E)-N-(2-methylpropyl)2,4-decadienamide represented by the following Chemical Formula 1 from the above results.

[0086] [chemical formula 1]

...

Embodiment 3

[0088] Example 3. Evaluation of the effect of pellitorine compound on regulating immune cell activity

[0089] 3-1. Isolation of splenocytes

[0090] In order to perform the experiments described below, splenocytes need to be isolated from experimental animals. That is, 6-7 week-old Balb / C mice were used as experimental animals provided by Orient Bio (Seongnam, Korea) Co., Ltd. The experimental animals were kept at 22-24°C and the humidity was 50%, with sufficient feed and water. Spleens excised from Balb / C mice were sufficiently crushed with a mesh, and connective tissue and the like were removed with a cell strainer (SPL, Korea), followed by centrifugation to obtain splenocytes. 10 ml of ACK solvent was added to the obtained splenocytes, reacted at room temperature for 5 minutes, and after red blood cells were removed, the splenocytes were separated by centrifugation twice.

[0091] 3-2. Determination of the ability of pellitorine compounds to inhibit the proliferat...

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Abstract

The present invention relates to a composition including pellitorine as an active ingredient for alleviating contact dermatitis. A pellitorine compound which is an active ingredient of the composition according to the present invention is secreted in the early stage of various strong inflammatory diseases so as to control the secretion of IL-1beta which is cytokine promoting an inflammatory response and also control the proliferation of T cells and the secretion of IL-2, IL-4, or IFN-gamma which is cytokine secreted from T cells so that immunity can be regulated. Moreover, the compound exhibits activities in controlling the secretion of IL-1beta, inhibiting the decomposition cleavage of caspase-1, having no cytotoxicity, and decreasing skin thickness in contact dermatitis in an experiment using a contact dermatitis animal model as an animal model. Accordingly, it is expected that the composition of the present invention can be usefully used as a pharmaceutical composition, a cosmetic composition, or a functional food composition for preventing or treating inflammatory skin diseases like contact dermatitis.

Description

technical field [0001] The present invention relates to a composition for improving contact dermatitis containing pellitorine as an active ingredient. More specifically, it relates to a composition for improving contact dermatitis containing (E,E)-N-(2-methylpropyl)2,4-decadienamide isolated from Caulis japonica as an active ingredient . Background technique [0002] Piper kadsura (Piper kadsura) is the stem of pepper vine, which is an evergreen vine belonging to the family Piperaceae. It is a plant distributed in southern Korea, Japan, Taiwan and other places. The aliases of pepper vine are also called sea wind vine, big wind vine, rock pepper, etc. Since ancient times, it has the effects of expelling wind and dampness, relaxing tendons and collaterals, and regulating circulation. At the same time, it is well known that it is also a medicinal material that can treat numbness symptoms, joint pain, tendon spasm, bruises, asthma, chronic cough and other diseases caused by wi...

Claims

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Application Information

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IPC IPC(8): A61K31/16A61P17/00A61K8/42A61Q19/00A61Q19/10A61Q1/02A23L1/29A23L33/00
CPCA23L33/10A23V2002/00A61K8/42A61K31/16A61Q1/02A61Q19/00A61Q19/10A61K2300/00A23V2200/318A23V2250/30
Inventor 李民怨林锺淳徐成俊李廷秀方孝元李都翼崔永郁李增壎朱成洙李相根
Owner CHUNG ANG UNIV IND ACADEMIC COOP FOUND
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