Preparation, application and usage method for biocarbon-immobilized composite pollution-degrading bacterium particle

A technology of complex pollution and degrading bacteria, applied in the restoration of contaminated soil, etc., can solve the problems of failure and inability to fundamentally eliminate soil pollution, etc., achieve low cost, enhanced in-situ repair effect duration, and good effect

Active Publication Date: 2015-04-01
江苏诺丽慧农农业科技有限公司
View PDF7 Cites 35 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These technologies all utilize the adsorption function of biochar to pollutants. In fact, the adsorption of biochar to pollutants is essentially a kind of physical adsorption. The use of biochar in soil not only has the problem of failure after adsorption s

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation, application and usage method for biocarbon-immobilized composite pollution-degrading bacterium particle
  • Preparation, application and usage method for biocarbon-immobilized composite pollution-degrading bacterium particle
  • Preparation, application and usage method for biocarbon-immobilized composite pollution-degrading bacterium particle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The technological process of biochar immobilization compound pollution degrading bacteria repairing polycyclic aromatic hydrocarbon contaminated soil method in this implementation example is as follows figure 1 As shown, the specific steps of using rice husk biochar to treat PAH-contaminated soil are as follows:

[0035] Preparation of biochar: under the condition of 350°C, use rice husks to dry and pulverize under anaerobic conditions, use a carbonization furnace to pyrolyze for 4 hours to prepare biochar, grind, and pass through an 80-mesh sieve;

[0036] Selection of degrading microorganisms: the selected strain was Enterobacter cloacae strain CCTCC NO:M 2012240, and the strain used was a heavy metal-resistant polycyclic aromatic hydrocarbon degrading strain purchased from China Center for Type Culture Collection.

[0037] Preparation of biochar-immobilized microorganisms: add 20g PVA and 2g SA to 200mL distilled water and soak overnight, then stir at room temperatur...

Embodiment 2

[0051] The technological process of the biochar-immobilized microbial remediation method for PAH-contaminated soil in this implementation example is as follows: figure 1 As shown, the specific steps of using cow dung biochar to treat PAH-contaminated soil are as follows:

[0052] Preparation of biochar: At a temperature of 350°C, dry the pulverized material with cow dung under anaerobic conditions, use a carbonization furnace to pyrolyze for 4 hours to prepare biochar, grind it, and pass through an 80-mesh sieve;

[0053] Selection of degrading microorganisms: the selected strain was Enterobacter cloacae strain CCTCC NO:M 2012240, and the strain used was a heavy metal-resistant polycyclic aromatic hydrocarbon degrading strain purchased from China Center for Type Culture Collection.

[0054] Preparation of biochar-immobilized microorganisms: add 20g PVA and 2gSA to 200mL distilled water and soak overnight, then stir at room temperature for 15 minutes, heat to 95°C through a wat...

Embodiment 3

[0067] This embodiment is a variation example of embodiment 1, and the specific technical scheme is the same as that of embodiment 1, and the changes only lie in:

[0068] (1) In the preparation of biochar, the temperature is 300° C., the time is 5 hours, and the filter adopts a 100-mesh sieve;

[0069] (2) In biochar-immobilized microorganisms, the concentration of PVA is 5% (m / v), and the concentration of SA is 0.5% (m / v); the mass ratio of bacteria to carrier sol is 1:100; crosslinking agent CaCl in solution 2 The concentration is 20g / L;

[0070] (3) The weight ratio of biochar-immobilized compound pollution-degrading bacteria particles to polycyclic aromatic hydrocarbon compound-contaminated soil in coking plant is 0.5:100.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses preparation, application and a usage method for a biocarbon-immobilized composite pollution-degrading bacterium particle. The preparation method comprises: performing thermal cracking on air-dried crushed agricultural waste and animal manure, and grinding and sieving to obtain biocarbon; inoculating a strain of a composite pollution-degrading bacterium into a liquid medium for culturing, after the bacterium strain grows to the logarithmic phase, centrifuging and enriching the degrading bacterium, so as to obtain a composite pollution-degrading bacterium thallus; adding the composite pollution-degrading bacterium thallus into biocarbon, so as to obtain biocarbon adsorbing the composite pollution-degrading bacterium thallus; and adding the biocarbon adsorbing the composite pollution-degrading bacterium thallus into a carrier sol, so as to obtain a composite sol; and dropwise adding the composite sol into a crosslinking liquid to perform low-temperature crosslinking immobilization, and drying to obtain the biocarbon-immobilized composite pollution-degrading bacterium particle. The biocarbon-immobilized composite pollution-degrading bacterium particle has good bioactivity and chemical stability, is capable of effectively removing polycyclic aromatic hydrocarbons in composite polluted soil and accelerating recovery of soil functional microbes, and is low in cost, simple in preparation technology and obvious in restoration effect on polluted soil.

Description

technical field [0001] The invention relates to the field of biochar-immobilized microbial remediation pollution, in particular to the preparation, use and use method of biochar-immobilized composite pollution-degrading bacteria particles. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) is one of the typical persistent organic pollutants in soil, which has a strong "three-caused" effect and seriously threatens the ecological security of soil and human health. Therefore, the remediation of soil PAHs pollution has been It has become a hot issue of common concern at home and abroad. As a cost-effective means of removing organic pollution, microbial remediation has become the most potential treatment method at present, especially immobilized microbial technology provides a new way for the remediation of soil PAHs pollution. However, the current soil microbial remediation technology still has the disadvantages of low concen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): B09C1/10
Inventor 刘亮沈国清陈平付盼李蓝青
Owner 江苏诺丽慧农农业科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap