Large-scale production and fermentation culture medium of aschersonia placenta and fermentation cultivation method

A technology of Concepora platysma and a fermentation medium, which is applied to the large-scale production of fermentation medium and the field of fermentation culture of Conchosporium platysma, and can solve the problem of low sporulation efficiency and high cost of fermentation medium of Conchiomycete platysma , production and application obstacles and other problems, to achieve the effect of shortening the production cycle, good application prospects and low prices

Active Publication Date: 2015-04-08
HUAZHONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As an important biological control factor, many studies have used the fermentation product of Ascospora platinospores for biological control. However, due to the high cost of the fermentation medium and the long fermentation period of the current Ascospora platinospores, it usually takes 28 days to achieve the highest spore production. Compared with the 14-15 day fermentation period of the widely used biocontrol fungus Beauveria bassiana, the spore production efficiency of Ascospora platinum is low and the cost is high, which hinders its production and application

Method used

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  • Large-scale production and fermentation culture medium of aschersonia placenta and fermentation cultivation method
  • Large-scale production and fermentation culture medium of aschersonia placenta and fermentation cultivation method

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Embodiment 1

[0018] The screening of embodiment 1 bacterial strain

[0019] 1.1 Source of material

[0020] In my country's main citrus producing areas such as Hubei, Hunan, Jiangxi, Zhejiang, Sichuan, Chongqing, Guizhou, Fujian, Guangxi, Guangdong, etc., the carcasses of citrus whitefly infected by C.

[0021] Potato Dextrose Agar Medium (PDA): Boil 200g of potatoes to extract the juice, add 20g of glucose and 20g of agar powder, add water to 1000mL and boil. Afterwards, it was sterilized in a sterilizing pot (121°C, 30min).

[0022] Sterile operating conditions: All utensils and utensils must be sterilized in an autoclave (121°C, 30min), and inoculation and other operations are carried out in an ultra-clean workbench.

[0023] Culture conditions: culture in a constant temperature box with a temperature of 25°C and light (16L:8D). After the colony is formed, transfer it to a PDA slope, and then transfer it to a refrigerator at 4°C for storage.

[0024] 1.2 Isolation and identification ...

Embodiment 2

[0047] The invention relates to a fermenting medium of Septoria platinum, which is composed of the following components by weight percentage: 20% of peanut cake powder, 0.1% of glucose, 0.1% of tryptone and the balance of water.

[0048] The above-mentioned Ascospora platinis fermentation medium can be prepared according to conventional methods, and the sterilization conditions are: 121° C., pressure range 0.1 MPa-0.15 MPa, and time 30 minutes.

[0049] Fermentation and cultivation method of Ascospora platina: take the Ascospora platinospora YW3 strain with the preservation number of CCTCC NO: M2013448, inoculate it on PDA medium, and culture it in a constant temperature incubator at 25±1°C for 3 weeks. The aqueous solution of 0.05% Tween-80 collects spores and prepares 5×10 5 spore / ml suspension, the suspension is inoculated to the above-mentioned fermentation medium at last, the volume of the suspension is 10% of the fermentation medium volume, and placed in 27 ℃ for 15-16 d...

Embodiment 3

[0051] The invention relates to a fermenting medium of Septoria platinum, which is composed of the following ingredients in weight percentage: 25% of peanut cake powder, 0.05% of glucose, 0.05% of tryptone and the balance of water.

[0052] Fermentation and cultivation method of Ascospora platinoides: take the Ascospora platinospora WD3 strain with the preservation number CCTCC NO: M2013447, inoculate it on PDA medium, place it in a constant temperature incubator at 25±1°C and cultivate it for 2 weeks. 0.05% Tween-80 aqueous solution to collect spores and prepare 1 × 10 5 spore / ml suspension, the suspension is inoculated to the above-mentioned fermentation medium, the volume of the suspension is 15% of the volume of the fermentation medium, and placed at 27 ° C for 15-16 days after inoculation.

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Abstract

The invention discloses a large-scale production and fermentation cheap culture medium of aschersonia placenta. The large-scale production and fermentation culture medium comprises 15%-25% of groundnut meal, 0.05%-0.2% of glucose, 0.05%-0.2% of tryptone and the balanced water. The invention further discloses a fermentation cultivation method of the aschersonia placenta. The method includes the step that three high-virulence and high-spore-generation capacity aschersonia placenta bacterial strains including YW3, WD3 and ZJ5 are adopted. The aschersonia placenta is preserved in the China Center for Type Culture Collection, and the preservation numbers are CCTCC NO:M2013448, CCTCC NO:M2013447 and CCTCC NO:M2013446 respectively. By means of the fermentation cultivation method, the high-virulence and high-spore-generation capacity aschersonia placenta is obtained, and the large-scale production and fermentation culture medium has a good application prospect in prevention and treatment on aleyrodid class injurious insects.

Description

technical field [0001] The invention relates to a large-scale production fermentation medium and a large-scale production fermentation culture method of Aschersonia placenta. Background technique [0002] Ascospora platypsis is an important pathogenic fungus of whitefly pests. It has strong pathogenicity to whitefly pests, is non-toxic to humans, livestock and plants, does not kill natural enemies, and does not pollute the environment. It is currently a research and application at home and abroad. More pest biological control pathogenic fungi have been successfully used in the biological control of whitefly and scale insect pests in tea orchards. Investigations and studies have shown that Ascospora phyllospora can form an epidemic in whitefly populations and has a natural control effect. In recent years, the parasitism rate of this fungus on whiteflies in some citrus producing areas in southern my country is 30%-50%, and some Up to more than 90%. [0003] As an important bi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 张宏宇张为丽
Owner HUAZHONG AGRICULTURAL UNIVERSITY
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