Pseudomonas and microbial flocculant formed by fermenting pseudomonas

A microbial flocculant and microbial flocculation technology, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve problems that affect the normal growth of plants, affect human health, and corrode metals

Inactive Publication Date: 2015-04-22
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sludge produced by aluminum salts is widely used in agriculture, which leads to the increase of aluminum content in the soil and aluminum damage to plants, which affects the normal growth of plants and even dies; at the same time, th

Method used

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  • Pseudomonas and microbial flocculant formed by fermenting pseudomonas
  • Pseudomonas and microbial flocculant formed by fermenting pseudomonas
  • Pseudomonas and microbial flocculant formed by fermenting pseudomonas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Isolation and purification of Pseudomonas of the present invention

[0062] The Pseudomonas of the present invention is separated from the waste water of Jiangsu Hanyi Molding & Plastic Co., Ltd. by a plate marking method.

[0063] Isolation of flocculating bacteria: inoculate the obtained wastewater into the beef extract peptone liquid medium, and incubate at a constant temperature of 37°C for 24h. Dip a small amount of bacterial solution with an inoculation needle and draw a line on the beef extract peptone agar plate, and observe the growth of the colony regularly. Then, single colonies were picked by plate streaking method, and transferred to the slant of beef extract peptone agar for storage for later use.

[0064] Screening of Pseudomonas bacteria that produce microbial flocculants: screening by the flocculation effect of kaolin suspension.

[0065] (1) Primary screening: Inoculate each single colony picked out into a large amount of fermentation medium, and ...

Embodiment 2

[0079] Strain seed liquid culture: pick a small amount of bacteria from the slant of the beef extract peptone agar medium of Pseudomonas mendoza FL-1, inoculate it into the beef extract peptone liquid medium, and cultivate it at a constant temperature of 37°C for 24 hours. Seed liquid, wherein the bacterial concentration in the seed liquid is 2.3×10 6 individual / mL.

[0080] A large amount of liquid fermentation: inoculate the seed liquid into a large amount of fermentation medium in a ratio of 3% (250mL Erlenmeyer flask is filled into 100mL fermentation medium, and cultured on a shaking table at 160r / min at 30°C for 72h.

[0081] Centrifuge the fermented liquid obtained in a high-speed refrigerated centrifuge at 8000r / min for 20min at 4°C to obtain a supernatant, add twice the volume of absolute ethanol pre-cooled at 4°C to the supernatant, and let stand at 4°C Overnight, then centrifuge under the same centrifugation conditions, pour off the supernatant, dialyze the precipit...

Embodiment 3

[0083] This example provides the flocculation experiment of the microbial flocculant produced by Pseudomonas mendoza FL-1 on paint mist wastewater.

[0084] 1. Preparation of simulated paint mist wastewater

[0085] Take a daily plastic beverage bottle, add 100mL of tap water, adjust the pH to 12 with 1mol / L NaOH solution, then drip 6 drops of paint with a straw, shake vigorously, and the simulated paint mist wastewater is obtained.

[0086] 2. Preparation of microbial flocculant solution and auxiliary cation solution

[0087] As described in Example 2, the fermentation broth of the present invention cultured on a shaker at 160 r / min at 30° C. for 72 hours was prepared, which was directly used as a medicament for the flocculation test.

[0088] In addition, prepare 1% Na + 、K + , Mg 2+ , Ca 2+ , Fe 3+ 、Al 3+ NaCl, KCl, MgSO 4 , CaCl 2 , FeCl 3 、Al 2 (SO 4 ) 3 solution as an auxiliary cation solution.

[0089] 3. Experimental design and results

[0090] The first...

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Abstract

The invention provides a pseudomonas and an application of the pseudomonas. The preservation serial number of the pseudomonas is CGMCC No.9379, and the pseudomonas has the advantages of being high in growth speed, stability and flocculant yield, efficient and the like and can be used for producing a microbial flocculant in a fermenting mode, and especially be used for producing the microbial flocculant used for flocculating sprayed paint waste water in a fermenting mode.

Description

technical field [0001] The invention belongs to the field of microbial flocculants. Specifically, the present invention relates to a novel pseudomonas capable of fermenting and producing microbial flocculants, and the microbial flocculants produced by fermentation. Background technique [0002] The spray booth is a painting room dedicated to spraying liquid paint, and it is one of the key equipment in an industrial painting workshop. With the development of modern coating technology, the amount of spraying is increasing day by day, so the problem that spray paint cannot be applied to the workpiece and become "overspray" paint mist also appears in the spraying process. [0003] "Overspray" paint mist contains a large amount of volatile organic solvents that are not conducive to human health, seriously polluting the surrounding environment and air. The paint sticks to the ground and is difficult to clean, and it will also enter the circulating water to cause environmental po...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C02F3/34C02F1/52C12R1/38
CPCC02F1/52C02F3/34C12N1/20C12N1/205C12R2001/38
Inventor 董锐周冰倩
Owner YANCHENG INST OF TECH
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