Preparation method of edible oil peroxide value enzyme biosensor

Abstract

The object of the present invention is to aim at the above-mentioned deficiencies in the prior art, provide a kind of preparation method of the enzyme biosensor of peroxide value of edible oil, make it easy to industrialized production and disposable use, shorten sample processing and detection time, thereby Realize on-site, fast and accurate detection of samples. The steps are as follows: 1. Print a layer of chitosan film on the working electrode of the enzyme biosensor detection electrode by screen printing, print the mixed slurry of horseradish peroxidase, and dry at 50 ° C; 2. Print the substrate containing the enzyme again. 3. Mix the edible oil sample to be tested with the extract to form a reaction solution, and add it dropwise to 4. Detect the current between the working electrode and the counter electrode, compare it with the standard curve stored in the single-chip microcomputer, and convert it into the concentration of peroxide value.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a preparation method of an edible oil peroxide value enzyme biosensor. Background technique [0002] During the process of processing, storage, circulation, and use, oils and fats often undergo complex changes of rancidity and deterioration due to the effects of light, heat, and oxygen in the air. General oils, especially vegetable oils rich in unsaturated fatty acids, will oxidize and become rancid under the action of oxygen in the air, and produce intermediate products such as peroxides and hydroperoxides during the oxidation process, which are easily decomposed to produce volatile and non-volatile Natural fatty acids, aldehydes, ketones and alcohols, etc. These rancid products often have special odor and bitter taste, which greatly affect the sensory properties of oils and greatly reduce their nutritional value. The content of lipid peroxides in oil is represented by the m...

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Problems solved by technology

Published the invention document patent of detection peroxide value successively both at home and abroad, as Chinese patent (CN87106131.7) " peracetic acid measuring test paper and preparation method thereof " relate to the content of the peracetic acid in the water-soluble disinfectant of measuring with test paper; Japan The patent (53-30383) "Preparation method of test paper for the determination of peroxide in oil" uses soluble starch and potassium iodide as the main components, and both have the following defects: sensitive to light and oxygen, easy to fail; long reaction time, more than 5 minutes; Can only measure qualitatively and semi-quantitatively, cannot measure accurate values, etc.

The process of preparing the modified electrode by this method is quite complicated, the preparation time of the electrode is more than 2 hours, the prepared modified electrode has poor stability, and the modified electrode cannot be preserved for a long time. Widespread use

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