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Rapid propagation method for sagittaria sagittifolia tissue culture seedling and culture medium

A technology of tissue culture seedlings and subculture medium, which is applied in the field of rapid propagation method and culture medium of sagittarius tissue culture seedlings, can solve the problems of intermittent submerged bioreactors and reduce the cultivation process, and achieve the purpose of inhibiting the growth of bacteria, Ensure normal growth and reduce the effect of man-made pollution

Inactive Publication Date: 2015-04-29
广西壮族自治区农业科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The intermittent immersion bioreactor mainly uses the liquid medium to carry out intermittent immersion cultivation of plant tissue culture seedlings under the power of filtered air pressure. This cultivation method reduces the cultivation process, reduces labor costs, and reduces production costs. Based on With this advantage, the number of plant species that are being studied using batch immersion bioreactors at home and abroad is increasing, but there is no report on the use of batch immersion bioreactors for sagittarius tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take the tip of the stolon of Sagittarius sagittarius with a length of 1 cm, immerse it in water containing washing powder, wash it for 30 minutes, and after drying it, disinfect it with 75% ethanol for 30 seconds and 0.1% mercury liter for 10 minutes, and then rinse it with sterile water for 3 times; The explants of Sagittarius sagittarius were inoculated into solid medium and cultured for 3 weeks, wherein the formula of solid medium was: MS+6-BA2.0mg / L+NAA 0.5mg / L, sucrose concentration was 30g / L, agar 3.8g / L, and the pH value is 5.8; the culture conditions of the explants of Sagittarius sagittarius after detoxification in solid medium are: culture temperature 25°C, light 10h / d, dark 14h / d, light intensity 1000lx; The seedlings were inoculated into the intermittent submerged bioreactor, and the inoculation density was 8 plants / L; using the intermittent submerged culture method, the arrowhead explants were cultivated using liquid subculture medium, wherein the formula ...

Embodiment 2

[0025] Take the tip of the stolon of Sagittarius sagittarius with a length of 2cm, immerse it in water containing washing powder, wash it for 30 minutes, and after drying it, disinfect it with 75% ethanol for 30 seconds and 0.1% mercury liter for 10 minutes, and then rinse it with sterile water for 5 times; The explants of Sagittarius sagittarius were inoculated into solid medium and cultured for 3 weeks, wherein the formula of solid medium was: MS+6-BA 2.0mg / L+NAA 0.5mg / L, sucrose concentration was 30g / L, agar 3.8g / L, and the pH value is 6.2; the culture conditions of the explants of Sagittarius sagittarius after detoxification in solid medium are: culture temperature 28 ℃, light 10h, / d, dark 14h / d, light intensity 1500lx; The seedlings were inoculated into the intermittent submerged bioreactor, and the inoculation density was 15 plants / L; using the intermittent submerged culture method, the explants were cultivated using the liquid subculture medium, wherein the formula of ...

Embodiment 3

[0027] Take a 1.5cm-long stem tip of Arthia quinquefolium, immerse it in water containing washing powder, wash it for 30 minutes, and after drying, disinfect it with 75% ethanol for 30 seconds and 0.1% mercury liter for 10 minutes, and then rinse it with sterile water for 4 times; The explants of Sagittarius sagittarius were inoculated into solid medium and cultured for 3 weeks, wherein the formula of solid medium was: MS+6-BA 2.0mg / L+NAA 0.5mg / L, sucrose concentration was 30g / L, agar 3.8 g / L, and the pH value is 6.0; the culture conditions of the explants of Sagittarius sagittata after detoxification in solid medium are: culture temperature 26 ℃, light 10h / d, dark 14h / d, light intensity 1250lx; The seedlings were inoculated into the intermittent immersion bioreactor, and the inoculation density was 8 plants / L; using the intermittent immersion culture method, the arrowroot explants were cultivated using a liquid subculture medium, wherein the formula of the liquid subculture me...

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Abstract

The invention discloses a rapid propagation method for a sagittaria sagittifolia tissue culture seedling and a culture medium. The method comprises the following steps: cultivating a detoxified sagittaria sagittifolia explant for three weeks, and inoculating into a temporary immersion bioreactor; lastingly inputting filtering air by virtue of an intermittent immersion culture method; and cultivating the sagittaria sagittifolia tissue culture seedling with a liquid subculture medium without sugar. By semi-automatization, the manual operation is reduced; the pollution rate of the tissue culture seedling is reduced; the proliferation rate of the sagittaria sagittifolia tissue culture seedling is greatly increased; a stolon is adopted as an explant; collection and storage are facilitated; the sowing quantity of sagittaria sagittifolia corm is reduced; the production cost and the labor cost are reduced; a great amount of tissue culture seedlings can be obtained within a short period of time; and the rapid propagation method is short in production cycle, high in efficiency and suitable for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a rapid propagation method and culture medium of Sagittarius tissue cultured seedlings. Background technique [0002] Sagittaria trifolia (Sagittaria trifolia L.) belongs to the perennial aquatic herbaceous plant of Alismataceae Sagittaria. It is edible with its bulbs, and it is one of the main aquatic vegetables in the off-season for winter and spring vegetables. Arrowhead Arrowhead has high medicinal and health care value. It is bitter and sweet in nature and taste, slightly cold, non-toxic, and has the effects of cooling blood, stopping bleeding, detoxifying, and reducing swelling. The products are mainly exported to Southeast Asian countries. In recent years, the sales volume in domestic and foreign markets has increased greatly, and the supply of products is in short supply. Planting arrowroot has become one of the effective ways for farmers to increase their inco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 高美萍陈丽娟江文韦绍龙蔡炳华唐军桂杰何芳练杨柳董伟清闭志强欧昆鹏
Owner 广西壮族自治区农业科学院生物技术研究所
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