A method for detecting tartaric acid in edible fungus culture solution by using ion-exclusion chromatographic column
A technology of ion exclusion chromatography and tartaric acid, which is applied in the field of detection of tartaric acid in edible fungus culture solution by using ion exclusion chromatography column, can solve the problems of poor quantitative accuracy, different chromatographic conditions, cumbersome operation and other problems of thin layer chromatography, and achieve the goal of pre-sample The effect of simple processing, good repeatability and high precision
Active Publication Date: 2016-09-21
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology
The acid-base titration method can only measure the total acid content, and the operation is cumbersome; the gas chromatography method needs derivation steps, and the error is relatively large; the thin layer chromatography method has poor quantitative accuracy; while the high performance liquid chromatography method is easy to operate, with high accuracy and reproducibility. Well, it can quantify a variety of organic acids at the same time, and has been widely used in the determination of organic acids in various foods and natural products
However, different columns require different chromatographic conditions for the separation of different substances, and the qualitative and quantitative detection of organic acids in edible fungus culture liquid has not been reported yet
Method used
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Embodiment 1
[0028] Determine the content of tartaric acid in Flammulina velutipes G1, Flammulina velutipes F29, Pleurotus eryngii XH mycelia shake flask culture medium:
[0029] (1) Draw a standard curve
[0030] Accurately weigh 10mg of oxalic acid, citric acid, and tartaric acid standard products respectively, absorb 50 μL of formic acid and butyric acid standard products, place them in a 10 mL volumetric flask, and add ultrapure water to make up to volume as a stock solution. Dilute the stock solution to prepare mixed standards of different concentrations, inject samples for analysis, and draw a standard curve according to the peak area and organic acid content. The results are shown in Table 1.
[0031] Table 1 Standard curve, correlation coefficient and linear range
[0032] organic acid
regression equation
y=7.92e+0.03x-1.84e+0.04
0.9999
10-800μg / mL
y=9.52e+0.02x+3.39e+0....
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Abstract
The invention relates to a method for detecting tartaric acid in an edible fungus culture solution by utilizing an ion-exclusion chromatographic column. The method comprises the following steps: (1) preparing standard mother liquor, performing gradient dilution, and drawing a standard curve; (2) regulating the chromatographic conditions; (3) performing PDB flask-shaking culture to edible fungus, filtering and centrifuging and then taking supernatant for later use; and (4) according to the method of a standard substance, detecting the content of the tartaric acid in culture media and samples. The method is simple and has good precision and stability, the content of tartaric acid in the edible fungus fermenting liquor can be fast and accurately detected, and long-time and stable monitoring to the tartaric acid in the edible fungus culture solution under the corresponding culture mode or growth environment can be determined by the detection system.
Description
technical field [0001] The invention belongs to the field of determination of organic acids in edible fungus culture liquid, in particular to a method for detecting tartaric acid in edible fungus culture liquid by using an ion exclusion chromatographic column. Background technique [0002] Organic acids, as metabolites in the process of biological growth, will continue to change. During the growth of edible fungi, organic acids in the compost gradually accumulate, resulting in a drop in pH, which directly affects the metabolism of mycelial cells and the entire life activity. Therefore, understanding the organic acids in mycelia The types and changes are conducive to understanding its metabolic mechanism and better regulating the culture conditions to make it more suitable for the growth of mycelia. [0003] At present, the methods for analyzing organic acids mainly include: acid-base titration, gas chromatography, thin layer chromatography and high performance liquid chromat...
Claims
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IPC IPC(8): G01N30/02G01N30/46
Inventor 余昌霞曹晖王言汪虹陈明杰唐庆九
Owner SHANGHAI ACAD OF AGRI SCI