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Myocardial infarction rapid detection kit and preparation method thereof

A kit and rapid technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of delayed treatment, incompleteness, and single data of patients, and achieve economic and social benefits, simple operation, and high sensitivity Effect

Inactive Publication Date: 2015-04-29
GOLDBIOMARKERS DIAGNOSTICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the colloidal gold test strips and kits currently on the market are single-index test papers and kits. The data obtained are single and incomplete, and the detection sensitivity and specificity are relatively low. They cannot fully reflect the early condition of patients with myocardial infarction, and are prone to misdiagnosis or missed diagnosis.
MPO can warn of risk in the first 3 hours of myocardial infarction, but other inflammations can also lead to elevated MPO indicators
FABP3 can only express the situation 1-3 hours after the occurrence of myocardial infarction, and cannot warn before the occurrence of myocardial infarction
At present, most of the detection kits used in clinical practice mainly detect troponin and creatine kinase MB isoenzymes, and these old indicators appear after myocardial infarction, which can only show that myocardial infarction has occurred, and cannot warn of myocardial infarction
Although the electrocardiogram can also reflect the patient's condition, among the patients who have just developed chest pain, although some have already started the process of acute myocardial infarction, more than half of the electrocardiograms are normal, resulting in many patients dying due to delay in treatment
[0009] Looking at the existing products and literature reports, they all control a single indicator, and can only detect or warn of a certain stage of myocardial infarction, but cannot comprehensively and specifically warn the occurrence and development of myocardial infarction.
Since MPO and FABP3 are currently used clinically in different product forms, the production principles and operating procedures are different, and the compatibility is relatively poor. A reasonable and comprehensive judgment of the risk of myocardial infarction in patients with acute ACS

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  • Myocardial infarction rapid detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1. Preparation of MPO-FABP3-cTnI triple quantum dot kit

[0034] 1. Main material

[0035] MPO, FABP3 and cTnI standard products: National Institute of Inspection and Quarantine; MPO, FABP3 and cTnI specific antibodies and antigens: products of Beijing Shengqi Yanghuan Technology Co., Ltd.; quantum dots: products of Wuhan Jiayuan Company; nitrocellulose (NC) membrane: SARTORIUS ( Germany), CN140, product of Sartorius, Germany; bovine serum albumin (BSA), polyethylene glycol PEG20000, hydrolyzed casein: Sigma product; goat anti-mouse IgG antibody: Hangzhou Qitai product; other commonly used reagents are analytical Pure reagents.

[0036] The clinical samples were obtained by the company in relevant hospitals, with a total of 200 serum samples.

[0037] 2. Preparation method

[0038] (1) Quantum dot labeling: Dissolve quantum dot particles with a particle size of 5-10nm in PBS with a pH of 7.40.01M to make a solution with a concentration of 2umol, and add sp...

Embodiment 2

[0043] Embodiment 2. detection method

[0044] (1) Equilibrate the test reagents and samples to room temperature, take out the test paper card, and lay it flat;

[0045] (2) Accurately draw 10 μl of serum, add it to a clean centrifuge tube, dilute it 10 times with sample diluent (PBS or saline), and mix thoroughly;

[0046] (3) Use a pipette gun to draw 50-100ul of the diluted sample and add it to the sample hole, and after standing for 15-20 minutes, use a fluorescence quantitative reader to determine the quantitative result;

[0047] (3) When the instrument judges, after setting the relevant parameters of the instrument, put the test paper card into the chamber for detection, and the instrument will display the quantitative determination result of the sample concentration.

Embodiment 3

[0048] Example 3. Clinical sample detection

[0049] For the detection of 20 clinical value samples, when using the instrument to quantitatively determine the results, the average deviation of the 20 samples within the detection range is less than 15%, the maximum deviation is less than 20%, R2>0.97, and the consistency coefficient>0.88. The test results show that the prepared detection kit has good performance and is suitable for clinical detection.

[0050] 16 patients with clinically diagnosed myocardial infarction were tested with the kit of the present invention, and the measured data are shown in Table 1.

[0051] Table 1. Serum detection results of patients with myocardial infarction by MPO-FABP3-cTnI triple kit

[0052]

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Abstract

The invention discloses a myocardial infarction triple rapid detection kit and a preparation method for the myocardial infarction triple rapid detection kit. The kit is formed by taking novel heart markers, that is, human myeloperoxidase (MPO) and heart fatty acid binding protein (FABP3) as basis, and matching with any of myocardial infarction markers CRP (C reactive protein), cTnI and Lp-PLA2. The kit is characterized by being capable of warning myocardial infarction diseases much earlier compared with the existing gold-label kit, and capable of warning the risk of the whole process before and after the occurrence of myocardial infarction. The kit is composed of a test paper card and a colour comparison card or a colloidal gold quantitative reading meter, wherein an anti-MPO specific antibody, an anti-FABP3 specific antibody and a colloidal gold pad resisting to any of other myocardial infarction markers are labelled on the test paper card simultaneously; and novel myocardial infarction markers are detected simultaneously by a colloidal gold immunochromatography technology at the first time, and the kit has the characteristics of being capable of realizing early warning and whole-course monitoring, simple and convenient to operate, rapid, suitable for field detection, economical and practical, and the like.

Description

technical field [0001] The invention relates to a rapid detection kit for myocardial infarction and a preparation method thereof. Background technique [0002] Acute myocardial infarction (AMI) is a serious threat to human health and is one of the major diseases that cause death and disability worldwide. Rapid diagnosis of early acute myocardial infarction and timely treatment are the keys to reducing patient mortality. For patients with myocardial infarction without typical chest pain and insignificant ECG changes, it is difficult to accurately diagnose only by ECG, echocardiography, and cardiac MRI. Therefore, the detection of serum myocardial markers is a necessary basis for the diagnosis of AMI. [0003] Myocardial markers refer to a series of biochemical substances that can be measured in the blood. They are specific to the myocardium. When the myocardial tissue is damaged, they can be released into the circulating blood in large quantities. By detecting changes in blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/573G01N33/68
CPCG01N33/573G01N33/6803G01N33/577G01N33/68G01N33/533G01N2800/324G01N2333/916G01N33/54388G01N2333/4712G01N2333/70567G01N33/6893G01N2333/908G01N33/54313G01N2333/47G01N2333/4704
Inventor 胡文波李静
Owner GOLDBIOMARKERS DIAGNOSTICS
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