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A kind of Pseudomonas spinosa extract and its preparation method and application

A technology for the extract of Pseudomonas spp., applied in the field of Pseudomonas spp. extract and its preparation, can solve the problems of abnormal liver function, waste of resources, low yield, etc., and achieve energy saving and emission reduction and low cost , the effect of easy access to raw materials

Active Publication Date: 2019-04-05
陈乃宏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drugs that reduce uric acid production include xanthine oxidase (XOD) inhibitors, mainly allopurinol, but allopurinol causes adverse reactions such as allergy, liver and kidney damage, and bone marrow suppression, which limits its clinical application to a certain extent. application
In 2009, the FDA approved a xanthine oxidase / dehydrogenase selective inhibitor drug for the treatment of hyperuricemia: fexbuxostat, which has better safety than allopurinol for patients with moderate renal impairment, but also has liver Reports of adverse reactions such as functional abnormalities, gastrointestinal reactions, arthralgia and rashes
The yield of this method is low, the time is long, and resources are wasted

Method used

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  • A kind of Pseudomonas spinosa extract and its preparation method and application
  • A kind of Pseudomonas spinosa extract and its preparation method and application
  • A kind of Pseudomonas spinosa extract and its preparation method and application

Examples

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Embodiment 1

[0037] The preparation of the improved Pseudomonas spinosa extract of embodiment 1

[0038] 1000 g of Pseudomonas spinosa ants powder, 95% ethanol heating reflux extraction 3 times, 4 L each time, combined extracts, recovered ethanol under reduced pressure to obtain primary oil. Extract with petroleum ether (the boiling point of petroleum ether is 60°C-90°C), and recover the solvent to obtain 150g of petroleum ether. The petroleum ether part passes through the silica gel chromatography column, within 0 to 60h, the volume ratio of petroleum ether: ethyl acetate is eluted from the mobile phase gradient of 100%: 0% → 80%: 20%, and 121 fractions (500m L / part), the 11-22 fractions were recrystallized to obtain 20g of component I; the 28-36 fractions were subjected to repeated silica gel column chromatography and recrystallization to obtain 42g of component II, and the 40-48 fractions were repeatedly recrystallized to obtain components III 14g. Combine components I, II, and III a...

Embodiment 2

[0039] The preparation of the improved Pseudomonas spinosa extract of embodiment 2

[0040] 1000 g of Pseudomonas spinosa ants powder, 75% ethanol heating reflux extraction 3 times, 4 L each time, combined extracts, recovered ethanol under reduced pressure to obtain primary oil. Extract with petroleum ether (the boiling point of petroleum ether is 60°C-90°C), and recover the solvent to obtain 120g of petroleum ether. Petroleum ether parts passed through the silica gel column, within 0 to 55h, the volume ratio of petroleum ether: ethyl acetate was eluted from the mobile phase gradient of 100%:0% → 80%:20%, and 121 fractions (500m L / part), the 11-22 fractions were recrystallized to obtain 15g of component I; the 28-36 fractions were subjected to repeated silica gel column chromatography and recrystallization to obtain 30g of component II, and the 40-48 fractions were repeatedly recrystallized to obtain components III 10g. Combine components I, II, and III and purify with petr...

Embodiment 3

[0041] The preparation of the improved Pseudomonas spinosa extract of embodiment 3

[0042] 1000 g of Pseudomonas spinosa ants powder, 56% ethanol heating reflux extraction 3 times, 4 L each time, combined extracts, recovered ethanol under reduced pressure to obtain primary oil. Extract with petroleum ether (the boiling point of petroleum ether is 60°C-90°C), and recover the solvent to obtain 18g of petroleum ether. Petroleum ether parts passed through the silica gel column, within 0 to 65h, the volume ratio of petroleum ether: ethyl acetate was eluted from the mobile phase gradient of 100%:0% → 80%:20%, and 121 fractions (500m L / part), the 11-22 fractions were recrystallized to obtain 15g of component I; the 28-36 fractions were subjected to repeated silica gel column chromatography and recrystallization to obtain 10g of component II, and the 40-48 fractions were repeatedly recrystallized to obtain components III 5g. Combine components I, II, and III and purify with petrol...

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Abstract

The invention belongs to the technical field of natural extracts, and specifically relates to a polyrhachis vicina roger extract as well as a preparation method and an application thereof. The preparation method comprises the step of extracting the improved polyrhachis vicina roger extract by a process of ethanol extraction, petroleum ether extraction and silica gel chromatogram column separation. The preparation method disclosed by the invention is simple and practicable, convenient and easily available in raw material source, capable of being used for massively preparing the polyrhachis vicina roger extract, low in cost, less in pollution, beneficial to large-scale production under energy conservation and emission reduction conditions, and quite explicit in industrialization prospect. The improved extract can be used for preparing medicines for treating hypertension, hyperlipemia, atherosclerosis, adiposity, insulin resistance, nephropathy, inflammations, infectious diseases, tumour lysis syndrome, climacteric syndrome, organ transplantation complications, obstructive sleep apnea and primary sjogren syndrome caused by metabolic disorders of uric acid, and has a good prospect in both applications for foods and health products and applications for special diseases.

Description

technical field [0001] The invention belongs to the technical field of natural extracts, and in particular relates to an extract of Pseudomonas spp. and its preparation method and application. Background technique [0002] Uric acid is 2,6,8-purine trioxide, which is the end product of purine metabolism. Uric acid is a natural antioxidant that can remove 60% of free radicals in human blood. Uric acid reacts with peroxynitrite and superoxide to produce 1,3-di-carbamoylurea and allantoin, respectively. Most animals (including dogs, cats, mice and other mammals) can further decompose uric acid into allantoin due to the presence of uric acid decomposing enzyme (also known as urate oxidase oxidase) in the body. Allantoin is a non-toxic substance with good water solubility and will not cause damage due to deposition in tissues, so most animals do not have hyperuricemia and gout. [0003] Due to the lack of uric acid decomposing enzymes in the human body, uric acid, the end produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/64A61P19/08A61P9/12A61P3/06A61P9/10A61P3/04A61P5/50A61P13/12A61P29/00A61P31/00A61P35/00A61P15/12A61P37/06A61P11/00A61P17/00
Inventor 陈乃宏韦桂宁楚世峰
Owner 陈乃宏
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