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Transparent reproductive bacterial cellulose reproductive membrane as well as preparation method and application thereof

A technology of bacterial cellulose and regenerated membranes, applied in medical science, absorbent pads, prostheses, etc., can solve the problems of poor biomedical materials, poor light transmission and solubility, high crystallinity of bacterial cellulose and intermolecular forces and other problems, to achieve the effect of high density, high transmittance, and improved transparency

Active Publication Date: 2015-05-06
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the bacterial cellulose in the above-mentioned bioabsorbable fibers has high crystallinity and strong intermolecular forces, so its light transmittance and solubility are poor, and it is not effective for biomedical materials that require optical transparency.

Method used

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  • Transparent reproductive bacterial cellulose reproductive membrane as well as preparation method and application thereof
  • Transparent reproductive bacterial cellulose reproductive membrane as well as preparation method and application thereof
  • Transparent reproductive bacterial cellulose reproductive membrane as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A preparation method of a transparent degradable bacterial cellulose regenerated film, comprising the steps of:

[0078] (1) Preparation of bacterial cellulose

[0079] Preparation of culture medium: 0.1~0.4g / ml glucose, 0.03~0.06g / ml peptone, 0.02~0.03g / ml yeast powder, 0.01~0.02g / ml disodium hydrogen phosphate, 0.005~0.015g / ml magnesium sulfate, 0.005 ~0.01g / ml ammonium sulfate, 0.005~0.015ml / ml corn syrup extract, inoculate the bacterial cellulose production strain (Acetobacter xylinum, preservation number ATCC700178) into the culture medium at a ratio of 1:100 by medium volume solution, and then cultured statically for 3 to 7 days under clean conditions at 28°C to 32°C to obtain bacterial cellulose membranes.

[0080] (2) Purification of bacterial cellulose

[0081] The bacterial cellulose membrane obtained in the step (1) is washed repeatedly with a large amount of clear water to remove the culture medium and bacterial residues on the surface; Stir and soak for ...

Embodiment 2

[0097] In order to fully illustrate the beneficial effects of the present invention, the embodiments of the present invention also provide Example 2, the difference between Example 2 and Example 1. The step (3-2) of Example 2 adopts the coagulation bath regeneration method to prepare bacterial cellulose Regenerated film, other is identical with embodiment 1, and the step of embodiment 2 comprises:

[0098] (1) Preparation of bacterial cellulose

[0099] See Example 1.

[0100] (2) Purification of bacterial cellulose

[0101] See Example 1.

[0102] (3) Dissolution and film formation of bacterial cellulose

[0103] (3-1) Preparation of bacterial cellulose solution:

[0104] See Example 1.

[0105] (3-2) Prepare bacterial cellulose regenerated film by coagulation bath regeneration method: coat the prepared bacterial cellulose solution on a custom-made PTEF plate and scrape it into a film with a certain thickness (10 μm-1000 μm) with a custom-made mold, Then immerse the pla...

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Abstract

The invention provides a transparent reproductive bacterial cellulose reproductive membrane. The crystallinity of the transparent reproductive bacterial cellulose reproductive membrane is not more than 40%; the transparent reproductive bacterial cellulose reproductive membrane comprises the bacterial cellulose and a cellulase dispersed in the bacterial cellulose, wherein the mass ratio of the cellulase to the bacterial cellulose is 1:(10-100). The invention also provides a preparation method and application of the transparent reproductive bacterial cellulose reproductive membrane. The preparation method of the transparent reproductive bacterial cellulose reproductive membrane comprises the steps of to dissolving the bacterial cellulose by adopting an N, N-dimethylacetamide / lithium chloride mixed dissolving system, after reproducing the membrane, compounding the cellulase, thereby obtaining the degradable reproductive bacterial cellulose reproductive membrane with good transparency.

Description

technical field [0001] The invention relates to the technical field of functional materials, in particular to a transparent degradable bacterial cellulose regenerated membrane, a preparation method and application thereof. Background technique [0002] As a new type of biological material, bacterial cellulose has high crystallinity, high chemical purity, natural and fine three-dimensional network structure, good air permeability and water permeability, excellent water and water retention capacity, excellent mechanical properties and biocompatibility It has been widely used in the field of biomedical materials (such as artificial skin, artificial blood vessels, and artificial cartilage) due to its excellent performance. [0003] But at present, bacterial cellulose is mainly used as a body surface dressing or a permanent implant material in the body, which limits the application range of bacterial cellulose to a certain extent. This is mainly due to the lack of relevant enzym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/38A61L15/42A61L27/22A61L27/20A61L27/50
Inventor 朱勇军胡阳周新王金慧潘浩波
Owner SHENZHEN INST OF ADVANCED TECH
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