Acellular matrix of human heart valve tissue and preparation and application of acellular matrix

Abstract

The invention provides an acellular matrix of a human heart valve tissue and a preparation and an application of the acellular matrix. The preparation method of the acellular matrix of the human heart valve tissue is characterized by comprising the following specific steps: cutting the human heart valve tissue into sheets which are 50-100microns in thickness; carrying out acellular disposal on the obtained sheets to obtain the acellular matrix of a heart valve; and freeze-drying and sterilizing for later use. The obtained human tissue is firstly cut into the sheets and then is subjected to decellularizing operation; the decellularizing time is greatly shortened when the decellularizing thoroughness is ensured, so that a lot of time is shortened; and the possibility of commercialization in future is ensured. The acellular matrix provided by the invention also proves that the acellular matrix of the human heart valve tissue has the effects of promoting stem cell adhesion, proliferation and myocardial direction differentiation in in-vitro cell culture, and is transplanted to the surface of the myocardial infarction heart, so that the heart function can be effectively improved. Therefore, the acellular matrix can also be applied to stem cell therapy of the heart disease as a new in-vitro cell culture medium and a transplant.

Description

technical field [0001] The invention relates to the preparation of cardiac tissue decellularized matrix and its basic research and application in cell culture and tissue engineering, and belongs to the fields of biomedicine, tissue engineering, cell biology, stem cell biology and heart and great vessel surgery. Background technique [0002] Cardiovascular disease is a global health problem. About one-third of the world's people die from cardiovascular disease. Myocardial infarction and congestive heart failure are the main causes of death from cardiovascular diseases, and myocardial infarction is an important cause of congestive heart failure. At present, the treatment of myocardial infarction is mainly to restore the blood flow of myocardial tissue around the ischemic area. Traditional methods such as drug heart protection, coronary artery stent implantation and bypass surgery are generally adopted, but these treatment measures can only protect the remaining myocardium. I...

AI Technical Summary

Problems solved by technology

[0006] The invention creates a sheet-like decellularization method, which can solve the problem that the traditional substantive tissue decellularization method takes a long time and it is difficult to remove the cells in the middle part. After cutting into a membrane, the thickness of the membrane is close to that of the cells diameter, so most of the cells in the tissue can be exposed to the decellularization solution, it is easier to decellularize the cells, and the time-consuming can be greatly shortened

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