Compound microorganism microbial agent for promoting intestinal health of aquatic animals and preparation method of microbial agent

A technology of complex microbial agent and intestinal health, applied in the field of microbiology, can solve the problem of single inhibitory pathogenic bacteria, achieve the effect of reducing bait coefficient, promoting health and improving survival rate

Inactive Publication Date: 2015-05-06
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The beneficial effect of the invention is: the use of Bacillus pumilus has strong extracellular protease, lipase, amylase activity, has a wide range of inhibitory properties against Vibrio, plays a role in inhibiting the growth of vibrio, a pathogenic enteropathogen in prawns, and reducin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0049] And the specific operation steps of the preparation method of this composite microbial bacterial agent are as follows:

[0050] (1) Cultivation of Bacillus licheniformis strain FA08: take the Bacillus licheniformis strain FA08 ( Bacillus licheniformis ) and inoculate it into LB medium, and activate and cultivate it at 28-40°C, 150-200rpm for 5-10h; after activation, the seed liquid is obtained, and then inoculate the seed liquid into LB culture according to the inoculum amount of 1-10% base, and cultured at 28-40°C, 150-200rpm for 12-24h;

[0051] (2) Concentration and solidification of Bacillus licheniformis strain FA08: Centrifuge the cultured bacterial liquid at 8000-10000rpm for 5-10min after step (1), discard the supernatant to obtain a wet bacterial liquid; add pulverized bacterial liquid to the obtained wet bacterial liquid The fineness is 120-250 mesh diatomite or medical stone powder or zeolite powder 50g / L-2000g / L, and then dried at 30-40°C to obtain cured Ba...

Embodiment 1

[0059] Cultivation of strain FA08: Take strain FA08 and inoculate it into LB medium, and activate and culture it at 35°C and 170rpm for 5 hours; after activation, the seed liquid is obtained, and then inoculate the seed liquid into LB medium according to the inoculation amount of 8%. , and cultured at 28°C and 200rpm for 24 hours to obtain the strain FA08 bacterial liquid;

[0060] Concentration and solidification of bacterial strain FA08: put the strain FA08 bacterial liquid at 10000rpm and centrifuge for 8min, discard the supernatant to obtain the wet bacterial liquid; Add 1000g of diatomaceous earth to the humidified bacterial solution), and then dry it at 40°C to obtain the solidified Bacillus licheniformis strain FA08, which is ready for use;

[0061] Expansion of bacterial strain DS31: take bacterial strain DS31 ( Lactobacillus casei ) and inoculate it into MRS medium, and activate and culture it at 45°C and shaker at 180rpm for 10h; after activation, the seed liquid i...

Embodiment 2

[0066] Cultivation of strain FA08: take strain FA08 and inoculate it into LB medium, and activate and culture it at 28°C and 200rpm for 10 hours; after activation, the seed liquid is obtained, and then inoculate the seed liquid into LB medium according to the inoculum amount of 1%. , and cultivated at 40°C and 150rpm for 12 hours to obtain the strain FA08 bacterial liquid;

[0067] Concentration and solidification of bacterial strain FA08: place the strain FA08 bacterial liquid at 8000rpm and centrifuge for 10 minutes, discard the supernatant to obtain a wet bacterial liquid; add 500 g / L of wheat stone rice powder with a crushing fineness of 120-250 mesh to the obtained wet bacterial liquid (ie Add 500g of wheat stone rice powder to each liter of wet bacterial liquid), and then dry at 30°C to obtain cured Bacillus licheniformis strain FA08, which is ready for use;

[0068] Expansion of bacterial strain DS31: take bacterial strain DS31 ( Lactobacillus casei ) and inoculate it...

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Abstract

The invention provides a compound microorganism microbial agent for promoting intestinal health of aquatic animals. The compound microorganism microbial agent consists of the following components in percentage by mass: 5-25% of a cultivated, concentrated and immobilized strain FA08, 10-25% of an amplified, concentrated and immobilized strain DS31 and the balance of a carrier, wherein the carrier is diatomite, zeolite powder or medical stone powder, and the pulverization fineness of the diatomite, zeolite powder and medical stone powder is 120-250 meshes; meanwhile, the invention discloses a preparation method of the compound microorganism microbial agent. The compound microorganism microbial agent disclosed by the invention has an inhibiting effect on common pathogenic vibrio alginolyticus and aeromonas hydrophila in aquatic products; and through the synergistic effect of the two strains, the compound microorganism microbial agent can reduce the food coefficient of aquatic animals and promote the intestinal health of the aquatic animals and the utilization rate to feed more effectively, so that the survival rate of the aquatic animals is improved.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a compound microbial bacterial agent for promoting intestinal health of aquatic animals and a preparation method thereof. Background technique [0002] In recent years, food safety has attracted much attention, and healthy aquaculture of aquatic products is imperative. At present, in the process of aquaculture, most microbial preparations such as photosynthetic bacteria, Bacillus subtilis, and lactic acid bacteria are used to improve the breeding environment and reduce the occurrence of diseases. However, the occurrence of diseases in aquaculture animals is also related to the organisms of aquatic animals. Therefore, there have been many studies on the intestinal health of aquatic animals in recent years, and some of them use microorganisms to promote the intestinal health of aquatic animals. For example, the Chinese patent (patent number: 201210029204.9) applied by ...

Claims

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Application Information

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IPC IPC(8): C12N11/14A61K35/747A61K35/742A61P31/04A23K1/18A23K1/16C12R1/10C12R1/245
Inventor 徐长安唐旭刘源森林凌方卫东黄仕新
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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