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Medium capable of promoting germination of bletilla striata seeds and growth of seedlings and its formulation and preparation method

A culture medium and seed technology, applied in the field of plant tissue culture, can solve the problems of many endophytes, small reproduction coefficient, and no growth medium for Bletilla striata symbiotic bacteria, so as to avoid pollution and improve efficiency

Active Publication Date: 2016-08-17
HANGZHOU LEIWEI AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the above scheme solves the deficiencies of the prior art to a certain extent, the explants targeted by the medium are tubers of Bletilla striata, and compared with seed propagation, its reproduction coefficient is small and there are many endophytes. In addition, the medium Can not jointly promote Bletilla striata seed germination and seedling growth, there is no medium that can directly promote the growth of Bletilla striata symbiotic bacteria, nor can it inhibit the growth of miscellaneous bacteria

Method used

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  • Medium capable of promoting germination of bletilla striata seeds and growth of seedlings and its formulation and preparation method
  • Medium capable of promoting germination of bletilla striata seeds and growth of seedlings and its formulation and preparation method
  • Medium capable of promoting germination of bletilla striata seeds and growth of seedlings and its formulation and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation formula of the culture medium capable of promoting the germination of Bletilla striata seeds includes the following components: MS or 1 / 2MS, 0.1-2.0 mg / L NAA, 0.8-4.0 g / L activated carbon, 40-60 g / L mashed potatoes , 40-60g / L banana puree, 1-7g / L perilla stalk, 2-5g / L clematis, 3-10g / L Leulu, 2-9g / L notopterygium, 3-6g Viola chinensis / L, 2~5g / L dandelion leaves, 1~4g / L mountain bean root, 2~5g / L asparagus and 4~7g / L reed rhizome.

[0031] MS or 1 / 2MS was used as the basic medium, NAA promoted the germination of seeds, and banana puree and activated carbon were beneficial to the rooting of strong Bletilla striata seedlings. Perilla stalk, clematis, leaking reed, notopterygium, zihuadiding, light bamboo leaf, mountain bean root, asparagus and reed rhizome belong to the components of traditional Chinese medicine. Among them, perilla stalk, clematis, leaking reed, notopterygium, combined use can directly promote seed germination and seedling growth; Zihuadi...

Embodiment 2

[0045] The preparation formula of the culture medium that can promote the germination of bletilla striata seeds comprises the following components: the NAA of MS or 1 / 2MS, 0.1mg / L, the active carbon of 0.8g / L, the mashed potato of 40g / L, the banana of 40g / L Mud, 1g / L perilla stalk, 2g / L clematis, 3g / L Leulu, 2g / L notopterygium, 3g / L viola, 2g / L light bamboo leaves, 1g / L Shandou root, 2g / L asparagus and 4g / L reed rhizome.

[0046] As an improvement, this preparation formula also includes 1g / L Atractylodes atractylodes, 1g / L Artemisia argyi, 2g / L Angelica dahurica, 2g / L Sophora flavescens, 3g / L Cnidium chinensis, 2g / L white fresh bark, 3g / L basil, 2g / L cork and 3g / L onion.

[0047] Control experiment 2: The culture medium capable of promoting the germination of Bletilla striata seeds prepared by the preparation formula of this example was used as the culture medium of the experimental group; MS was also used as the culture medium of the control group. The experimental process ...

Embodiment 3

[0053] The preparation formula of the culture medium that can promote the germination of bletilla striata seeds comprises the following components: the NAA of MS or 1 / 2MS, 2.0mg / L, the active carbon of 4.0g / L, the mashed potato of 60g / L, the banana of 60g / L Mud, 7g / L perilla stalk, 5g / L clematis, 10g / L Leulu, 9g / L notopterygium, 6g / L zihuadiding, 5g / L light bamboo leaves, 4g / L Shandou root, 5g / L asparagus and 7g / L reed rhizome.

[0054] As an improvement, this preparation formula also includes 5g / L Atractylodes atractylodes, 3g / L Artemisia argyi, 3g / L Angelica dahurica, 5g / L Sophora flavescens, 4g / L Cnidium chinensis, 4g / L white fresh bark, 5g / L basil, 4g / L cork and 5g / L onion.

[0055] Control experiment 3: The culture medium capable of promoting the germination of Bletilla striata seeds prepared by the preparation formula of this example was used as the culture medium of the experimental group; MS was also used as the culture medium of the control group. The experimental p...

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a growth medium capable of promoting seed germination and seedling growth of bletilla striata, a formula of the growth medium and a preparation method. The preparation formula of the growth medium capable of promoting seed germination of bletilla striata comprises the following components: MS or 1 / 2 MS, 0.1-2.0 mg / L of NAA (naphthalene acetic acid), 0.8-4.0 g / L of activated carbon, 40-60 g / L of mashed potatoes, 40-60 g / L of mashed bananas, 1-7 g / L of perilla stem, 2-5 g / L of clematis chinensis, 3-10 g / L of uniflower swisscentaury root, 2-9 g / L of notopterygium incisum, 3-6 g / L of viola mandshurica, 2-5 g / L of lophatherum gracile, 1-4 g / L of vietnamese sophora root, 2-5 g / L of radix asparagi and 4-7 g / L of reed rhizome. The growth medium has the advantages as follows: MS or 1 / 2 MS is taken as the basic growth medium; NAA promotes seed germination; the mashed bananas and the activated carbon facilitate seedling strengthening and rooting; seed germination and seedling growth can be directly promoted by joint application of perilla stem, clematis chinensis, uniflower swisscentaury root and notopterygium incisum; growth of bletilla striata symbiotic bacteria can be promoted by viola mandshurica, lophatherum gracile, the vietnamese sophora root, radix asparagi and reed rhizome, and seed germination and seedling growth are promoted indirectly.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium capable of promoting the germination of bletilla striata seeds and the growth of seedlings, as well as its formula and preparation method. Background technique [0002] Bletilla striata is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae. It is the dry tuber of Bletilla striata, also known as ginger and purple orchid. Li Shizhen explained its name: its roots are white, connected and grow, so it is called Bletilla striata. Bletilla striata likes shade and is resistant to tides. It is mostly born in stone walls and moss layers. It is native to China and widely distributed in the provinces of the Yangtze River Basin. There is a small amount of cultivation throughout the country, and it is also distributed in Korea and Japan. Its flower color is gorgeous and colorful, and it is extraordinarily dignified and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 杜漾青郭铭心易浩君
Owner HANGZHOU LEIWEI AGRI DEV
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