Application of OsPSE1 gene in plant senescence regulation

A technology of plant senescence and transgenic plants, applied in the field of genetic engineering, can solve the problems of shortening the grain filling time of leaf functional period, limiting the improvement of crop yield and quality, and reducing dry matter accumulation, so as to achieve good application potential, delay plant senescence, Increase the effect of accumulation

Active Publication Date: 2015-05-13
SOUTH CHINA AGRI UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the period from heading to fruiting in rice, premature leaf senescence will shorten the leaf functional period and grain filling

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of OsPSE1 gene in plant senescence regulation
  • Application of OsPSE1 gene in plant senescence regulation
  • Application of OsPSE1 gene in plant senescence regulation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1 Rice senescence-related genes OsPSE1 / Ospse1 Construction of electronic physical map and screening of candidate genes

[0032] The present invention uses map-based cloning to clone OsPSE1 / Ospse1 gene( figure 1 ).

[0033] The inventors obtained rice plants with premature aging during tissue culture Ospse1 , The inventor crossed the plant with wild-type material CW312, and the hybrid F 1 The phenotypes of the generation plants were normal, indicating that the premature aging genes were recessive compared to the normal genes. F 1 Generation gets F through selfing 2 Generation group, count F from heading stage to fruiting stage 2 Population of wild-type and premature aging individuals, premature aging rice plants Ospse1 The separation ratio with wild-type material CW312 is 1:3, indicating that the premature aging phenotype is controlled by a pair of recessive genes.

[0034] Rice plants that will age prematurely Ospse1 Crossed with japonica rice variety Nipponbare...

Example Embodiment

[0038] Example 2 OsPSE1 Genetic complementary vector and overexpression vector to transform rice plants with premature senescence Ospse1

[0039] According to the Nipponbare reference sequence, the specific PCR primers including the coding region of about 1 kb and the upstream and downstream regulatory regions each extend about 2.3 kb and 1.1 kb. OsPSE1 -CT-F and OsPSE1 -CT-R (Table 2), using high fidelity enzyme KOD-Plus combined with long-range PCR (long-range PCR, LR-PCR) technology, using wild-type CW312 gDNA as a template to amplify the target fragment of about 4.3 kb, using Restriction endonuclease Hin d III and Bam After HI cutting, it was cloned into the plant binary vector pCAMBIA1300 (CAMBIA Company, Canberra, Australia) to obtain a genetic complementary vector.

[0040] In addition, the design contains candidate genes OsPSE1 Gene specific primers for the entire ORF OsPSE1 -OE-F and OsPSE1 -OE-R (Table 2), PCR amplification of CW312 genomic DNA to obtain a fragment of ...

Example Embodiment

[0043] Example 3 Rice senescence-related genes OsPSE1 / Ospse1 Nucleotide sequence and protein structure

[0044] Amplification by PCR OsPSE1 with Ospse1 And sequenced the genome sequence and found that rice plants with premature senescence Ospse1 Is wild type OsPSE1 A C base deletion occurred at 947 bp of the coding region ( image 3 a), resulting in the extension of its coding region from 1095 bp to 1191 bp, and the encoded protein has a frameshift mutation from the mutation point, OsPSE1 with Ospse1 The nucleotide sequences of are shown in SEQ ID No:1 and SEQ ID No:3, respectively.

[0045] OsPSE1 And premature aging plants Ospse1 The protein sequences are shown in SEQ ID No: 2 and SEQ ID No: 4, respectively. OsPSE1 It encodes a protein polypeptide composed of 364 amino acid residues, with a molecular weight of about 39.5 kDa and an isoelectric point of 7.432; and Ospse1 It encodes a protein polypeptide composed of 396 amino acid residues, with a molecular weight of about 43....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of gene engineering, and particularly discloses an application of an OsPSE1 gene in plant senescence regulation. According to the application of the OsPSE1 gene in plant senescence regulation, one premature aging rice plant is utilized, the OsPSE1 gene is cloned with a map-based cloning method, functional analysis is performed, and the result shows that the premature aging rice can return to normal through expression of the OsPSE1 gene in premature aging rice, and plant senescence can be further delayed and rice biomass accumulation is increased through overexpression of the OsPSE1 gene in normal rice; and the result proves that the OsPSE1 gene has better application potential in the aspect of increase of crop yield. The gene engineering means is utilized to regulate and control the expression level of the OsPSE1 gene for molecular breeding, and the OsPSE1 gene has potential application value in the aspect of increase of crop yield.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to OsPSE1 The use of genes in the regulation of plant senescence. Background technique [0002] Plant senescence is a process of gradual aging and death from the cell level to the tissue, organ and even the overall level of the plant determined by the growth period of the plant. It is the final stage of plant development. The senescence and death of plants are first manifested on the leaves, because leaves are an important place for plants to use light energy and synthesize organic compounds. The senescence of leaves will directly reduce the photosynthesis and biomass accumulation of crops. Therefore, leaves are an important phenotype for studying plant senescence. [0003] Plant senescence is not only regulated by the expression of internal genes, but also affected by the external environment of plant growth. In recent years, a variety of molecular clonin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/82A01H5/00
Inventor 陈乐天刘耀光吴海斌韩靖鸾谢勇尧王斌陈远玲
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap