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Adsorbent for treating gram bacterial infection and blood perfusion device

A technology of gram bacteria and hemoperfusion device, which is applied in the field of hemoperfusion devices, can solve the problems of limited application, achieve the effects of reducing shedding, good treatment of gram infection, and improvement of immobilization strength

Active Publication Date: 2015-05-20
佛山市博新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Melittin has a strong inhibitory effect on Gram-positive and negative bacteria, but it has a strong hemolytic activity, which limits its clinical application

Method used

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  • Adsorbent for treating gram bacterial infection and blood perfusion device
  • Adsorbent for treating gram bacterial infection and blood perfusion device
  • Adsorbent for treating gram bacterial infection and blood perfusion device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Immobilization of Nisin on Cation Exchange Resin

[0032] Take 10g of cation exchange resin (pore diameter 100nm, specific surface area 60㎡ / g, ion exchange capacity 10mmol / g), add 2.5g Nisin aqueous solution 50mL, shake at room temperature for 8 hours. Then it was washed with water for injection to obtain S1.

Embodiment 2

[0033] Example 2 Immobilization of Nisin on Long Chain Modified Cation Exchange Resin

[0034] 2-1 Long chain modification of cation exchange resin

[0035] Take 10g of cation exchange resin (pore diameter 100nm, specific surface area 60㎡ / g, ion exchange capacity 10mmol / g), add 30mL 10% hexamethylenediamine solution, adjust pH to 4.8, add 0.4g NHS (N-hydroxysuccinyl imine), then slowly add 0.5g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and react with shaking at 25°C for 4 hours. After the reaction is completed, wash with alcohol and purified water.

[0036] Then add 30mL of 10% adipic acid solution, adjust the pH to 4.8, add 0.6g NHS (N-hydroxysuccinimide), and then slowly add 0.5g 1-(3-dimethylaminopropyl)-3 -Ethylcarbodiimide hydrochloride (EDC), shaking reaction at 25°C for 4 hours. After the reaction is completed, wash with alcohol and purified water.

[0037] 2-2 Immobilized Nisin

[0038] Take 10 g of the modified cation exchange res...

Embodiment 3

[0039] Example 3 Immobilization of Polylysine on Long Chain Modified Cation Exchange Resin

[0040] 3-1 Long chain modification of cation exchange resin

[0041] Same as embodiment 2 step 2-1

[0042] 3-2 Immobilized polylysine

[0043] Take 10 g of the modified cation exchange resin, add 50 mL of an aqueous solution of 2.5 g of polylysine, and then add 0.2 g of EDC, and shake at room temperature for 8 hours. Then rinse with water for injection. Gram-adsorbent S3 was obtained.

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Abstract

The invention discloses an adsorbent for treating gram bacterial infection and a blood perfusion device. A carrier of the adsorbent is cation exchange resin or activated polystyrene divinyl phenyl resin, a ligand is Nisin, polylysine or melittin analogue. By selecting a carrier type and immobilizing the Nisin, the polylysine or the melittin analogue with specific effect on the gram bacteria, a purpose of treating the gram bacterial infection is achieved. The blood perfusion device comprises a perfusion machine which is filled with the adsorbent for treating gram bacterial infection, and further comprises a perfusion machine which is filled with an adsorbent for adsorbing inflammatory factors, so that the inflammatory factors in the blood can be effectively removed to achieve effects of relieving and treating. Furthermore, the blood perfusion device comprises a device for dropwise adding sodium lactate injection, so that cooperative effect is generated on inhibition and adsorption of the gram bacteria, and a relatively good effect of treating the gram bacterial infection is achieved.

Description

technical field [0001] The invention relates to the field of blood purification and medical materials, in particular to an adsorbent for treating Gram infection and a blood perfusion device containing the adsorbent. Background technique [0002] Gram bacteria [0003] Gram includes Gram-negative bacteria and Gram-positive bacteria. Most intestinal bacteria belong to Gram-negative bacteria. They produce endotoxin and make people sick by endotoxin; Belonging to Gram-positive bacteria, they can produce exotoxins to make people sick. Common Gram-positive bacteria include: Staphylococcus, Streptococcus, Streptococcus pneumoniae, Bacillus anthracis, Bacillus diphtheria, Tetanus bacilli etc. In the past, many researchers paid more attention to the research of Gram-negative bacterial infection and endotoxemia, but paid little attention to Gram-positive bacterial sepsis. [0004] Large-scale epidemiological data show that sepsis and septic shock caused by Gram-positive bacteria h...

Claims

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Application Information

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IPC IPC(8): B01J20/26A61M1/38
CPCA61M1/3612A61M1/3633A61M1/38B01J20/26B01J2220/44B01J2220/4812
Inventor 李设桥姜建明
Owner 佛山市博新生物科技有限公司
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