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Bacillus cereus J19 as well as induction medium and application thereof

A technology of Bacillus cereus and inducing culture medium, applied in the field of microbial fermentation and application, can solve problems such as poor effect, and achieve the effects of avoiding bacterial drug resistance, reducing the number, and avoiding microecological damage

Inactive Publication Date: 2015-05-20
QINGDAO GENYUAN BIOLOGICAL TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main causes of EMS are highly pathogenic Vibrio parahaemolyticus, algae toxins and adverse environmental factors. Conventional antibiotics, disinfectants and other bactericidal drugs are not effective for this disease

Method used

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  • Bacillus cereus J19 as well as induction medium and application thereof
  • Bacillus cereus J19 as well as induction medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, the acquisition of Bacillus cereus J19 bacterial strain

[0023] In November 2010, the inventor collected the bottom mud of a sea cucumber breeding area in Haiyangsuo Town, Rushan City, Weihai City, and enriched and isolated a strain of Bacillus cereus in the laboratory. The laboratory number is J19, which is described in the present invention. Bacillus cereus J19.

[0024] The Bacillus cereus J19 strain was preserved as a strain. The name of the preservation unit: General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee; Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Chinese Academy of Sciences Institute of Microbiology. Bacillus cereus Bacillus cereus Date of deposit: November 25, 2014; deposit number: CGMCC No. 10052.

[0025] According to the experimental verification of the present invention, it can be known that Bacillus cereus J19 is a beneficial bacterium with the potential ...

Embodiment 2

[0026] Embodiment 2, the selection of different induction medium formulations

[0027] In this embodiment, the medium components glucose 0.1%~1%; soybean meal powder 2.0%~4.0%; yeast powder 0.5%~1%; sodium chloride 0.1%~1%; corn steep liquor 0.1%~1.0% are formulated In the optimization test, the percentages in the medium formula in the present invention are mass ratios. The soybean meal powder needs to be pretreated. The pretreatment method is to take 2g of soybean meal powder with a particle size greater than or equal to 40 mesh, add 3000U of neutral protease, add 100ml of water, stir well, and place it in a water bath shaker at 40°C at 100 rpm for enzymolysis. 2h.

[0028] In the experiment, the conventional dosages of yeast powder, corn steep liquor and sodium chloride were selected, which were 0.5%, 1.0%, and 0.2%, respectively, and further explored whether different glucose dosages and soybean meal needed enzymatic hydrolysis. Antibacterial tests were carried out accord...

Embodiment 3

[0033] Embodiment 3, utilize the bacteriostatic activity of the fermentation product after described substratum fermentation

[0034] In this example, the pathogenic bacteria Vibrio parahaemolyticus 3# was isolated from Penaeus vannamei infected with EMS (Early Mortality Syndrome), and after activation, it was inoculated in 2216E liquid medium at 30°C, 180 rpm Cultivate on a shaking table for 12-24 hours until the cell concentration is 10 8 CFU / ml, take 0.1ml and spread it on the 2216E plate, then place a sterilized Oxford cup on the plate, draw 0.2ml of the sample to be tested into the Oxford cup, place it in a 30°C incubator for 12-24h, and observe the pH Bacterial circle, the inhibition zone of Vibrio parahaemolyticus on the 2216E plate is greater than 15mm.

[0035] Test results such as figure 1 , figure 2 As shown in table 2, from different fermentation time sample bacteriostatic effect tests, it can be seen that the bacteriostatic effect of the fermented liquid begi...

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Abstract

The invention provides bacillus cereus J19 as well as an induction medium and application thereof. The preservation serial number of bacillus cereus J19 is CGMCC No.10052. The induction medium comprises the following components: 0.1-1% of glucose, 2.0-4.0% of bean pulp powder, 0.5-1% of yeast powder, 0.1-1% of sodium chloride and 0.1-1.0% of corn pulp. The induction medium can be used for inducing bacillus cereus J19 to generate activity of inhibiting vibrio parahaemolyticus, the number of pathogenic vibrio parahaemolyticus in an aquaculture environment can be effectively reduced, G+ bacteria are not inhibited, micro-ecological damage and bacterium drug resistance caused in the use process of antibiotics can be avoided, and by adopting an antibiotic liquid produced from bacillus cereus J19, the disease and death risk of aquaculture animals caused by excessive multiplication of vibrios can be reduced by means of ecological methods.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and application, and in particular relates to a strain of Bacillus cereus J19 and its induction medium and application. Background technique [0002] Since 2009, China's shrimp farming industry has been hit hard by Early Mortality Syndrome (EMS). In the first half of 2011, the mortality loss caused by the disease in parts of Hainan, Fujian, Guangdong and Guangxi was as high as 80%. By 2013, South China In many breeding areas, the growth rate of 40 heads / catties of first-made shrimp is only 5-10%, and most shrimp ponds of medium-sized shrimps have drained ponds within 10-20 days of seedlings. The incidence is faster and wider than that of one Make shrimp. The main causes of EMS are highly pathogenic Vibrio parahaemolyticus, algae toxins and adverse environmental factors. Conventional antibiotics, disinfectants and other bactericidal drugs are not effective against the disease. The use of biol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/02A01P1/00C12R1/085
CPCA01N63/10C12P1/04C12N1/205C12R2001/085
Inventor 梁晶晶王娇谢金萍张明俊
Owner QINGDAO GENYUAN BIOLOGICAL TECH GRP
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