DNA sequencing method and system thereof

Abstract

The invention provides a DNA sequencing method and a system thereof. The DNA sequencing method comprises the following steps: obtaining a current change reference value corresponding to each base and mixing a molecular motor with a DNA molecule to be tested to obtain a first mixture; fixing the first mixture on the gate of a field effect transistor; switching on the field effect transistor, measuring the current between the source and the drain of the field effect transistor and setting the measured current as a first initial current; orderly adding different single base solutions to the first mixture to obtain the currents between the source and the drain corresponding to different base solutions, and comparing the obtained currents with the first initial current, respectively, thereby obtaining the current change value corresponding to each base solution; when the current amplitude change value corresponding to one base solution is similar to a current amplitude change reference value corresponding to the base, determining the inclusion of the base; determining the sequence of the base by ranking according to the current time change values taken by the bases to form the current amplitude change values. The sequencing method has the advantages that the production cost is reduced, the signal-to-noise ratio is increased and the DNA sequencing time is shortened.

Description

technical field [0001] The invention relates to the technical field of DNA and semiconductors, in particular to a DNA sequencing method and system thereof. Background technique [0002] DNA sequencing (DNA sequencing, or translated DNA sequencing) refers to the analysis of the base sequence of a specific DNA fragment, that is, the arrangement of adenine (A), thymine (T), cytosine (C) and guanine (G) Way. [0003] At present, different bases are mainly distinguished by fluorescence, ionic current and tunneling current. The fluorescence method is to connect the base to the DNA chain by reading DNA polymerase or DNA ligase with the assistance of fluorescent or chemiluminescent substances. It is determined indirectly by the optical signal released during the above process, which requires an expensive optical monitoring system, and records, stores and analyzes a large number of optical images, which increases the complexity and cost of the instrument. The methods of ionic curre...

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Problems solved by technology

[0004] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a DNA sequencing method and its system, which is used to solve the problem of DNA sequencing in the prior art. Base distinction, resulting in high cost, slow speed, and low integration

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