Application of Anoxetine A in the Preparation of Pesticide Preparations

An insecticidal pesticide preparation, methanol technology, applied in the application, insecticide, acaricide and other directions, can solve the problems of complex method, low yield, no preparation method for annoskotin A, etc., and achieves environmental pollution. Small, good anti-toxic effect

Inactive Publication Date: 2016-08-24
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Annoxactin A is a meta-ditype annonaceous acetogenins compound (as reported by Yong Chen,.Six cytotoxicannonaceous acetogenins from Annona squamosa seeds, Food Chem.2012,135,960-966), the preparation method in the prior art is to adopt Efficient preparation of liquid phase, complex method, low yield, and low efficiency, can not realize industrial production
At present, there is no simple and efficient preparation method of Anoxetin A and the literature reports on its preparation for killing agricultural diseases and insect pests, etc.

Method used

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  • Application of Anoxetine A in the Preparation of Pesticide Preparations
  • Application of Anoxetine A in the Preparation of Pesticide Preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 2kg of custard apple seeds, crush them, and percolate the coarse powder twice with 5 times the amount of ethyl acetate, combine the percolation liquid, recover under reduced pressure below 60°C to obtain the concentrated liquid, let it stand overnight, and centrifuge at 3000r / min to obtain the obtained product The upper layer (123g), mixed with 120g of silica gel, was subjected to 500g silica gel column chromatography, and was eluted with petroleum ether-ethyl acetate gradient, the ratios were 10:1, 5:1, 2:1, 1:1, 0:1 , 10 fractions were collected for each ratio, each fraction was 500 mL, tracked by thin-layer chromatography, 33-40 fractions were combined, concentrated and dried under reduced pressure at less than 60°C. Recrystallize with 5 times the amount of petroleum ether-ethyl acetate (5:1) to obtain 0.2 g of refined anoxactine A. Its molecular weight was determined to be 622.4 by 1H-NMR data and MS, and it was determined to be annulotide anorostecidine A.

Embodiment 2

[0025] Take 1.8kg of custard apple seeds, crush them, and pass the coarse powder through 20 meshes, and perform supercritical carbon dioxide extraction under the conditions of extraction temperature 35°C, extraction pressure 30Mpa, extraction time 3 hours and entrainer ethanol consumption 180mL. The extract was obtained, which was recovered under reduced pressure below 60°C to obtain 98 g of concentrated liquid. After mixing 100g of silica gel, carry out 500g of silica gel column chromatography, and use chloroform-methanol gradient elution, the ratio is 100:1, 50:1, 25:1, 10:1, each ratio collects 10 fractions, each fraction is 500mL , traced by thin layer chromatography, combined 15-21 fractions, concentrated and dried under reduced pressure at less than 60°C. Recrystallize with 5 times the amount of chloroform-methanol (50:1) to obtain 0.23 g of refined anoxetine A. Its molecular weight was determined to be 622.4 by 1H-NMR data and MS, and it was determined to be annulotide...

Embodiment 3

[0026] The biological activity of embodiment 3 Anoxetine A

[0027] 1. Determination of the contact effect on bean aphid:

[0028] Use a micro-dropper to drip 10% distilled acetone aqueous solution containing different concentrations of anoxactin A on the abdomen and back of the bean aphid, and the amount of dripping per insect is 0.05 μL. After the dripping was completed, each was put into a biochemical incubator for cultivation. After 24 hours, the death situation was checked and the death rate was calculated. Immersion with 10% acetone distilled water was used as a control. The mortality rate of the control group was less than 10% for effective determination, and the mortality rate of the treatment group was corrected by Abbott's formula. Measured 3 times in parallel. The test results were analyzed using SPSS13.0 to calculate the lethal concentration LC 50 .

[0029] 2. Determination of contact killing effect on Tetranychus cinnabarinus:

[0030] Paste the double-sid...

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Abstract

The invention discloses an annona squamosa lactone compound annosquatin A prepared from an annona squamosa seed and capable of preventing and controlling agricultural pests. A large number of biological activity assay results show that the annona squamosa lactone compound annosquatin A has poisonous activities in different degrees to the aphis craccivora and cinnabar tetranychid, especially has better poisonous activity to the aphis craccivora than abamectin, and the annosquatin A is extracted from the annona squamosa, the pollution to the environment is small, the annosquatin A is green and environment-friendly, and can be used for developing a new pesticide preparation for preventing and controlling agricultural pests.

Description

technical field [0001] The invention relates to the preparation of m-type annonaceous lactone compounds from the seeds of the traditional Chinese medicine custard apple, and the application of anoxactine A in the preparation of pesticide preparations. Background technique [0002] At present, there are about 500 kinds of artificially synthesized chemical pesticides. The widespread use of these pesticides not only causes serious environmental pollution, but also causes harm to human health. Botanical pesticides are an important part of biological pesticides. Botanical pesticides have the advantages of fast biodegradation in the environment, low biological toxicity to humans and animals, and less resistance to pests and diseases. Searching for compounds with pesticide activity, especially novel molecular structure and special mechanism of action, from traditional Chinese medicines has become an important approach for the research of new pesticides. With the advancement of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/08A01P7/02A01P7/04
Inventor 陈勇李祥陈建伟邱燕
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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