Method for testing content of galactooligosaccharide in formula milk powder
A technology of galacto-oligosaccharide and formula milk powder, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple operation and good extraction effect.
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Embodiment 1
[0065] Embodiment 1 Gas chromatography-mass spectrometry determination of galacto-oligosaccharide content in infant formula milk powder
[0066] (1) Extraction: Weigh 1.0g of the sample (accurate to 0.0001g) into a 50mL graduated plastic centrifuge tube, add 40mL of phosphate buffer solution (0.2M phosphate buffer: 22.0g of potassium dihydrogen phosphate + 6.0g of dihydrogen phosphate Potassium + 1.0L secondary water) dissolved, vortexed to mix. Shake continuously in a water bath at 80°C±2°C for 30min, and after cooling to room temperature, dilute to 100mL with phosphate buffer solution. Pipette 5.0mL of sample extraction solution into a 50mL volumetric flask, dilute to 50mL with phosphate buffer solution, and set aside.
[0067] (2) Enzymolysis: Pipette 1.0mL of the extract into a 10mL volumetric flask at the same time, add 0.5mL of β-galactosidase solution (50U / mL), mix well, keep shaking in a water bath at 50°C±2 for 60min, and cool to After room temperature, dilute to 10...
Embodiment 2
[0084] Example 2 Gas chromatography-mass spectrometry determination of galactooligosaccharides in the second stage of infant formula goat milk powder
[0085] (1) Sample extraction: Weigh 1.0003g and 1.0010g of the second stage of infant formula goat milk powder respectively, add 40mL phosphate buffer solution to a 100mL volumetric flask to dissolve, and vortex to mix. Shake continuously in a water bath at 80°C±2°C for 30min, and after cooling to room temperature, dilute to 100mL with phosphate buffer solution. Pipette 5.0mL of sample extraction solution into a 50mL volumetric flask, dilute to 50mL with phosphate buffer solution, and set aside.
[0086] (3) Enzymolysis: Pipette 1.0mL of the extract into a 10mL volumetric flask, add 0.5mL of β-galactosidase solution (50U / mL), mix well, keep shaking in a water bath at 50°C for 60min, cool to room temperature and use Dilute to 10 mL with 20% acetonitrile, shake well, centrifuge at 10,000 r / min for 10 min, filter the upper aqueou...
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