Method for testing content of galactooligosaccharide in formula milk powder

A technology of galacto-oligosaccharide and formula milk powder, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple operation and good extraction effect.

Active Publication Date: 2015-05-27
谱尼测试集团股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no method for the determination of galactooligosaccharid...

Method used

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  • Method for testing content of galactooligosaccharide in formula milk powder
  • Method for testing content of galactooligosaccharide in formula milk powder
  • Method for testing content of galactooligosaccharide in formula milk powder

Examples

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Embodiment 1

[0065] Embodiment 1 Gas chromatography-mass spectrometry determination of galacto-oligosaccharide content in infant formula milk powder

[0066] (1) Extraction: Weigh 1.0g of the sample (accurate to 0.0001g) into a 50mL graduated plastic centrifuge tube, add 40mL of phosphate buffer solution (0.2M phosphate buffer: 22.0g of potassium dihydrogen phosphate + 6.0g of dihydrogen phosphate Potassium + 1.0L secondary water) dissolved, vortexed to mix. Shake continuously in a water bath at 80°C±2°C for 30min, and after cooling to room temperature, dilute to 100mL with phosphate buffer solution. Pipette 5.0mL of sample extraction solution into a 50mL volumetric flask, dilute to 50mL with phosphate buffer solution, and set aside.

[0067] (2) Enzymolysis: Pipette 1.0mL of the extract into a 10mL volumetric flask at the same time, add 0.5mL of β-galactosidase solution (50U / mL), mix well, keep shaking in a water bath at 50°C±2 for 60min, and cool to After room temperature, dilute to 10...

Embodiment 2

[0084] Example 2 Gas chromatography-mass spectrometry determination of galactooligosaccharides in the second stage of infant formula goat milk powder

[0085] (1) Sample extraction: Weigh 1.0003g and 1.0010g of the second stage of infant formula goat milk powder respectively, add 40mL phosphate buffer solution to a 100mL volumetric flask to dissolve, and vortex to mix. Shake continuously in a water bath at 80°C±2°C for 30min, and after cooling to room temperature, dilute to 100mL with phosphate buffer solution. Pipette 5.0mL of sample extraction solution into a 50mL volumetric flask, dilute to 50mL with phosphate buffer solution, and set aside.

[0086] (3) Enzymolysis: Pipette 1.0mL of the extract into a 10mL volumetric flask, add 0.5mL of β-galactosidase solution (50U / mL), mix well, keep shaking in a water bath at 50°C for 60min, cool to room temperature and use Dilute to 10 mL with 20% acetonitrile, shake well, centrifuge at 10,000 r / min for 10 min, filter the upper aqueou...

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Abstract

The invention discloses a method for testing content of galactooligosaccharide, and in particular relates to a method for testing content of galactooligosaccharide in formula milk powder by means of gas chromatography-mass spectrum. The method comprises the following steps: extracting galactooligosaccharide and lactose from samples to be detected by using hot phosphate buffer liquid; hydrolyzing and centrifuging galactooligosaccharide in extracting liquid by using beta-galactosidase to obtain liquid supernatant; enabling the liquid supernatant to pass through a microfiltration membrane; carrying out nitrogen blowing or performing low-temperature vacuum drying; deriving by using BSTFA and making up to the volume by using acetone. The content of dissociative galactose and lactose in extracting raw liquid and the total quantity of galactooligosaccharide obtained by enzymolysis on decomposition liquid and galactose released by lactose are tested by utilizing a gas phase-mass spectrum combined method, and the content of the galactooligosaccharide in samples is calculated by using the content of galactose content obtained by performing enzymolysis on decomposition liquid to substract the content of galactose obtained by extracting the raw liquid and the content of galactose produced by lactase enzymolysis. The content of galactooligosaccharide in the milk powder is detected by using the method, and the method has the advantages of extremely-low detection and high sensitivity.

Description

[0001] The method relates to a detection method for determining the content of galacto-oligosaccharides in formula milk powder by gas chromatography-mass spectrometry, and belongs to the field of dairy product detection. Background technique [0002] Galactooligosaccharide, English name Galactooligosaccharide, referred to as GOS, is a functional oligosaccharide with natural properties. Its molecular structure is generally 1-7 galactose connected to galactose (Gal) or glucose (Glc) molecules The base, namely Gal-(Gal)n-Glc / Gal (n is 0-6), the molecular weight is about 300-2000. The chemical structural formula of galactooligosaccharides is as follows: [0003] [0004] The chemical structure of galactooligosaccharides [0005] Galacto-oligosaccharides are abundant in human breast milk, and the establishment of bifidobacteria in infants largely depends on the GOS components in breast milk. GOS cannot be digested and absorbed by the human digestive tract, but can directly ent...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 宋薇
Owner 谱尼测试集团股份有限公司
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