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In-situ solid fermentation method for agricultural bacillus microbial inoculum

A technology of bacillus and solid fermentation, which is applied in the field of in-situ solid fermentation of agricultural bacillus inoculants, can solve the problems of immature solutions of solid fermentation methods and product marketization, etc., and achieves high-value utilization and equipment requirements. Low, high choice effect

Active Publication Date: 2015-06-03
JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although various manufacturers are actively exploring the solid fermentation technology of Bacillus in recent years, so far, the traditional solid fermentation method has not There are mature solutions and products on the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for in-situ solid fermentation of an agricultural bacillus bacterial agent, comprising the steps of:

[0036] (1) Sterilize the fermented material in a rotary sterilizer: the method is damp heat sterilization at a temperature of 121°C for 4 hours;

[0037] The composition of above-mentioned fermented material comprises:

[0038] 4.5 parts of quick-acting carbon source: specifically, 2 parts of bean flour, 1 part of corn meal, 1 part of peanut meal, and 0.5 parts of glucose;

[0039] 8 parts of long-term carbon sources: specifically mushroom residue 3, furfural residue 2, chicken manure 2, rice husk 1;

[0040] 4 parts of nitrogen source: specifically corn steep liquor 1, yeast powder 1, distiller's grains 2;

[0041] Dipotassium hydrogen phosphate 0.5%

[0042] Potassium dihydrogen phosphate 0.3% Magnesium sulfate 0.1%

[0043] Manganese sulfate 0.008% water 50%

[0044] Adjust the pH of the material to 7.0;

[0045] After the sterilization is completed, ...

Embodiment 2

[0051] A method for in-situ solid fermentation of an agricultural bacillus bacterial agent, comprising the steps of:

[0052] (1) Sterilize the fermented material in a rotary sterilizer: the method is moist heat sterilization, the temperature is 121°C, and the sterilization is 3.5h;

[0053] The composition of above-mentioned fermented material comprises:

[0054] The available carbon source is 2.5 parts glucose

[0055] The long-term carbon source is 8 parts, specifically furfural slag and cotton firewood with a weight ratio of 2:1;

[0056] 3 parts of nitrogen source, specifically corn steep liquor:ammonium sulfate with a weight ratio of 1:1;

[0057] Dipotassium hydrogen phosphate 0.6%

[0058] Potassium dihydrogen phosphate 0.4% Magnesium sulfate 0.08%

[0059] Manganese sulfate 0.009% water 55%

[0060] Adjust the pH of the material to 6.8-7.2;

[0061] After the sterilization is completed, the material is placed in the sterilizer without moving, and the temperature i...

Embodiment 3

[0067] (1) Sterilize the fermented material in a rotary sterilizer: the method is damp heat sterilization at a temperature of 121°C for 2 hours;

[0068] The composition of above-mentioned fermented material comprises:

[0069] 1 part of quick-acting carbon source, specifically 0.5 part of bean flour and 0.5 part of glucose;

[0070] 1 part of long-term carbon source, specifically 0.5 part of mushroom dregs, 0.5 part of rice husk;

[0071] 1 part of nitrogen source, specifically 0.5 part of peptone, 0.5 part of peanut cake powder;

[0072] Dipotassium hydrogen phosphate 0.2%

[0073] Potassium dihydrogen phosphate 0.1% Magnesium sulfate 0.05%

[0074] Manganese sulfate 0.006% water 40%

[0075] Adjust the pH value of the material to 6.8;

[0076] After the sterilization is completed, the material is placed in the sterilizer without moving, and the temperature is lowered to 30°C.

[0077] (2) Inoculate the Bacillus strain into the sterilizer equipped with sterilized mater...

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Abstract

The invention belongs to the technical field of microbial inoculum fermentation and particularly discloses an in-situ solid fermentation method for agricultural bacillus microbial inoculum. The fermenting method provided by the invention comprises the following steps: sterilizing a fermentation material in a rotary sterilizer, inoculating the bacillus strain to a sterilizer loaded with a sterilized material, and performing in-situ solid fermentation; and baking the fermentation material at 40-60 DEG C till the water content is less than 35%. A rotary sterilizer provided by the invention is used for in-situ fermentation, compared with the traditional fermentation mode, the cost of the fermentation material is lower, the fermentation process is simpler and easier to control and the labor cost is saved; the floor area of the whole production process is smaller, the yield in unit volume is higher than that of the traditional fermentation mode and the energy consumption is lower; and the whole matrix can be used as a compound fertilizer raw material after fermenting is finished, the further recovery and the purification are unnecessary and no waste is generated. The cost of the microbial inoculum prepared by the invention is 40-70% of the liquid fermentation microbial inoculum.

Description

technical field [0001] The invention belongs to the technical field of fermentation of microbial inoculum, and in particular relates to an in-situ solid fermentation method of agricultural bacillus inoculum. Background technique [0002] Bacillus are a class of aerobic and facultatively anaerobic, Gram-positive rod-shaped bacteria that produce stress-resistant endospores. The fungus is widely distributed in nature, easy to isolate and cultivate, harmless to humans and animals, and friendly to the environment. Bacillus grows fast, has simple nutrition, and can produce heat-resistant and stress-resistant spores. The spores have strong stress resistance and are conducive to preservation. The damage caused by , ultraviolet rays, etc., this biological feature makes it an ideal agricultural microorganism. After the bacillus bacteria agent is applied to the farmland, it can fully decompose the insoluble macromolecular com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125C12R1/11C12R1/12C12R1/07
CPCC12N1/20
Inventor 李昆仑赵林孙欣马培铭高海燕
Owner JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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