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Preparation method of whey protein peptide with antibacterial activity

A technology of whey protein peptide and antibacterial activity, applied in the biological field, can solve the problems of uncertain genetic engineering expression system, small-scale separation and extraction method, and high chemical synthesis, achieve significant antibacterial effect, increase weight and safety, and automate high degree of effect

Inactive Publication Date: 2015-06-03
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The development and application of antimicrobial peptides has attracted widespread attention from scholars, and the most common method is to use chemical synthesis and genetic engineering to prepare antimicrobial peptides. However, the separation and extraction of antimicrobial peptides from food protein sources is small in scale, complex in operation, and high in cost for chemical synthesis. The existence of problems such as low efficiency and uncertain genetic engineering expression system limit the application of food additive antimicrobial peptides in food processing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of the whey protein peptide with antibacterial activity, its operation steps are as follows:

[0023] a. Pretreatment: Mix 1 part of food-grade whey protein powder with 3 parts of water, keep the temperature at 80°C for 5 minutes, then cool down to 45°C, adjust the pH of the solution to 7.0 with NaOH solution, and make a whey protein solution;

[0024] b. Enzymolysis: Add 0.001 part of trypsin to the whey protein solution prepared in step a, and maintain the pH of the system at 7.0 with NaOH solution, stir at 45°C for 2.0h, then heat up to 85°C , keep the temperature for 10min to prepare whey protein enzymatic hydrolyzate;

[0025] c. Separation: remove the solid matter in the whey protein hydrolyzate prepared in step b, collect the filtrate in the enzymolyzate with a molecular weight between 3000Da and 5000Da, and obtain crude whey protein peptide;

[0026] d. Purification: The crude whey protein peptide is prepared into a mixed liquid with a ma...

Embodiment 2

[0029] The preparation method of the whey protein peptide with antibacterial activity, its operation steps are as follows:

[0030] a. Pretreatment: Mix 1 part of food-grade whey protein powder with 20 parts of water, keep the temperature at 90°C for 5 minutes, then cool down to 60°C, adjust the pH of the solution to 8.5 with NaOH solution, and make a whey protein solution;

[0031] b. Enzymolysis: add 0.005 parts of trypsin to the whey protein solution prepared in step a, and maintain the pH of the system at 8.5 with NaOH solution, stir at 60°C for 8.0 hours, and then heat up to 100°C , keep the temperature for 15min to prepare whey protein enzymatic hydrolyzate;

[0032] c. Separation: remove the solid matter in the whey protein hydrolyzate prepared in step b, collect the filtrate in the enzymolyzate with a molecular weight between 3000Da and 5000Da, and obtain crude whey protein peptide;

[0033] d. Purification: The crude whey protein peptide is prepared into a mixed liqu...

Embodiment 3

[0036] The preparation method of the whey protein peptide with antibacterial activity, its operation steps are as follows:

[0037] a. Pretreatment: Mix 1 part of food-grade whey protein powder with 3 parts of water, keep the temperature at 80°C for 5 minutes, then cool down to 45°C, adjust the pH of the solution to 7.0 with NaOH solution, and make a whey protein solution;

[0038] b. Enzymolysis: add 0.002 parts of trypsin to the whey protein solution prepared in step a, and maintain the pH of the system at 7.0 with NaOH solution, stir at 45°C for 4.0h, and then heat up to 85°C , keep the temperature for 10min to prepare whey protein enzymatic hydrolyzate;

[0039] c. Separation: remove the solid matter in the whey protein hydrolyzate prepared in step b, collect the filtrate in the enzymolyzate with a molecular weight between 3000Da and 5000Da, and obtain crude whey protein peptide;

[0040]d. Purification: The crude whey protein peptide is prepared into a mixed liquid with ...

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Abstract

The invention discloses a preparation method of a whey protein peptide with antibacterial activity, and relates to the technical field of biology. The preparation method comprises steps of preprocessing, enzymolysis, separation and purification, wherein the purification step adopts a simulated mobile chromatography separation method for industrial production of the coarse whey protein peptide after separation, and the whey protein peptide with antibacterial activity is obtained. According to the preparation method, trypsin is adopted to hydrolyze the whey protein to prepare the peptide with antibacterial activity, so that deep processing of the whey protein is realized; the process of the preparation method is simple, the antibacterial activity of the hydrolysis product is high, and the preparation method is suitable for industrial popularization and production; the method is high in automation degree, low in production cost and high in extraction efficiency, and the antibacterial peptide prepared with the method not only has more remarkable bacteriostatic effect, but also is safe and nutritional, provides a reliable base material or ingredient for development of a natural preservative agent, can increase the weight of preservative products of China and improve the safety of the preservative products of China, and meets the requirement of market development of natural food additives.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of whey protein peptide with antibacterial activity. Background technique [0002] Whey is a by-product of industrial cheese production and casein, and is a translucent liquid that is expelled during curdling. Whey protein contains a variety of peptide sequences with different biological activities. Under the action of exogenous proteases, the precursor substances without biological activity are released. These small peptide molecules can not only provide the nutrients and nutrients needed for human growth and development. Energy, and more importantly, it also has potential antibacterial, cholesterol-lowering, blood-pressure-lowering, anti-oxidation, growth-promoting, immune-enhancing, sedative, and substance-improving functions. The development of protein peptides with antibacterial activity using whey protein as a raw material can provide a basis for the compr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/16
Inventor 刘妍妍王宪青张丽萍刁静静
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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