Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nerve conduit and preparation method thereof

A technology of nerve conduit and electrospinning method, which is applied in the direction of human tubular structure devices, medical science, prosthesis, etc., can solve the problems of reduced activity and loss of biologically active substances, and achieve the avoidance of fibrosis, low cost, and process steps simplified effect

Inactive Publication Date: 2015-06-10
邹蓉
View PDF5 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the methods used in these patents all require direct mixing of various biologically active substances with organic solvents, resulting in reduced or lost activity of the biologically active substances

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nerve conduit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] A kind of preparation method of nerve guide provided by the present invention comprises the following steps:

[0042] Step (1) prepares a hydrophilic polymer solution and a hydrophobic polymer solution;

[0043] Step (2) adding the hydrophilic polymer solution into the syringe of the electrospinning device, and spinning to obtain the inner layer 1 of the nerve guide;

[0044] Step (3) Place and fix two steel needles on the inner layer, then add the hydrophobic polymer solution into the syringe of the electrospinning device, and perform spinning to obtain the outer layer 2 of the nerve guide;

[0045] Step (4) cleaning the inner and outer layers of the prepared nerve guide, immersing in alcohol, removing the steel needle, and then obtaining the nerve guide, sterilizing and packaging.

Embodiment 1

[0047] A kind of preparation method of nerve guide provided by the present invention comprises the following steps:

[0048] (1) Select hydrophilic gelatin and silk fibroin, the mass ratio of the two is 70:30, the solvent is formic acid, the mass fraction of the spinning solution is 15%, the spinning solution is added to the syringe of the electrospinning device, and the micro amount is adjusted. The speed of the syringe pump is 8ml / h, the receiving distance is 10cm, and the voltage is 20kV. Turn on the electrospinning machine and perform spinning to obtain the inner layer of the cell scaffold with an average fiber diameter of 800-1000nm;

[0049] (2) Select the polymer material of hydrophobic L-polylactic acid and ε-caprolactone copolymerization, the mass ratio of the two is 50:50, the number average molecular weight is 310000, and it is dissolved in hexafluoroisopropanol to obtain a mass fraction of 10% spinning solution, place two steel needles with a diameter of 0.05mm on ...

Embodiment 2

[0052] A kind of preparation method of nerve guide provided by the present invention comprises the following steps:

[0053] (1) Select the polymer material of hydrophilic chondroitin sulfate and hyaluronic acid, the mass ratio of the two is 70:30, with trifluoroethanol as solvent, the mass fraction of spinning solution is 20%, the spinning solution is added In the syringe of the electrospinning device, adjust the speed of the micro-injection pump to 8ml / h, the receiving distance to 10cm, and the voltage to 15kV, turn on the electrospinning machine, and perform spinning to obtain the inner layer of the cell scaffold with an average fiber diameter of 1000-1500nm ;

[0054] (2) The ratio of hydrophobic lactic acid and glycolic acid is selected to be 10:90, which is a copolymerized polymer material with a number average molecular weight of 350,000, which is dissolved in trifluoroethanol to obtain a spinning solution with a mass fraction of 14%, and then the above-mentioned Add t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Average pore sizeaaaaaaaaaa
Average pore sizeaaaaaaaaaa
Tube wall thicknessaaaaaaaaaa
Login to View More

Abstract

The invention provides a nerve conduit. The nerve conduit is characterized by comprising an inner layer, an outer layer and a cavity; the outer surface of the inner layer is wrapped with the outer layer; the cavity is formed between the inner layer and the outer layer; the inner layer is a hydrophilic cytoskeleton layer manufactured by the electrostatic spinning method; the outer layer is a hydrophobic nerve conduit skeleton layer by the electrostatic spinning method; the cavity is used for storing a bioactive factor solution. The nerve conduit has the characteristics of being proper in strength and hardness, degradable, capable of providing proper neurotrophic active substances, ideal in double-layer or multi-layer structure, semi-permeable, low in cost, short in production cycle, easy to be stored and transported, free of viruses, free of immunological rejection or extremely small in immunological rejection, and wide in applicable scope; the requirement on nerve regeneration process can be met well.

Description

technical field [0001] The invention belongs to the field of biomedical materials and implantable medical devices, and in particular relates to a nerve guide and a preparation method thereof. Background technique [0002] The nervous system is one of the most important organs in the human body, which controls the sensory and motor functions of the human body. Various traumas such as compression, stretching, tearing, cutting, and other factors such as ischemia, tumors, etc. will cause partial or complete damage to the nervous system, resulting in loss of function and other neurological diseases. Autologous nerve transplantation is considered to be the gold standard for the treatment of nerve defects. However, autologous nerve transplantation has limited sources of donor nerves, requires two surgeries, remains of varying degrees of dysfunction at the donor site, and incompatibility between the donor nerve and the recipient nerve. Shortcomings such as matching limit its clinic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/54A61L27/40A61F2/04
Inventor 邹蓉曲晓宁
Owner 邹蓉
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com