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A nano-artificial dura mater that can simultaneously serve as a drug sustained-release system and its preparation method

An artificial dura mater and nano-technology, applied in the field of biomedicine, can solve the problem of no artificial meninges

Active Publication Date: 2015-07-29
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Second, limited by the method of drug addition
[0014] In short, there is currently no artificial meninges that can meet the comprehensive quality requirements of general artificial meninges while effectively mixing drugs

Method used

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  • A nano-artificial dura mater that can simultaneously serve as a drug sustained-release system and its preparation method
  • A nano-artificial dura mater that can simultaneously serve as a drug sustained-release system and its preparation method
  • A nano-artificial dura mater that can simultaneously serve as a drug sustained-release system and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Preparation of anti-adhesion layer: choose hydrophobic polycaprolactone, chloroform / methanol mixed solvent ratio of 1:1, mix ceftriaxone sodium at a concentration of 1%. Mix the hemostatic drug promethazine to a final concentration of 10 mg / ml. A homogeneous solution was obtained.

[0058]Add the above solution into the syringe of the electrospinning device, adjust the rate of the micro-injection pump to 0.8 ml / hour, adjust the voltage of the high-voltage generator to 12KV, adjust the receiving distance of the receiving device to 15 cm, and receive the fiber into a film-like structure . A fiber diameter of 600 nm was obtained.

[0059] Turn off the electrostatic device.

[0060] (2) Preparation of cell scaffold layer: use hydrophilic silk fibroin and natural gelatin in a ratio of 20-80:80-20, and the mass fraction of spinning solution is 9%.

[0061] Prepare basic fibroblast cytokine solution, mix it with the above electrospinning solution evenly, so that the fi...

Embodiment 2

[0064] (1) Preparation of anti-adhesion layer: choose hydrophobic polycaprolactone, chloroform / methanol mixed solvent ratio of 1:1, mix ceftriaxone sodium at a concentration of 1%. Mix the hemostatic drug promethazine to a final concentration of 10 mg / ml.

[0065] Add the above solution into the syringe of the electrospinning device, adjust the rate of the micro-injection pump to 0.8 ml / hour, adjust the voltage of the high-voltage generator to 12KV, adjust the receiving distance of the receiving device to 15 cm, and receive the fiber into a film-like structure . A fiber diameter of 600 nm was obtained.

[0066] Turn off the electrostatic device.

[0067] (2) Preparation of the transition layer: the following scheme is selected in this embodiment: the mass ratio of polyurethane to hyaluronic acid is 70:30, and the mass fraction of spinning solution is 10%. Mix ampicillin at a concentration of 3%. A homogeneous solution was obtained.

[0068] Turn on the electrospinning, an...

Embodiment 3

[0075] (1) Preparation of anti-adhesion layer: choose hydrophobic polycaprolactone, chloroform / methanol mixed solvent ratio of 1:1, mix ceftriaxone sodium and 1% concentration. Mix the hemostatic drug promethazine to a final concentration of 10 mg / ml. A homogeneous solution was obtained.

[0076] Add the above solution into the syringe of the electrospinning device, adjust the rate of the micro-injection pump to 0.8 ml / hour, adjust the voltage of the high-voltage generator to 12KV, adjust the receiving distance of the receiving device to 15 cm, and receive the fiber into a film-like structure . A fiber diameter of 600 nm was obtained.

[0077] Turn off the electrostatic device.

[0078] (2) Preparation of cell scaffold layer: use hydrophilic silk fibroin and natural gelatin in a ratio of 20-80:80-20, and the mass fraction of spinning solution is 9%.

[0079] Put the above solution into the syringe of the electrospinning device, the receiving distance is 10cm, the voltage i...

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Abstract

The invention provides a nano artificial dura mater capable of being used as a medicine sustained-release system, having the structure which comprises at least two layers, i.e. a hydrophobic anti-blocking electro-spun layer which faces the cerebrum, and a hydrophilic nano cytoskeleton layer which backs on to the cerebrum; and cell factors and / or medicines are arranged in any layer of the artificial dura mater by way of blended spinning. The invention also provides a method for preparing the nano biomimic artificial dura mater. Compared with an artificial mater prepared by the single utilization of the electro-spinning technology, the artificial mater to which the medicines and the cell factors are added by blending technology can effectively prevent infection and faster promote the regeneration process of the artificial mater. The invention also provides a novel medicine loading and releasing mode for treating cerebral diseases, the loaded medicines can be directly and efficiently transferred into the cranial cavity along with the implantation of the dura mate and can be released according to requirements, and therefore the invention realizes favorable treatment effect and has broad application prospect.

Description

technical field [0001] The invention relates to a nano-artificial dura mater which can be used as a drug slow-release system and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Dural defects are common in neurosurgery clinical work. Open craniocerebral injuries (industry, traffic, war, etc.), tumor erosion, congenital meningeal defects and other craniocerebral diseases can cause dural defects. The dural defect needs to be repaired in time to prevent cerebrospinal fluid from overflowing, to prevent the swelling of the brain and the compression of atmospheric pressure, otherwise it will endanger human life. Dural defects can also cause complications such as intracranial infection, brain adhesions, and subcutaneous fluid, often causing diseases such as epilepsy, headache, and brain dysfunction. [0003] Although there are many substitutes for dura mater, the materials for repairing dura mater can be divided into four...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/44A61L27/54A61L27/56
Inventor 徐弢袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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