A carbon-base fluorescence probe for a targeting living cell mitochondrion and preparation thereof

A fluorescent probe and mitochondrial technology, applied in the direction of fluorescence/phosphorescence, luminescent materials, chemical instruments and methods, etc., can solve the problems of poor water solubility, easy fluorescence quenching, phototoxicity, etc., and achieve small particle size and good photostability , good biocompatibility

Inactive Publication Date: 2015-06-10
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although they have been widely used at present, they still have disadvantages such as poor water solubility, easy quenching of fluorescence, and phototoxicity (Yang, Y., et al., Chem Commun, 2012.48(3):380-2).

Method used

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  • A carbon-base fluorescence probe for a targeting living cell mitochondrion and preparation thereof
  • A carbon-base fluorescence probe for a targeting living cell mitochondrion and preparation thereof
  • A carbon-base fluorescence probe for a targeting living cell mitochondrion and preparation thereof

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Embodiment

[0028] (1) Preparation and separation and purification of CDs:

[0029] Dissolve 0.3 mol of citric acid and 1.8 mol of urea in 20ml of deionized water, stir evenly, transfer the solution to a hydrothermal reaction kettle, heat in a muffle furnace, and program the temperature to 200°C, the reaction time is 10 hours, and wait to cool down to room temperature Afterwards, the obtained golden yellow solution was concentrated by freeze-drying method, separated and purified with Sephadex G-50 gel column to remove unreacted substances, and then the product was freeze-dried for future use.

[0030] (2) Synthesis and separation and purification of TPP-CDs:

[0031] Weigh 65mg CDs and 41mg TPP respectively, dissolve them in 5ml deionized water and ethanol respectively, add EDC·HCl and NHS to the ethanol solution of TPP under stirring state (molar ratio TPP:EDC:NHS=1:1.1:1.2 ), after activating the carboxyl group for 20 min, the CDs solution was slowly added dropwise to the TPP solution,...

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Abstract

Preparation of a novel living cell mitochondrion probe based on carbon dots (CDs) is disclosed. A product TPP-CDs is prepared by synthesizing the CDs surfaces of which are provided with amino by adopting citric acid and urea as raw materials through a hydrothermal method, grafting with a triphenylphosphine (TPP) small molecule the tail end of which is provided with carboxyl through an amidation reaction, and separating and purifying by utilization of an aqueous membrane and a glucan G-25 gel column. The mitochondrion probe is simple in preparation method and capable of large-scale production, has good water solubility, good light stability and wide excitation and emission spectrum ranges, and breaks the current situation that mitochondrion probes at present generally are poor in water solubility and liable to fluorescence quenching. In addition, the mitochondrion probe is low in cell toxicity and biological-friendly, and can be successfully used for mitochondrion imaging of living cells.

Description

technical field [0001] The invention relates to fluorescent nanomaterials, in particular to the preparation and application of a mitochondrial targeting probe based on carbon dots (CDs). Background technique [0002] As a new fluorescent probe, CDs not only have the excellent properties of traditional quantum dots, such as wide excitation spectrum, small particle size, good photostability, large Stokes shift, good biocompatibility, and long fluorescence lifetime, but also have low Toxicity characteristics, so it is regarded as a powerful substitute for quantum dots in biomarkers (Ruedas-Rama, M.J., et al., Anal Chim Acta, 2012, 751: 1-23.). [0003] As the energy factory of cells, mitochondria are involved in important processes such as oxidative phosphorylation and lipid oxidation in cells. If it is dysfunctional, it can seriously affect the state of cells, and living organisms show symptoms such as Friedrich's ataxia, Parkinson's disease, diabetes and Huntington's disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64
Inventor 谭明乾王贝贝马小军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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