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Method for orderly assembling DNA and graphene on gold electrode surface

An electrode surface, gold electrode technology, applied in the field of biosensors and electrochemical biosensors, can solve problems such as unreported, and achieve the effects of simple production process, low production cost and good electrochemical performance

Inactive Publication Date: 2015-06-10
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the development of electrochemical detection technology, many methods of graphene and its derivatives modified electrodes have emerged, such as electrochemical reduction of graphene oxide modified glassy carbon electrodes, carboxylated graphene modified glassy carbon electrodes, carbon Nanotube-graphene nanosheet composite films modify gold electrodes, etc., but the graphene derivatives used are still mainly graphene oxide, and the method of using graphene quantum dots (GQDs) and DNA layer-by-layer assembly to modify electrodes has not been seen. to report

Method used

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  • Method for orderly assembling DNA and graphene on gold electrode surface
  • Method for orderly assembling DNA and graphene on gold electrode surface
  • Method for orderly assembling DNA and graphene on gold electrode surface

Examples

Experimental program
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Embodiment 1

[0029] 1) Preparation of ethyl-(3-dimethylpropyl)carbodiimide hydrochloride (EDC) solution: dissolve EDC powder in secondary water and dilute with secondary water to obtain 0.1mol / L EDC solution .

[0030] 2), preparation of N-hydroxysuccinimide (NHS) solution: dissolve NHS powder in secondary water, dilute with secondary water to obtain 0.04mol / L NHS solution;

[0031] 3), the preparation of phosphate buffered saline solution (PBS): get 0.2328g disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 12H 2 O), 0.0897g sodium dihydrogen phosphate dihydrate (NaH 2 PO 4 2H 2 (0), 0.58g sodium chloride (NaCl) in 250ml volumetric flask, gets 5mM PBS buffer solution with secondary water dilution;

[0032] 4), configuration of double-stranded DNA (dsDNA) solution: 4 single-stranded DNAs of different sequences (ssDNA-1 are modified by thiol at the 5' end; ssDNA-2, ssDNA-3, ssDNA-4 are modified by Amino modification) powder was centrifuged in a centrifuge at 6000rpm for 5min, and ...

Embodiment 2

[0040] Steps 1) to 6) are the same as in Example 1.

[0041]7) Immerse the treated gold electrode in the dsDNA-1 solution for 14 hours, then rinse the surface of the modified gold electrode with secondary water to remove free dsDNA-1, and dry it with nitrogen gas for later use. When the electrode surface state is DNA / Au;

[0042] 8) Add 10 μl EDC and 5 μl NHS to 100 μl 1 mg / ml graphene quantum dot solution, then immerse the gold electrode modified with dsDNA-1 for 6 hours, and then wash the surface of the modified gold electrode twice Rinse with water to remove free GQDs, and blow dry with nitrogen for later use. At this time, the surface state of the electrode is GQDs / DNA / Au;

[0043] 9) Add 10 μl EDC and 5 μl NHS to 100 μl dsDNA-2 solution, then immerse the gold electrode modified with dsDNA-1 and graphene quantum dots for 6 hours, and then wash the surface of the modified gold electrode with Rinse with water twice to remove free dsDNA-2, and dry it with nitrogen gas for l...

Embodiment 3

[0046] Step 1) to 6) are the same as embodiment 1.

[0047] 7) Immerse the treated gold electrode in the dsDNA-1 solution for 18 hours, then rinse the surface of the modified gold electrode with secondary water to remove free dsDNA-1, and dry it with nitrogen gas for later use. When the electrode surface state is DNA / Au;

[0048] 8) Add 10 μl EDC and 5 μl NHS to 100 μl 1 mg / ml graphene quantum dot solution, then immerse the gold electrode modified with dsDNA-1 for 6 hours, and then wash the surface of the modified gold electrode twice Rinse with water to remove free GQDs, and blow dry with nitrogen for later use. At this time, the surface state of the electrode is GQDs / DNA / Au;

[0049] 9) Add 10 μl EDC and 5 μl NHS to 100 μl dsDNA-2 solution, then immerse the gold electrode modified with dsDNA-1 and graphene quantum dots for 6 hours, and then wash the surface of the modified gold electrode with Rinse with water twice to remove free dsDNA-2, and dry it with nitrogen gas for l...

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Abstract

The invention relates to a new biosensing method for orderly assembling DNA and graphene quantum dots on a gold electrode surface through chemical bond combination, belonging to the field of a chemicobiological sensor. The method is characterized in that DNA-terminal modified amino groups and GQDs edge carboxy groups are subjected to addition reaction through EDC and NHS by adopting the property of carboxyl functional groups on the GQDs surface in the electrode modification process, thereby forming the stacked structure of DNA-GQDs-DNA. In comparison with original DNA / Au electrodes, modified DNA / GQDs / DNA / Au electrodes are excellent in conductivity and electrochemical performances, and can be used for measuring gene segments of Micro RNA and other specific sequences. The electrode modification method has the advantages that the manufacturing process is simple, the manufacturing cost is low, and besides, the electrodes can be conserved for a longer time in air, and thus the method is conveniently applied to research and actual detection.

Description

technical field [0001] The invention relates to a new biosensor method for orderly assembling DNA and graphene quantum dots on the surface of a gold electrode through chemical bonding, and belongs to the field of electrochemical biosensors. Background technique [0002] Graphene, as a new type of material with a single-layer sheet structure composed of carbon atoms in sp2 hybridized orbitals and a planar film with a hexagonal honeycomb lattice, has been widely used since its discovery due to its unique physical and chemical properties. research hotspot. Because of its excellent thermal conductivity and high electron transfer rate, graphene is an ideal material for modifying electrode surfaces. In recent years, with the development of electrochemical detection technology, many methods of graphene and its derivatives modified electrodes have emerged, such as electrochemical reduction of graphene oxide modified glassy carbon electrodes, carboxylated graphene modified glassy ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 鲁理平郭林青
Owner BEIJING UNIV OF TECH
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