Protein with similar function of irisin structural domain, and composition comprising protein and application of protein

An irisin and domain technology, applied in the field of multifunctional proteins, to achieve the effects of reducing body stress, obtaining convenience, and treating obesity

Inactive Publication Date: 2015-06-24
戴安
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is no application of recombinant irisin or its functional ...

Method used

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  • Protein with similar function of irisin structural domain, and composition comprising protein and application of protein
  • Protein with similar function of irisin structural domain, and composition comprising protein and application of protein
  • Protein with similar function of irisin structural domain, and composition comprising protein and application of protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Detection of human irisin A in rat islet cells and skin cells

[0037] experimental method:

[0038] The DNA sequence corresponding to the sequence of human irisin A (SEQ ID NO.1) was optimized according to the codon preference of Pichia pastoris, and then constructed into pGAPZαA plasmid. Transform into yeast competent cells by electric shock, and screen to obtain genetically stable strains. After the bacterial strain was inoculated into the optimized medium at a ratio of 5% (V / V), it was fermented at a high density for 72 hours, the supernatant of the fermentation broth was separated by centrifugation, and the supernatant was purified by a Ni column to obtain human irisin A. The preliminarily purified protein was purified by FPLC to collect the purified protein with a complete sequence detection peak. Mix it with normal saline at an appropriate dilution ratio gradient, inject intramuscularly into female 4-week-old Wistar rats, inject once every 48 hours, ...

Embodiment 2

[0041] Example 2: Human irisin A detection of lipogenic gene and brown fat conversion gene expression in rats

[0042] experimental method:

[0043] The DNA sequence corresponding to the sequence of human irisin A (SEQ ID NO.1) was optimized according to the codon preference of Pichia pastoris, and then constructed into pGAPZαA plasmid. Transform into yeast competent cells by electric shock, and screen to obtain genetically stable strains. After the bacterial strain was inoculated into the optimized medium at a ratio of 5% (V / V), it was fermented at a high density for 72 hours, the supernatant of the fermentation broth was separated by centrifugation, and the supernatant was purified by a Ni column to obtain human irisin A. The preliminarily purified protein was purified by FPLC to collect the purified protein with a complete sequence detection peak. Mix it with normal saline at an appropriate dilution ratio gradient, and inject intramuscularly into female 4-week-old Wistar ...

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Abstract

The invention provides a protein with a similar function of an irisin structural domain. The protein is a brand-new protein, is simple in structure, convenient to obtain, and is capable of rapidly carrying out mutation transformation. An experiment proves that the protein has similar characteristic of the irisin structural domain, and is capable of transforming white fat in a body into brown fat to cause energy consumption and immunity enhancement, so that a new effective way is provided for prevention or treatment of diseases or states caused by excessive energy intake/storage; the protein has the major performances that white fat accumulation in the body is reduced; the blood glucose and insulin level is regulated and balanced; the pressure of the organism is relieved, so as to reach the efficacy of treating obesity; hypomnesia caused by senile and organic neural function degradation is improved; the memory ability is improved; the energy storage level in the body is reduced; the telomere shortening speed is retarded, so as to retard organism ageing. The protein provided by the invention has safe and efficient favorable conditions on regulation of a natural signal channel.

Description

technical field [0001] The present invention relates to a multifunctional protein, in particular to a protein having a similar function to an irisin domain, a composition comprising the protein and an application thereof. Background technique [0002] Irisin (irisin) is a protein containing 112 amino acid residues isolated by Bostrom and colleagues in 2012, and its molecular weight is about 12kDa. Bostrom states that irisin is a glycoprotein and that its physiologically active state is affected by varying degrees of glycosylation. Irisin is the product of the cleavage of type III fibronectin domain protein 5 (FNDC5) by an unknown protease in human muscle tissue. FNDC5 is an in vivo protein discovered in 2002. Murine FNDC5 contains three parts: a signal peptide consisting of 29 amino acid residues; a type III fibronectin domain of 94 amino acid residues and a C-terminal. Transmembrane FNDC5 is slightly larger than free FNDC5 at approximately 32 kDa. Irisin is the C-termina...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12N15/12A61K38/39A61K8/64A61P3/04A23L1/29A23L33/00
CPCC07K14/78A23L33/17A23V2002/00A61K8/64A61K38/00A23V2200/30A23V2200/332A23V2250/542
Inventor 赵伟李波
Owner 戴安
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