Production process of effectively extracting heparin sodium

A production process and a technology for heparin sodium, which are applied in the field of production processes for efficiently extracting heparin sodium, can solve the problems of low yield of heparin sodium, cumbersome work, and high cost, and achieve the effects of ensuring high activity, ensuring titer, and maintaining titer.

Inactive Publication Date: 2015-06-24
ANHUI BAODI MEAT FOODS
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the heparin sodium produced by this process has low yield and low potency, and the acquisition of high...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The present invention will be further described below in conjunction with embodiment:

[0023] A production process for efficiently extracting heparin sodium, comprising the steps of:

[0024] 1. Material pretreatment

[0025] Fresh porcine small intestines were rinsed, cut open, and the intestinal mucosa was scraped with a razor blade, collected into ziplock bags, and stored at 4°C for later use.

[0026] 2. Enzymolysis

[0027] Dilute 50 kg of intestinal mucosa collected in step 1 with water until the Baume degree is 8-9 (measured by Baume meter, 4mol / L sodium chloride to adjust the concentration), adjust the pH to 8.0-10.0, heat and boil for denaturation for 15 minutes, and wait for the temperature to drop. When the temperature reaches about 50-55°C, add 1% (m / v) protein compound enzyme, incubate for 2 hours for enzymatic hydrolysis, centrifuge the enzymatic solution at 4°C, 4000r / min for 15min, and collect the supernatant.

[0028] 3. Resin adsorption

[0029] A...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a production process of effectively extracting heparin sodium. According to the production process, with the valence of a heparin sodium final product as an index and porcine intestinal mucosa as a raw material, heparin sodium (anticoagulant drug) with high valence is extracted through a production process method comprising the steps of enzymolysis, resin adsorption/elution, membrane separation, ethanol precipitation and drying. Compared with the traditional method comprising the steps of salt resolving, ion exchange and ethanol precipitation, the method disclosed by the invention has the advantages that an enzymolysis and membrane separation technology is effectively combined with a precipitation technology so that the valence of the extracted heparin sodium can be effectively increased to 160 U/mg and improved by nearly 64 percent, and the final recovery rate is improved by 29 percent. Meanwhile, a subsequent refining process can be shortened, the production cost is reduced, and the separation yield and the separation efficiency of heparin sodium can be improved.

Description

(1) Technical field [0001] The invention relates to a method for efficiently extracting heparin sodium, in particular to a production process for efficiently extracting heparin sodium by utilizing an enzyme method in combination with membrane separation technology and precipitation technology. (2) Background technology [0002] Heparin is an acidic mucopolysaccharide composed of D-glucosamine, D-glucuronic acid, N-acetylglucosamine and L-iduronic acid, widely present in mammalian intestinal mucosa, heart , liver, lung, blood, pancreas, and placenta. It has good anticoagulant, hypolipidemic, fibrinolytic and anti-medial smooth muscle cell proliferation activities, and is widely used in the treatment of cardiovascular and cerebrovascular diseases. Heparin sodium is the sodium salt form of heparin, which is prepared in the form of sodium salt during the extraction process of heparin, which is convenient for the preservation of drug efficacy and activity, and can be used as a p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/10
Inventor 董浩张秀民罗志良符绍辉程榆茗夏毅强高振宏
Owner ANHUI BAODI MEAT FOODS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap