Preparation method of bacterial cell catalyst for catalyzing naringin ester synthesis reaction

A bacterial cell and synthesis reaction technology, applied in the field of bacterial cell catalyst preparation, can solve the problems of low product purity, low substrate utilization, low selectivity, etc., and achieves high regioselectivity, mild reaction conditions, and low preparation cost. Effect

Inactive Publication Date: 2015-06-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The cell catalyst that can catalyze the synthesis reaction of naringin ester prepared by this method can selectively catalyze the synthesis of naringin 6''-monoester, and its regioselectivity reaches more than 97%, which overcomes the low selectivity of traditional chemical methods Lead to low substrate utilization, low product purity, easy to generate by-products and other shortcomings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] will include Pseudomonas aeruginosa Pseudomonas aeruginosa GIM1.46 The seed liquid was transferred to a liquid medium for culture, the inoculation amount was 0.5% (v / v), the culture condition was 30°C, and the liquid medium composition was: 0.5 g / L soybean oil, 0.5 g / L yeast extract, 0.5 g / L MgSO 4 ·7H 2 O , 5 g / L (NH 4 ) 2 SO 4 , 1 g / L K 2 HPO 4 , pH 7.0. After culturing for 60 hours, the wet cells were collected by centrifugation, washed twice with distilled water, and vacuum freeze-dried to obtain the cell catalyst. The cellular catalyst was used in the acylation reaction of naringin and vinyl propionate, and the regioselectivity of 6''-naringin propyl ester was 98.7%.

[0020] The regioselectivity of the catalyst obtained by the method of the present invention is determined by the regioselective acylation reaction of naringin and vinyl propionate, and the reaction condition is to add 80 mg of bacterium dry powder in the naringin esterification reaction syst...

Embodiment 2

[0022] will include Pseudomonas aeruginosa Pseudomonas aeruginosa GIM1.46 The seed solution was transferred to a liquid medium for culture, the inoculation amount was 0.5% (v / v), the culture condition was 50°C, and the liquid medium composition was: 0.5 g / L Tween 80, 0.5 g / L yeast extract, 0.5 g / L MgSO 4 ·7H 2 O , 5 g / L (NH 4 ) 2 SO 4 , 1 g / L K 2 HPO 4 , pH 7.0. After culturing for 60 hours, the wet cells were collected by centrifugation, washed twice with distilled water, and vacuum freeze-dried to obtain the cell catalyst. The cellular catalyst was used in the acylation reaction of naringin and vinyl propionate, and the regioselectivity of 6''-naringin propyl ester was 97.7%.

Embodiment 3

[0024] will include Pseudomonas aeruginosa Pseudomonas aeruginosa GIM1.46 The seed liquid was transferred to a liquid medium for culture, the inoculum size was 20% (v / v), the culture condition was 30°C, and the liquid medium composition was: 50 g / L Tween 80, 10 g / L yeast extract, 0.2 g / L MgSO 4 ·7H 2 O , 5 g / L (NH 4 ) 2 SO 4 , 1 g / L K 2 HPO 4 , pH 6.8. After culturing for 48 hours, the wet cells were collected by centrifugation, washed twice with distilled water, and vacuum freeze-dried to obtain the cell catalyst. The cell catalyst was used in the acylation reaction of naringin and vinyl propionate, and the regioselectivity of 6''-naringin propyl ester was 98.2%.

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Abstract

The invention discloses a preparation method of a bacterial cell catalyst for catalyzing naringin ester synthesis reaction. The method comprises the following steps: inoculating a bacterium seed solution into an enzyme-inducer-containing liquid culture medium, and culturing at 30-50 DEG C for 12-144 hours, wherein the inoculum size is 0.5-20% (v / v); and centrifuging, collecting the thallus, washing with distilled water twice, carrying out vacuum freeze drying for 12-36 hours, and collecting the thallus dry powder to obtain the bacterial cell catalyst. The catalyst is used for catalyzing ester conversion reaction between esculine and an acyl reagent at high selectivity. Compared with the enzyme preparation, the obtained bacterial full-cell catalyst avoids the expensive and complex enzyme separation purification process, is easy to prepare and recover, is more beneficial to large-scale production, and has higher stability in organic solvents.

Description

technical field [0001] The invention belongs to the fields of microorganism cultivation, biocatalysis and biomedicine preparation, and in particular relates to a preparation method of a bacterial cell catalyst for catalyzing the synthesis reaction of naringin ester. Background technique [0002] Flavonoids are a series of important polyphenolic compounds formed by connecting two benzene rings through three carbon atoms. They are widely found in almost all plants, especially vegetables and fruits; they are widely used in medicine, food and cosmetics. Great application value. Flavonoids have a variety of biological activities including antioxidant activity, anti-inflammatory, anticancer, antibacterial, antiallergic, and antiviral, etc., however, most of the biological activities of flavonoids are limited by their low lipid solubility due to their polyhydroxy structure. or water soluble. The hydroxyl group located on the benzene ring or the glycoside skeleton has an important...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/38C12R1/385
CPCC12N1/20
Inventor 李晓凤赖学能赵光磊吴晖余以刚
Owner SOUTH CHINA UNIV OF TECH
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