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Amplification culture method for producing ethanol microorganisms by virtue of co-fermentation of C5 and C6

A technology for expanding the cultivation of microorganisms, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as difficulty in realizing large-scale industrial application, low vitality, and slow growth and proliferation of microorganisms, and achieve beneficial Effects of rapid proliferation, enhanced fermentation activity, and increased yield

Inactive Publication Date: 2015-07-01
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Therefore, the object of the present invention is to overcome the defects in the prior art that the expansion and proliferation of microorganisms is slow, the vitality is low, and it is difficult to realize large-scale industrial application, thereby providing a method for expanding the cultivation of microorganisms that co-ferment C5 and C6 to produce ethanol

Method used

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  • Amplification culture method for producing ethanol microorganisms by virtue of co-fermentation of C5 and C6

Examples

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Embodiment 1

[0051] This example provides an expanded culture method for microorganisms that co-ferment C5 and C6 to produce ethanol. Taking recombinant S. -ST) (such as Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A (LNH-ST), M.W. Lau, B.E. Dale, Proc Natl Acad Sci USA, 106 (2009), pp. 1368-1373; Two-step SSCF to convert AFEX-treated switchgrass to ethanol using commercial enzymes and Saccharomyces cerevisiae 424A(LNH-ST), M. Jin, M.W. Lau, V. Balan, B.E. Dale, Bioresour Technol, 101 (2010), pp. 8171–8178, etc. report), which is commercially available, and the expansion cultivation method adopts a first-level expansion cultivation step, which specifically includes:

[0052] A. Seed liquid culture steps

[0053] Prepare YEPD medium, inoculate the above-mentioned preserved bacteria into YEPD medium, control the temperature at 25°C, pH6.0, rotation speed 200rpm, ventilation volume 0.2vvm, and cultivate for 12h until the concentration of the a...

Embodiment 2

[0067] This example provides an expanded culture method for microorganisms that co-ferment C5 and C6 to produce ethanol. Taking Candida tropicalis as an example to carry out the expanded culture experiment, the Candida tropicalis is commercially available, and the expanded culture The method adopts two-stage expanded culture steps, which specifically include:

[0068] A. Seed liquid culture steps

[0069] Prepare YEPD medium, inoculate the above-mentioned preserved bacteria into YEPD medium, control the temperature at 25°C, pH6.0, rotating speed at 200rpm, and ventilation at 0.2vvm, and cultivate until the concentration of the above-mentioned bacteria is (0.1-0.5)×10 9 / ml;

[0070] The YEPD medium specifically contains 10 g / L of yeast powder, 20 g / L of peptone, and 20 g / L of glucose, and the pH is adjusted to 6.0.

[0071] B. Expand the cultivation steps

[0072] 1) Put the cultured seed solution into a 50L primary expansion tank containing a primary expansion medium at an...

Embodiment 3

[0085] This example provides an expanded culture method for microorganisms that co-ferment C5 and C6 to produce ethanol. Taking recombinant Saccharomyces cerevisiae 424A (LNH-ST) as an example to carry out the expanded culture experiment (same as Example 1), the expanded culture method Adopt three-level expansion culture steps, including:

[0086] A. Seed liquid culture steps

[0087] Prepare YEPD medium, inoculate the above-mentioned preserved bacteria into YEPD medium, control the temperature at 30°C, pH6.0, ventilation rate 0.2vvm, and rotation speed 200rpm, and cultivate for 14h until the concentration of the above-mentioned bacteria is (0.2-0.3)× 10 9 / ml;

[0088] The YEPD medium specifically contains 5 g / L of yeast powder, 1 g / L of peptone, and 25 g / L of glucose, and the pH is adjusted to 6.5.

[0089] B. Expand the cultivation steps

[0090] 1) Put the cultured seed liquid into a 50L primary expansion tank containing a primary expansion medium at an inoculation amo...

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Abstract

The invention provides an amplification culture method for producing ethanol microorganisms by virtue of co-fermentation of C5 and C6. The method comprises the following steps: inoculating a seed solution obtained by culturing the microorganisms to an amplification culture medium; performing amplification culture on the microorganisms by virtue of at least one stage of an amplification culture step; and adjusting the oxygen supply concentration in the process of amplification culture to realize the amplification culture of the microorganisms.

Description

technical field [0001] The invention relates to an enlarged culture method for co-fermenting C5 and C6 microorganisms to produce ethanol, and belongs to the technical field of microbial fermentation. Background technique [0002] The depletion of many non-renewable fossil energy sources such as oil has drawn more and more attention to renewable energy, especially biofuels, and has brought huge business opportunities and social significance. [0003] According to the relevant definitions of the International Energy Agency (IEA), conventional biofuels include sugar-based and starch-based ethanol, oil crop-based biodiesel, directly usable vegetable oils, and biogas produced by anaerobic digestion; Transformation technologies in the R&D, pilot or demonstration stages are usually referred to as second-generation technologies or third-generation technologies. This category includes hydrogenated vegetable oils (HVO) produced from animal fats and vegetable oils, as well as biofuels...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/18C12N1/14C12P7/06C12R1/865C12R1/19C12R1/01C12R1/645C12R1/84C12R1/72C12R1/74C12R1/18C12R1/22C12R1/145C12R1/77
CPCC12N1/14C12N1/16C12N1/18C12N1/20C12P7/06Y02E50/10
Inventor 李凡沈乃东林鑫苏会波彭超熊强林海龙李春玲袁敬伟刘文信岳国君
Owner COFCO NUTRITION & HEALTH RES INST
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