Preparation method of novel anticoagulant stents coating capable of capturing endothelial progenitor cells

A technology of endothelial progenitor cells and anticoagulation, applied in the field of biomedical engineering materials, can solve the problems of poor anticoagulant performance and blood compatibility, achieve excellent anticoagulant ability, promote rapid endothelialization, and reduce the risk of late thrombus Effect

Inactive Publication Date: 2015-07-08
SOUTHWEST JIAOTONG UNIV
View PDF7 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in most cases, while endothelialization is often promoted, the anticoagulant performance and blood compatibility of the implanted materia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of novel anticoagulant stents coating capable of capturing endothelial progenitor cells
  • Preparation method of novel anticoagulant stents coating capable of capturing endothelial progenitor cells
  • Preparation method of novel anticoagulant stents coating capable of capturing endothelial progenitor cells

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] QCM-D implementation plan 2

[0033] 1. Prepare the sample grafted with CD133 using gold flakes;

[0034] 2. Then use SDS and UP water to clean the QCM-D channel in turn;

[0035] 3. Place the sample, then open the software, set the temperature to 37℃, and detect the sensitivity of the instrument;

[0036] 4. Start to run the air, check whether the QCM-D sequins are intact; then pass in PBS buffer

[0037] 5. After the baseline runs flat, pass the αMEM (containing 10% bovine serum albumin) medium solution for about 30 minutes to obtain a more plateau baseline;

[0038] 6. Pass the EPCs cell solution containing bovine serum albumin (cell density is about 10^4 / ml); finally wait for about 8 hours, then observe the changes in frequency and dissipation, the experiment is over, and the data is analyzed.

Example Embodiment

[0039] Example 1

[0040] 1. Wash the titanium oxide stent with SDS, alcohol (ethanol), RO water (deionized water) ultrasonically three times for 10 minutes each time, and then dry it;

[0041] 2. Hydroxylation treatment of the glass slide: Put the glass slide into concentrated sulfuric acid and hydrogen peroxide in a volume ratio of 7:3 (V%:V%=7:3) and soak in 100℃ for 2h; then rinse with RO water 3 Times, 5min each time;

[0042] 3. Then immediately put the above-mentioned sample into the Tris-base (10mM, pH8.5) buffer solution, put it in a shaker, seal and shake for about 1h, and then open and shake for about 11h. Then use RO water ultrasonic cleaning 3 times, and dry it with cold air.

[0043] 4. Then configure 10ml of EDC / NHS / MES (1omM / 20mM / 50mM) PBS solution with pH 5.4, and then dilute the CD133 solution with EDC to 0.8-3.2ug / ml and Fucoidan concentration 50-400ug / ml; it will be prepared Add 50ul each of CD133 solution and Fucoidan dropwise to the surface of the glass slide; ...

Example Embodiment

[0044] Example 2

[0045] 1. Wash the titanium oxide stent with SDS, alcohol (ethanol), RO water (deionized water) ultrasonically three times for 10 minutes each time, and then dry it;

[0046] 2. Hydroxylation treatment of the glass slide: Put the glass slide into concentrated sulfuric acid and hydrogen peroxide in a volume ratio of 7:3 (V%:V%=7:3) and soak in 100℃ for 2h; then rinse with RO water 3 Times, 5min each time;

[0047] 3. Then immediately put the above sample in the Tris-base (10mM, pH8.5) buffer solution, soak for 8h, and then ultrasonically clean with RO water 3 times, 5min each time, and then continue to soak for 3 times in a row. Sealed storage of dopamine film slides;

[0048] 4. Then configure 10ml of EDC / NHS / MES (5mM / 10mM / 50mM) PBS solution with pH7.4, and then use EDC to dilute the CD133 solution concentration to 0.8-3.2ug / ml and Fucoidan concentration 50-400ug / ml; Add 50ul each of CD133 solution and Fucoidan dropwise to the surface of the glass slide; react for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of novel anticoagulant stents coating capable of capturing endothelial progenitor cells, which comprises the following steps: performing hydroxylation processing on surface of a material, then dissolving dopamine in a Tris-base(pH is 8.5, 2mg/ml) solution, then depositing a layer or multilayer dopamine membrane at temperature of 37 DEG C on the surface of the material, then adjusting reaction condition according to pH and EDC/NHS concentration, and performing covalent grafting on a mixture of CD133+(0.8-3.2ug/ml) and Fucoidan(50-400ug/ml) at temperature of 37 DEG C to obtain a target product. The preparation method has the advantages of simple operation, mild reaction condition, high efficiency and low cost. A target coating has strong antithrombotic performance, anticoagulation and inhibition of endometrial hyperplasia of smooth muscle due to effect of low molecular weight Fucoidan, can rapidly capture the endothelial progenitor cells of peripheral blood so as to induce the endothelial progenitor cells to differentiate for obtaining endothelial cells, and then a complete endothelial cells layer is formed, and the novel anticoagulant stents coating has excellent anticoagulation performance and biological compatibility.

Description

technical field [0001] The invention relates to a biomedical engineering material, especially a multifunctional coating for capturing stem cells and endothelial progenitor cells EPCs with good blood compatibility. Background technique [0002] According to the WHO report, cardiovascular disease has become the main killer of people's death. PCI stent interventional surgery is a relatively mature and effective method, which can greatly relieve people's suffering and reduce mortality. However, after interventional surgery, restenosis and late thrombosis still have a rate of up to 30% 3-6 months after stent intervention, which is mainly due to the lack of biocompatibility and functionality of the stent surface, which cannot be quickly treated. Formation of a complete and continuous endothelial layer. The inner layer of blood vessels is mainly composed of a single layer of endothelial cells and basement membrane, and the middle layer is mainly composed of smooth muscle cells. Th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L33/08A61L31/08
Inventor 赵安莎薛国能黄楠杨苹王艳
Owner SOUTHWEST JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap