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Preparation method of novel anticoagulant stents coating capable of capturing endothelial progenitor cells

A technology of endothelial progenitor cells and anticoagulation, applied in the field of biomedical engineering materials, can solve the problems of poor anticoagulant performance and blood compatibility, achieve excellent anticoagulant ability, promote rapid endothelialization, and reduce the risk of late thrombus Effect

Inactive Publication Date: 2015-07-08
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in most cases, while endothelialization is often promoted, the anticoagulant performance and blood compatibility of the implanted material surface are poor, such as extracellular matrix proteins, CD133, etc., so we also need to co-graft a Biomolecules with excellent biocompatibility

Method used

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  • Preparation method of novel anticoagulant stents coating capable of capturing endothelial progenitor cells
  • Preparation method of novel anticoagulant stents coating capable of capturing endothelial progenitor cells
  • Preparation method of novel anticoagulant stents coating capable of capturing endothelial progenitor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 2

[0033] 1. Prepare a sample grafted with CD133 using a gold sheet;

[0034] 2. Then wash the QCM-D channel with SDS and UP water in sequence;

[0035] 3. Place the sample, then open the software, set the temperature to 37°C, and test the sensitivity of the instrument;

[0036] 4. Start to run the air to check whether the QCM-D gold sheet is intact; then pass into the PBS buffer

[0037] 5. After the baseline runs flat, inject αMEM (containing 10% bovine serum albumin) medium solution for about 30 minutes to obtain a relatively flat baseline;

[0038] 6. Pass through the EPCs cell solution containing bovine serum albumin (the cell density is about 10^4 / ml); finally wait for about 8 hours, and then observe the changes in frequency and dissipation. After the experiment is over, analyze the data.

Embodiment 1

[0040] 1. The titanium oxide stent was ultrasonically cleaned three times with SDS, alcohol (ethanol), and RO water (deionized water) for 10 minutes each time, and then dried;

[0041] 2. Hydroxylation treatment of slides: Put the slides in concentrated sulfuric acid and hydrogen peroxide according to the volume ratio of 7:3 (V%:V%=7:3) and soak in 100°C for 2 hours; then wash with RO water for 3 hours times, 5 minutes each time;

[0042] 3. Then immediately put the above sample into Tris-base (10mM, pH8.5) buffer solution, put it in a shaker and shake it tightly for about 1 hour, and then shake it open for about 11 hours. Then use RO water to ultrasonically clean 3 times, and dry with cold air.

[0043] 4. Then configure 10ml of EDC / NHS / MES (1omM / 20mM / 50mM) PBS solution with pH 5.4, then dilute the concentration of CD133 solution with EDC to 0.8-3.2ug / ml and the concentration of Fucoidan to 50-400ug / ml; 50ul of CD133 solution and Fucoidan were dropped onto the surface of th...

Embodiment 2

[0045] 1. The titanium oxide stent was ultrasonically cleaned three times with SDS, alcohol (ethanol), and RO water (deionized water) for 10 minutes each time, and then dried;

[0046] 2. Hydroxylation treatment of slides: Put the slides in concentrated sulfuric acid and hydrogen peroxide according to the volume ratio of 7:3 (V%:V%=7:3) and soak in 100°C for 2 hours; then wash with RO water for 3 hours times, 5 minutes each time;

[0047] 3. Immediately put the above sample into Tris-base (10mM, pH8.5) buffer solution, soak for 8h, then ultrasonically clean it with RO water for 3 times, each time for 5min, and then continue to soak for 3 times in a row. The glass slides of the dopamine film are sealed and preserved;

[0048] 4. Then configure 10ml of EDC / NHS / MES (5mM / 10mM / 50mM) PBS solution with pH 7.4, then dilute the concentration of CD133 solution with EDC to 0.8-3.2ug / ml and the concentration of Fucoidan to 50-400ug / ml; 50ul of CD133 solution and Fucoidan were dropped on...

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Abstract

The invention discloses a preparation method of novel anticoagulant stents coating capable of capturing endothelial progenitor cells, which comprises the following steps: performing hydroxylation processing on surface of a material, then dissolving dopamine in a Tris-base(pH is 8.5, 2mg / ml) solution, then depositing a layer or multilayer dopamine membrane at temperature of 37 DEG C on the surface of the material, then adjusting reaction condition according to pH and EDC / NHS concentration, and performing covalent grafting on a mixture of CD133+(0.8-3.2ug / ml) and Fucoidan(50-400ug / ml) at temperature of 37 DEG C to obtain a target product. The preparation method has the advantages of simple operation, mild reaction condition, high efficiency and low cost. A target coating has strong antithrombotic performance, anticoagulation and inhibition of endometrial hyperplasia of smooth muscle due to effect of low molecular weight Fucoidan, can rapidly capture the endothelial progenitor cells of peripheral blood so as to induce the endothelial progenitor cells to differentiate for obtaining endothelial cells, and then a complete endothelial cells layer is formed, and the novel anticoagulant stents coating has excellent anticoagulation performance and biological compatibility.

Description

technical field [0001] The invention relates to a biomedical engineering material, especially a multifunctional coating for capturing stem cells and endothelial progenitor cells EPCs with good blood compatibility. Background technique [0002] According to the WHO report, cardiovascular disease has become the main killer of people's death. PCI stent interventional surgery is a relatively mature and effective method, which can greatly relieve people's suffering and reduce mortality. However, after interventional surgery, restenosis and late thrombosis still have a rate of up to 30% 3-6 months after stent intervention, which is mainly due to the lack of biocompatibility and functionality of the stent surface, which cannot be quickly treated. Formation of a complete and continuous endothelial layer. The inner layer of blood vessels is mainly composed of a single layer of endothelial cells and basement membrane, and the middle layer is mainly composed of smooth muscle cells. Th...

Claims

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Application Information

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IPC IPC(8): A61L33/08A61L31/08
Inventor 赵安莎薛国能黄楠杨苹王艳
Owner SOUTHWEST JIAOTONG UNIV
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