Polymyxin B production fermentation culture medium, preparation method and uses thereof

Abstract

The present invention discloses a polymyxin B production fermentation culture medium, a preparation method and uses thereof, wherein the culture medium comprises, by weight, 0.1-1.5 w / w% of an organic nitrogen source, 5.0-15.0 w / w% of whole wheat powder, and 0.3-1.2 w / w% of an inorganic salt.

Description

technical field

[0001] The invention relates to the field of biotechnology; in particular, it relates to a fermentation medium and its preparation method and application. Background technique

[0002] Polymyxin B (Polymyxin B, PMB) is a basic cyclic peptide antibiotic produced by Bacillus polymyxa or Paenibacillus polymyxa, which has good activity against Gram-negative bacteria. Colistin B drugs include polymyxin B sulfate injection and compound topical preparations of polymyxin B sulfate and other antibiotics, which are used to treat skin wounds, peritonitis, urinary system and respiratory system caused by Gram-negative bacteria Infection at other sites; PMB can also significantly inhibit the acute phase response induced by endotoxin, so it can also be used for sepsis.

[0003] At present, the preparation of polymyxin B adopts the fermentation method. After the seeds of Bacillus polymyxa or Paenibacillus polymyxa are cultured, they are inoculated in a fermenter and cultiva...

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