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Phellinus linteus polysaccharide separation and purification method

A technology for separation and purification of Phellinus spp., which is applied in the direction of drug combination and antineoplastic drugs, and can solve the problems that limit the deep development and application of active polysaccharides of Phellinus spp., and the separation and purification methods have not been reported yet.

Inactive Publication Date: 2015-08-12
JIANGSU UNIV +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nevertheless, the anti-tumor effect of Phellinus polysaccharides at home and abroad is mainly researched on water extracts or water-extracted polysaccharides. For polysaccharides prepared from other extraction media such as acid and lye, there are still few domestic and foreign studies, which are limited. The in-depth development and application of Phellinus active polysaccharides
[0003] After retrieval, there is no report on the separation and purification method of Phellinus souriflorum polysaccharide

Method used

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  • Phellinus linteus polysaccharide separation and purification method
  • Phellinus linteus polysaccharide separation and purification method
  • Phellinus linteus polysaccharide separation and purification method

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Embodiment 1

[0024] 1. Degreasing: crush the Phellinus mycelium, wrap it in absorbent cotton cloth, put it in a 500 mL Soxhlet extractor, add 200~300 mL petroleum ether (boiling range: 30~60°C), reflux at 35°C for 6 h, repeat Degrease once and let it dry naturally.

[0025] 2. Acid extraction: 100 g of the degreased Phellinus mycelium obtained in step 1 was added to 1000 mL of ammonium oxalate solution with a mass fraction of 1% at a solid-liquid ratio of 1:10 (g / mL), and placed in a 2000 mL round bottom In the flask, stir well and reflux at 95°C for 8 h. The mixture was centrifuged in a 300 mL plastic centrifuge tube at 5000 rpm for 20 min, and the supernatant was combined and evaporated on a 45°C rotary evaporator under reduced pressure, concentrated to 1.5 times the original volume, added 4 times the volume of 95% ethanol, and left in a refrigerator at 4°C Overnight, the precipitate was collected by centrifugation, and the crude polysaccharide of Phellinus souriflorus was obtained. Re...

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Abstract

The invention discloses a Phellinus linteus polysaccharide separation and purification method which is characterized by mainly comprising the following steps: preparing a Phellinus linteus polysaccharide component by an ammonium oxalate extraction-ethanol precipitation process, carrying out anion exchange chromatography and gel filtration chromatography to separate and purify the polysaccharide, and carrying out concentration, dialysis, vacuum freeze drying and the like on the product to obtain the Phellinus linteus polysaccharide pure product. The method does not influence the chemical structure of the natural polysaccharide, and can keep the original bioactivity of the natural polysaccharide. The method has the advantages of low requirements for equipment and environment, simple process and low cost, is flexible and practical, and is beneficial to popularization, development and application.

Description

technical field [0001] The invention relates to a method for separating and purifying the Phellinus polysaccharide from Phellinus mycelium by applying bioengineering technology. Background technique [0002] Phellinus ( Phellinus linteus ) belongs to Basidiomycotina, Polyporaceae, Xylinophora ( Phellinus ), is a precious medicinal fungus, known as "forest gold". Studies have found that Phellinus has a significant anti-tumor effect, and its inhibitory effect on S180 and gastric cancer is stronger than that of medicinal fungi such as Ganoderma lucidum and Agaricus mushroom. It is currently internationally recognized as the most effective medicinal fungus in the field of biological cancer treatment , has the potential to develop natural anti-tumor biological products in which polysaccharides are the main active ingredients. Nevertheless, the anti-tumor effect of Phellinus polysaccharides at home and abroad is mainly researched on water extracts or water-extracted polysaccha...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
Inventor 闫景坤王振斌夏蓉裴娟娟陶汇源马海乐崔凤杰杨小明庄俊茹
Owner JIANGSU UNIV
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