A kind of preparation method of liquid nisin preservative

A preservative and liquid technology, applied in the field of bioengineering, can solve the problems of low extraction yield, insufficient market competitiveness, and high cost, and achieve the effects of easy mixing, outstanding antiseptic effect, and convenient use

Active Publication Date: 2018-07-27
SHANDONG FREDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems of low extraction yield, high cost and insufficient market competitiveness in the existing nisin production process. After pretreatment, ceramic membrane filtration sterilization is carried out. After sterilization, the clear liquid is concentrated by foam separation to obtain a high-potency nisin solution. After adding protective agents and stabilizers in proportion, a liquid Nisin preservative is obtained.

Method used

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  • A kind of preparation method of liquid nisin preservative
  • A kind of preparation method of liquid nisin preservative
  • A kind of preparation method of liquid nisin preservative

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Fermentation broth pretreatment, ceramic membrane membrane filtration to remove impurities such as bacteria and macromolecular proteins, to obtain the fermented liquid of Nisin.

[0027] (2) Foam separation and concentration, feed air into the foam separation device, control the air flow rate to 3-6L / h, and collect the foam liquid.

[0028] (3) Foam defoaming treatment, utilizing the principle of physical defoaming, combined with adding polyoxyethylene polypropylene glyceryl ether for defoaming treatment, to obtain a high-titer Nisin solution (100000IU / mL).

[0029] (4) Adding protective agent, start stirring, add 2% sodium chloride, 0.5% sucrose, 0.3% sodium glutamate, 0.6% maltodextrin respectively, and stir evenly to dissolve various protective agents completely. The amount of addition is based on the weight of the high titer Nisin solution in step (3).

[0030] (5) Adding stabilizers, start stirring, add 0.8% sorbitol, 0.4% microcrystalline cellulose, 0.0003% ...

Embodiment 2

[0032] (1) Fermentation broth pretreatment, ceramic membrane membrane filtration to remove impurities such as bacteria and macromolecular proteins, to obtain the fermented liquid of Nisin.

[0033] (2) Foam separation and concentration, pass carbon dioxide and air into the foam separation device, the ratio is 0.1:1, control the air flow rate to 5-8L / h, and collect the foam liquid.

[0034] (3) Foam defoaming treatment, using the principle of physical defoaming, combined with adding edible oil for defoaming treatment, to obtain a high titer Nisin solution (100000IU / mL).

[0035] (4) Addition of protective agent, start stirring, add 1.8% sodium chloride, 0.6% sucrose, 0.2% sodium glutamate, 0.4% maltodextrin respectively, and stir evenly to dissolve various protective agents completely. The amount of addition is based on the weight of the high titer Nisin solution in step (3).

[0036](5) Adding stabilizer, start stirring, add 0.05% sorbitol, 0.02% microcrystalline cellulose, ...

Embodiment 3

[0038] (1) Fermentation broth pretreatment, ceramic membrane membrane filtration to remove impurities such as bacteria and macromolecular proteins, to obtain the fermented liquid of Nisin.

[0039] (2) Foam separation and concentration, pass carbon dioxide, air and nitrogen into the foam separation device, the ratio is 0.1:1:0.05, control the flow rate to 8-10L / h, and collect the foam liquid.

[0040] (3) Foam defoaming treatment, using the principle of physical defoaming, combined with adding edible oil for defoaming treatment, to obtain a high-potency Nisin solution with a potency of 100,000 IU / mL.

[0041] (4) Adding protective agent, start stirring, add 2% sodium chloride, 0.8% sucrose, 0.1% sodium glutamate, 0.5% maltodextrin respectively, and stir evenly to dissolve various protective agents completely. The amount of addition is based on the weight of the high titer Nisin solution in step (3).

[0042] (5) Adding stabilizer, start stirring, add 0.03% sorbitol, 0.015% m...

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Abstract

The invention discloses a preparation method of a liquid Nisin antiseptic and belongs to the field of bioengineering. The preparation method comprises pre-treating Nisin fermentation broth, carrying out filtration sterilization by a ceramic membrane, carrying out concentration on the clear liquid subjected to sterilization in a foam separation way so that a high-titer Nisin solution, and adding a protective agent and a stabilizing agent into the Nisin solution according to a certain ratio so that the liquid Nisin antiseptic is obtained. The liquid Nisin antiseptic has outstanding effects, is convenient for use, solves the problem of low yield of Nisin in extraction and is an antiseptic product with a good market prospect. Compared with the traditional antiseptic powder, the liquid antiseptic has outstanding performances, is convenient for use, simplifies a Nisin extraction step, reduces a loss late in extraction and reduces a production cost.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to a preparation method of liquid Nisin preservative. Background technique [0002] Nisin (Nisin) is a small molecular polypeptide compound composed of 34 amino acids produced by fermentation of Streptococcus lactis or some strains of Lactococcus lactis, with a molecular weight of about 3510, it can effectively inhibit the growth of Gram-positive bacteria and their spores that cause food spoilage, and prolong the shelf life of food. After FAO / WHO approved Nisin as a food additive in 1961, it has been used in more than 50 countries and countries around the world. The region approves it as a food preservative. In 1990, my country included it in the national standard GB2760-86, which can be used in canned food, vegetable protein food, dairy products and meat products. Since Nisin is easily degraded by some proteases in the human digestive tract and will not accumulate in the human body, it...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/315C07K1/14A23L3/3526
CPCA23L3/3526
Inventor 朱云峰苏移山赵文刚凌沛学袁艳华刘芳谢鲁光
Owner SHANDONG FREDA BIOTECH
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