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Disposable nanometer electrogenerated chemiluminescence two-component immune sensor and preparation method thereof

An immunosensor and electrochemistry technology, applied in chemiluminescence/bioluminescence, electrical excitation analysis, and analysis by chemical reaction of materials, etc., can solve problems such as complicated procedures, achieve simple preparation and operation process, and stable signal , the effect of low price

Inactive Publication Date: 2015-08-26
THE SECOND HOSPITAL OF NANJING
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  • Claims
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AI Technical Summary

Problems solved by technology

The currently widely used AFP-L3% detection method is lectin affinity column electrophoresis detection, which is relatively complicated and requires separate detection of AFP and AFP-L3
At the same time, the detection limit of the low-abundance protein AFP-L3 is limited by the detection limit of the methodology and instrument

Method used

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  • Disposable nanometer electrogenerated chemiluminescence two-component immune sensor and preparation method thereof
  • Disposable nanometer electrogenerated chemiluminescence two-component immune sensor and preparation method thereof
  • Disposable nanometer electrogenerated chemiluminescence two-component immune sensor and preparation method thereof

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Embodiment Construction

[0039] The two-component nano-ECL immunosensor based on two kinds of quantum dots (QDs) materials in the present invention is realized by using the QDs ECL potential difference resolution and the space resolution of the indium tin oxide (ITO) electrode wrapped by a single mercapto compound and a double mercapto compound. Simultaneous detection of two objects is a three-electrode structure, Ag / AgCl electrode is used as a reference electrode, Pt electrode is used as an auxiliary electrode, and two pieces of modified ITO conductive glass placed back to back to specifically recognize the two components are used as a two-component Working electrode of the immunosensor. Through the sandwich immunoreaction, the horseradish peroxidase (HRP) cross-linked to the surface of the ITO electrode after layer-by-layer assembly implements the enzymatic cycle signal amplification strategy. The specific steps are:

[0040] Step 1: Pass CdCl 2 Provides Cd source as precursor, NaBH 4 The NaHTe o...

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Abstract

The invention discloses a disposable nanometer electrogenerated chemiluminescence two-component immune sensor and a preparation method thereof. A monothiol compound and a bisthiol compound wrapped QDs electrogenerated chemiluminescence potential difference distinguish and ITO electrode space distinguish are used to achieve object detection. The sensor has a three-electrode structure; Ag / AgCl or saturated calomel electrode is used as a reference electrode, Pt electrode is used as an auxiliary electrode, and two pieces of modified ITO conductive glass capable of specifically identifying the two components are placed back to back as a working electrode for the two-component immune sensor; and an object to be measured is crosslinked to the surface of the working electrode by a sandwich immunoassay reaction, and finally crosslinked with horseradish peroxidase.

Description

technical field [0001] The invention relates to a two-component immunosensor based on electrochemiluminescence (ECL) signal tracing of two different shell quantum dots (quantum dots, QDs) and a preparation method thereof. The sensor detects the object to be measured based on the luminescence potential resolution of the two QDs materials and the spatial resolution of the ITO conductive glass. Background technique [0002] The ECL process refers to the electrochemical method of injecting electrons or holes into the luminophore or its co-reactant on the electrode surface, and the electron transfer occurs through the collision of the oxidized and reduced intermediates, thereby generating the excited state of the luminophore and transitioning back to the ground state. light radiation is produced. QDs, also known as nanocrystals, are composed of a limited number of atoms, and the three dimensions are all on the order of nanometers. QDs are generally spherical or quasi-spherical,...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N21/66G01N33/68
Inventor 刘璇姜晖赵伟王念跃
Owner THE SECOND HOSPITAL OF NANJING
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