Method for preparing feed raw material by using corn steep liquor as substrate and fermenting astragalus membranaceus with lactobacillus rhamnosus

A technology of Lactobacillus rhamnosus and corn steep liquor, applied in animal feed, animal feed, application, etc., can solve problems such as ineffectiveness, livestock and human hazards, avoid environmental pollution problems, have a good market prospect, and increase content Effect

Inactive Publication Date: 2015-09-02
HUBEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to improve the absorption rate of livestock to feed, some researchers add cellulolytic bacteria such as actinomycetes, but the effect is not significant; Harmful to livestock and humans

Method used

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  • Method for preparing feed raw material by using corn steep liquor as substrate and fermenting astragalus membranaceus with lactobacillus rhamnosus

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Embodiment 1

[0024] 1. Materials

[0025] (1) Strains: Lactobacillus rhamnosus.

[0026] (2) Seed medium: improved MRS medium, including 10g of peptone, 10g of beef extract, 5g of yeast extract, 20g of glucose, Tween-2080mL, K 2 HPO 4 2g, sodium acetate 5g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 4H 2 O 0.05g, distilled water 1000mL, pH 6.2-6.4, autoclave at 121°C for 15min.

[0027] (3) Fermentation medium raw materials: astragalus powder, corn steep liquor. Wherein, the preparation of Astragalus powder is as follows: Astragalus slices (purchased from Inner Mongolia Tianchuang Agriculture and Animal Husbandry Technology Co., Ltd.) are pulverized with a pulverizer, and then 50 mesh sieves are made into Astragalus powder; corn steep liquor (liquid, moisture content is about 60%) is a by-product of Weifang Shengtai Pharmaceutical Co., Ltd.

[0028] (4) Preparation of seed solution: Lactobacillus rhamnosus was inoculated into a shake flask equipped with seed medium, grown at 37° C. for 18-20 hour...

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Abstract

The invention discloses a method for preparing a feed raw material by using corn steep liquor as a substrate and fermenting astragalus membranaceus with lactobacillus rhamnosus. The method comprises the steps of (1) adding corn steep liquor to astragalus membranaceus powder to prepare a fermenting culture medium, and sterilizing; (2) inoculating lactobacillus rhamnosus strain seed liquid into the fermenting culture medium, then adding sterile water until reaching 30 to 40% of water content in a fermenting system, uniformly mixing, and fermenting in an anaerobic condition; (3) drying the fermenting product to obtain the feed raw material. According to the method, the corn steep liquor that is the industrial byproduct is used for providing a nitrogen source and a carbon source to lactobacillus rhamnosus, so that the rough protein content in the fermenting product can be increased, and the production cost can be increased; in addition, the lactobacillus rhamnosus is used for fermenting astragalus membranaceus, so that the traditional physical chemical method for extracting astragalus polysaccharide and astragaloside can be replaced, and as a result, the contents of astragalus polysaccharide and astragaloside in the fermenting product can be increased, and the problem of environmental pollution caused by the extraction of the physical chemical method can be avoided.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for preparing feed raw materials by fermenting astragalus with Lactobacillus rhamnosus using corn steep liquor as a substrate. Background technique [0002] Corn steep liquor is a by-product of the preparation of corn starch, which is rich in soluble protein, auxin and some precursor substances. Astragalus membranaceus is a leguminous herb, and its dry root is a Chinese herbal medicine resource with great development potential. High food and medicinal value. In 2013, the Ministry of Agriculture listed both corn steep liquor and astragalus in the "Catalogue of Feed Raw Materials", so they have great development prospects in the feed industry. [0003] Astragalus polysaccharides and astragaloside IV are the main active ingredients of Astragalus membranaceus, which have the functions of enhancing cell metabolism, promoting blood circulation, imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/00
CPCY02P60/87
Inventor 林建国王常高杜馨蔡俊
Owner HUBEI UNIV OF TECH
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