Method for treating pesticide residue and heavy metal mercy in sewage

A sewage and pretreatment technology, which is applied in the direction of chemical instruments and methods, filtration treatment, sedimentation treatment, etc., can solve the problems of secondary pollution of soil and water sources, etc., and achieve the effect of improving treatment effect, good application prospect and high removal efficiency

Inactive Publication Date: 2015-09-02
顾博钧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large amount of poisonous gas is released during the process, and a large amount of heat energy is requi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The treatment of embodiment 1 mercury and pesticide residual sewage

[0026] When the sewage enters the water, the concentration of mercury is 30mg / L, dimethoate 20mg / L, fenitrothion 20mg / L, methomyl 20mg / L.

[0027] Pretreatment: remove the suspended solids in the treated sewage by filtration and sedimentation;

[0028] Main treatment: Add the bacteria Pseudomonas putida SPl CGMCC N0.3887 (from CN 102373161A) cultivated to the logarithmic growth phase to the sewage, and flow culture for 24 hours at room temperature to remove the sewage HG;

[0029] Post-treatment: the sewage is treated overnight through the zeolite and activated carbon adsorption pool again to adsorb pesticide residues;

[0030] After the treated sewage is disinfected, it is directly discharged or reused. After testing, the concentration of mercury was 2.2mg / L, dimethoate 15.3mg / L, fenitrothion 14.4mg / L, methomyl 16.5mg / L.

Embodiment 2

[0031] Example 2 Transformation and acquisition of degradable pesticide genes

[0032] According to the method disclosed in CN1970741A, CYP9G2 and POR genes were cloned. The following modifications were made to the CYP9G2 gene. On the basis of GenBank: AB096739, the corresponding Ser30Ile (indicating that the Ser at position 30 was mutated to Ile), Phe56Gly, Gly77Lys, Phe186Ala, Leu276Ile, Leu311Asp, Val366Gly, Val409Cys, Asp415Pro, Trp426Leu, Lys476Gly, Leu500Ala, and the mutation sites were respectively introduced into the protein shown in GenBank: AB096739, thereby constructing different mutant CYP9G2 genes (can be obtained by referring to the preparation method of the prior art). The mutated CYP9G2 gene and the POR gene were respectively connected to the expression vector according to the method for preparing CN1970741A, and the successfully verified recombinant plasmid was transformed into the Pseudomonas putida SP1 CGMCC No. 3887 strain, which was successfully constructe...

Embodiment 3

[0033] Example 3 Verification of sewage degradation effect

[0034] The strains containing different mutant proteins were respectively followed the method of Example 1 to verify the treatment effect on sewage. When the sewage enters the water, the concentration of mercury is 30mg / L, dimethoate 20mg / L, fenitrothion 20mg / L, methomyl 20mg / L. The strains and bacteria transformed with unmutated CYP9G2 and POR genes were used as controls. The specific results are as follows:

[0035] strain Mercury concentration (mg / L) Dimethoate (mg / L) Fenitrothion (mg / L) Methomyl (mg / L) Ser30Ile strain (SC1 strain) 0.1 0.4 0.6 0.6 Phe56Gly strain (SC2 strain) 0.2 0.5 0.5 0.7 Gly77Lys strain (SC3 strain) 0.1 0.4 0.4 0.8 Phe186Ala strain (SC4 strain) 0.2 0.6 0.3 0.7 Leu276Ile strain (SC5 strain) 0.2 0.5 0.5 0.8 Leu311Asp strain (SC6 strain) 0.3 0.4 0.4 0.6 Val366Gly strain (SC7 strain) 0.1 0.3 0.3 0.5 Val409Cys str...

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Abstract

The invention relates to the field of sewage treatment unitization, in particular to a method for reducing pollution of mercury and pesticide residue in sewage. The method can effectively eliminate the pollution of the mercury in the sewage, the method has the advantages of being high in efficiency, low in nutritional requirement, simple in operation and high in safety, compared with existing other technologies, the cost of pollution treatment on the mercury and the pesticide residue in the sewage is obviously reduced, the efficiency of pollution treatment on the mercury and the pesticide residue in the sewage is improved, and the very important practical application value is achieved.

Description

technical field [0001] The invention belongs to the field of sewage treatment, in particular to the treatment of pesticide residues and heavy metal mercury in sewage, and belongs to the technical field of comprehensive sewage treatment. Background technique [0002] In recent decades, chemical pesticides have always been the main means of controlling pests, weeds and rodents in garden agriculture, and their sales have increased year by year. While controlling diseases, pests, weeds and rodents and increasing production, chemical pesticides also destroy the ecology and pollute the environment. 70% of the annual amount of more than 500,000 tons of pesticides will flow into the soil of rivers and rivers. Moreover, some pesticides degrade slowly, and pesticides in the air The concentration will be maintained for quite a long time, and because its residues pollute water areas and food, it directly endangers human health and animal safety, and the polluted surface water directly p...

Claims

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Application Information

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IPC IPC(8): C02F9/04C02F9/14C02F101/20
CPCC02F1/001C02F1/281C02F1/283C02F1/50C02F1/52C02F1/5236C02F1/5245C02F1/56C02F3/34C02F3/342C02F9/00C02F2001/007C02F2101/20C02F2101/306C02F2301/08C02F2303/04
Inventor 朱小明
Owner 顾博钧
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