High-flux quick-detection microfluidic chip directed towards pathogenic microorganism and preparation method for microfluidic chip

Abstract

The invention belongs to the technical field of biomedicine detection analysis, and relates to a high-flux quick-detection microfluidic chip directed towards a plurality of pathogenic microorganism and a preparation method for the microfluidic chip. The high-flux quick-detection microfluidic chip adopts optically transparent polydimethylsiloxane as a material, and is produced by adopting the molding method; a sample reacting liquid micro channel and hemispherical LAMP amplifying micro reaction chambers are formed and arranged in the chip; specific primers directed towards different pathogene microbes are fixed in the micro reaction chambers respectively; parallel analysis directed towards a plurality of microbes for one time can be realized. The high-flux quick-detection microfluidic chip has the characteristics of being high in flux and speed, low in cost, high in portability and simple to produce, and can be used for on-site quick analysis to microbes in medical treatment and health detection work.

Description

technical field [0001] The invention belongs to the technical field of medical and sanitation detection, relates to a microfluidic chip capable of performing high-throughput rapid detection and analysis for multiple pathogenic microorganisms at the same time, and provides a preparation method for the chip. Background technique [0002] Pathogenic microorganisms in public places can cause various diseases to spread and spread among the population. With the continuous acceleration of my country's urbanization process, densely populated urban public places provide conditions for the growth of pathogenic microorganisms. Many infectious diseases have strong concealment and great harm, posing a serious threat to citizens' health. Therefore, the development of new technologies for rapid detection of pathogenic microorganisms has important practical significance for ensuring the safety of public health in our country. For my country's public health monitoring department, it is extr...

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Problems solved by technology

However, this method requires researchers to have extremely rich experience, takes a long time, and the time required for cultivation often ranges from several days to several weeks, and the work efficiency is very low

(2) Immunoassay method, that is, by immunizing higher animals to produce corresponding specific antibodies, and then using the specific recognition reaction of antibodies and antigens to capture the analyte, but the traditional enzyme-linked immunoassay (ELISA) or immunofluorescence method The analysis of (IFA / DFA) takes a long time and is limited by the supply of antibodies, which cannot detect a large number of microorganisms without commercial antibodies

(3) Molecular biology detection methods, including real-time quantitative PCR and DNA microarray technology, etc., all use specific nucleic acid probes, can accurately identify target microorganisms, and have the characteristics of high sensitivity, fast and convenient, and are currently commonly used detection methods, but often rely on large and expensive equipment and professional operators

Therefore, there is a serious problem in the current actual sanitation testing for pathogenic microorganisms: after the samples are collected on site, the results cannot be quickly obtained according to the existing analysis techniques, and they often need to be sent to laboratories in provincial capital cities Analytical testing not only takes days or even weeks, but also is expensive, which is not conducive to the on-site development of health law enforcement

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