Tree Tongue Fermented Extract Feed Additive
A technology for feed and tree tongue, which is applied in the field of fermented extract of tree tongue as feed additive and the production of low-cholesterol livestock and poultry products, can solve the problems that have not yet been seen, achieve the improvement of immune function and intestinal mucosal immune function, and promote animal growth. , Improve the effect of daily weight gain
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Embodiment 1
[0017] The preparation of embodiment 1 tree tongue fermented extract
[0018] (1) Slant culture:
[0019] Quickly inoculate the pure strain of T. japonica into the special slant medium in the ultra-clean bench of the sterile room, and cultivate it for 7-9 days at 26-28°C to obtain the strain of the liquid shake flask; the formula of the special slant medium is: Potato 100 : Glucose 1: Potassium dihydrogen phosphate 1: Agar 1, natural pH;
[0020] Preparation method: Peel 200 g of fresh potatoes and cut into small pieces, add 1000 ml of water and boil for 25 minutes, filter with gauze to get the filtrate, soak the agar in advance, add to the filtrate and boil while stirring continuously until it is completely dissolved, then add glucose and phosphoric acid respectively Potassium dihydrogen and other substances are stirred evenly, dispensed into test tubes while they are hot, corked and placed in a sterilizer for sterilization, steam pressure is 1.1 atmospheres, 120°C, time is ...
Embodiment 2
[0034] The mensuration of embodiment 2 arboricum fluid extract
[0035] This product is tree tongue ( Ganoderma applanatum (Pers.ex Gray.) Pat.) Concentrated extract of fermented liquid and mycelium extract. This product is brown in color, slightly fragrant in gas and slightly bitter in taste.
[0036] Polysaccharide content: Glucose standard curve preparation Accurately weigh 0.1g of anhydrous glucose reference substance dried at 105°C to constant weight, put it in a 100ml volumetric flask, add water to dissolve, dilute to the mark, and shake well to obtain (each 1ml contains water glucose 1mg).
[0037]Preparation of standard curve Precisely measure 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6ml of glucose standard solution, put them in 25ml measuring bottles, add water to 2.0ml, add 3,5-dinitro Salicylic acid test solution 1.5ml, mix well, heat in a boiling water bath for 5 minutes, take it out, immediately cool to room temperature with cold water, add water to dilute to the ...
Embodiment 3
[0044] Example 3 Trial of fermented extract of tree tongue to promote growth of broiler chickens
[0045] 1. Materials and methods
[0046] 1.1 Experimental animals and grouping
[0047] A total of 225 AA broiler chickens with 1-day-old health and similar body weight were selected for the experiment. AA broilers were purchased from Dekun Poultry Food Co., Ltd., Meihekou City, Jilin Province. They were randomly divided into 5 treatment groups, with 3 repetitions in each group and 15 rats in each repetition. The five treatment groups were the control group (corn-soybean meal-based ration), the antibiotic group (basic ration + 5 mg / kg flavomycin) and the GAP group (0.05%, 0.1% and 0.2 % of GAP).
[0048] 1.2 Diet composition and nutrition of experimental animals
[0049] The test feed materials corn, soybean meal and calcium hydrogen phosphate meet the requirements of the national health standard (GB / 13078-2001). The diet was designed with reference to the national broiler ...
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