Submerged fermentation method for ganoderma lucidum

A technology of submerged fermentation and Ganoderma lucidum, applied in the direction of fungi, etc., can solve the problems of high price and difficult acquisition, and achieve the effects of short fermentation cycle, low cost and simple production process

Active Publication Date: 2015-09-09
FUJIAN TIANSIDUN BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The saponin contained in the root of ginseng is its active ingredient. The content of wild ginseng in Changbai Mountain in China is relatively high, but it is not easy to obtain and the price is high.

Method used

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  • Submerged fermentation method for ganoderma lucidum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Strains: strains isolated and selected from wild Ganoderma lucidum tissues in Changbai Mountain, China.

[0031] Submerged fermentation culture method of Ganoderma lucidum and preparation method of dry powder extraction of fermentation product:

[0032] 1. Preparation of slant medium: 1000ml potato juice, 30g skimmed milk powder, 30g glucose, 30g agar, 30g peptone, 5g yeast extract, 0.2g magnesium sulfate, 0.1g vitamin B1, natural pH.

[0033] 2. Slant strain culture: Inoculate high-quality strains on a solid slant medium and culture for 5-7 days at 26-28°C. The mycelium is overgrown on the slant, slightly white, and the strain grows vigorously.

[0034] 3. Erlenmeyer flask shake flask culture: Move the inclined surface strains into the Erlenmeyer flask containing 200ml liquid culture medium, use the shaker at 80-100r / min, stroke 7-8cm reciprocating shaking, 26-28℃ shaking culture for 2-3d, Overgrown with white hyphae, making a seed suspension.

[0035] 4. Prepare the submerged ...

Embodiment 2

[0046] In Example 2, 108.5 kg of dry powder of the active substance of the fermentation product was obtained after measurement and the yield was 36.2 g / L. (The measured moisture content of the dry powder is 5.0%).

Embodiment 3

[0047] In Example 3, 107.8 kg of dry powder of the active substance of the fermentation product was obtained by measurement and the yield was 35.9 g / L. (The measured moisture content of the dry powder is 5.0%).

[0048] Table 1 Main ingredients and content of Ganoderma lucidum fermented dry powder (g / 100g dry weight)

[0049]

[0050] The above-mentioned main component testing standards and methods:

[0051] 1. Polysaccharide (calculated as glucose), phenol-sulfuric acid method.

[0052] 2. Total triterpenes (calculated as ursolic acid), UV spectrophotometric method.

[0053] 3. Protein (calculated on a dry basis, N×6.25), tested with GB5009-2010 standard.

[0054] 4. Amino acids are determined using the method of GB / T 5009.124-2003 automatic amino acid analyzer to determine amino acids in food.

[0055] 5. Soluble dietary fiber is determined by GB / T5009.88 standard method.

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Abstract

The invention discloses a submerged fermentation method for ganoderma lucidum. Natural Chinese herb raw materials such as ginseng, bee pollen, ganoderma lucidum spore powder and indian buead serve as a submerged fermentation culture medium for ganoderma lucidum. The submerged fermentation method has the characteristics of high target product yield, stable quality, good mycelium growth, short fermentation period, simple production process, high extraction yield, rich nutrient, low cost and the like, is an environment-friendly submerged medicinal fungi fermentation technology with remarkable economic benefits, and has a remarkable development and application prospect.

Description

Technical field [0001] The invention belongs to the field of medicinal fungus submerged fermentation production technology and biomedicine, and specifically relates to a method for submerged fermentation of ganoderma lucidum. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is a fungus Ganoderma lucidum (Ganoderma lucidum) belonging to the family Polyporaceae (Polyporaceae) of Ganoderma lucidum (Ganodelma). lucidum·karst and G. lucidum The general name of japonicrnLloyd, which has the functions of strengthening the body and consolidating the body, and is called the top grade by the "Book". In recent years, there has been a lot of research on the chemical constituents and clinical studies of Ganoderma lucidum fungi, and the research on the medicinal value of the spores of Ganoderma lucidum has also received considerable attention. [0003] The spores of Ganoderma lucidum are brown, ovoid, truncated at one end, (8.5~11.2)μm×(5.2~6.9)μm, with double walls. There ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
CPCC12N1/14
Inventor 郭震宇郭耀荣吴松刚
Owner FUJIAN TIANSIDUN BIOTECH
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