Curcumin derivative preparing method

A technology of curcumin derivatives and curcumin, which is applied in the field of preparation of curcumin derivatives, and can solve the problems of poor absorption ability, curcumin's long-lasting efficacy, low selectivity, etc.

Active Publication Date: 2015-09-09
ZHONGRONG TECH CORP LTD
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shortcomings of curcumin in the body, such as not lasting efficacy, low selectivity, poor ability to be absorbe

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Curcumin derivative preparing method
  • Curcumin derivative preparing method
  • Curcumin derivative preparing method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1: Preparation of curcumin conversion products

[0039] (1) Slant culture: Inoculate Rhodococcus ZJPH1003 to the slant medium, cultivate at 30°C for 3 to 4 days to obtain the slant strain; the final concentration of the slant medium is composed of: glucose 10g / L, peptone 5.0g / L , Yeast extract 3.0g / L, (NH 4 ) 2 SO 4 4.0g / L, KH 2 PO 4 1.0g / L, K 2 HPO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, NaCl 0.5g / L, agar 30g / L, solvent is water, pH 7.0, sterilize at 121°C for 20 minutes, cool after sterilization to make a bevel;

[0040] (2) Seed culture: pick a loop of bacteria from step (1) slant bacteria and inoculate it into a 250 mL shake flask containing 100 mL of seed culture medium, cultivate at 30° C. and 200 rpm for 24 hours to prepare seed liquid; the seed culture The final base concentration is composed of: glucose 10g / L, peptone 5.0g / L, yeast extract 3.0g / L, (NH 4 ) 2 SO 4 4.0g / L, KH 2 PO 4 1.0g / L, K 2 HPO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, NaCl 0.5g / L, solvent is water, pH7.0,...

Example

[0066] Example 2-7 The influence of cell concentration on the transformation of Rhodococcus ZJPH1003 to produce product 4

[0067] Using Rhodococcus sp. ZJPH1003 strain, after fermentation and cultivation according to the method of step (4) in Example 1, the wet bacteria were added to a 50mL Erlenmeyer flask containing 10mL 0.1M, pH 6.6 phosphate buffer (wet bacteria The dry weight of the bacteria is 14.4g / L, 21.6g / L, 28.9g / L, 36.1g / L, 43.3g / L, 50.5g / L) (shown in Table 2), the substrate curcumin The final concentration was 50mg / L, and the conversion was performed at 30°C and 200rpm for 36h. After the reaction, the transformation solution was centrifuged to remove the bacterial cells to obtain the supernatant. The supernatant was extracted three times with ethyl acetate. The extracts were combined and concentrated to dryness under reduced pressure at 50°C. After rotary evaporation, the residue obtained was used again with 0.75ml After the chromatographic methanol was re-dissolved...

Example

[0071] Examples 8-14 The influence of the pH value of the transformation solution on the transformation of Rhodococcus ZJPH1003 to produce product 4

[0072] Using Rhodococcus sp. ZJPH1003 strain, after fermentation and cultivation according to the method of step (4) in Example 1, the wet bacteria were added to 10mL 0.1M phosphate buffer solution with pH of 5.4~8.0 respectively (see Table 3 (Shown) in a 50mL Erlenmeyer flask (the final concentration of wet bacteria is 43.3g / L), the substrate curcumin concentration is 50mg / L, and the transformation is carried out at 30°C and 200rpm for 36h. After the reaction, the cells were removed by centrifugation and the supernatant was obtained. The supernatant was extracted three times with ethyl acetate. The extracts were combined and concentrated under reduced pressure to dryness to remove ethyl acetate. After rotary evaporation, the residue obtained was then chromatographed with 0.75ml methanol After re-dissolution, the detection method w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a curcumin derivative preparing method. The curcumin derivative preparing method includes the steps that a conversion reaction is conducted in a phosphate buffer solution with pH value of 5.4-8.0 under the conditions of 25-35 DEG C and 150-250 rpm with wet cells obtained through fermentation cultivation of rhodococcus ZJPH1003 as an enzyme source and curcumin as a substrate, conversion reaction liquid is subjected to postprocessing after the conversion reaction is ended, and curcumin derivatives are obtained. A rhodococcus ZJPH1003 bacterial strain resting cell conversion method is utilized for conducting structural modification on the curcumin, and then the corresponding curcumin derivatives are obtained. The pharmacological or biological activity of the modified curcumin derivatives is improved to different extents compared with the unmodified curcumin substrate, and development of new medicinal preparations is facilitated. The technological process of adopting the microbial conversion method to obtain the curcumin derivatives is simple and environmentally friendly; biocatalyst is made of microbial cells, can be fermented and prepared automatically and has a stable quality and low cost.

Description

(1) Technical field [0001] The invention relates to a preparation method of curcumin derivatives. (2) Background technology [0002] The chemical structural formula of curcumin is: [0003] [0004] Curcumin (curcumin) is a natural active ingredient extracted from the rhizomes of turmeric, curcuma, turmeric, etc., which have anti-cancer, anti-oxidation, anti-inflammatory, anti-HIV, anti-hypertension, anti-cholesterol, Anticoagulation and other biological activities, and its small molecular weight, low toxicity, has good potential for clinical application. However, due to the shortcomings of curcumin in the body, such as not lasting drug effect, low selectivity, poor ability to be absorbed by the body, easy to be degraded, and low bioavailability, its application in the field of health food and medicine is restricted. Therefore, on the basis of retaining the original efficacy of curcumin, the preparation of curcumin derivatives with better performance by chemical or biol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P7/22C12P7/26C12R1/01
CPCC12P7/22C12P7/26
Inventor 王普黄金罗杨春
Owner ZHONGRONG TECH CORP LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap