Dendrobium devonianum rapid propagation seedling survival culture medium series and tissue culture method

Abstract

The invention provides dendrobium devonianum rapid propagation seedling survival culture medium series and a tissue culture method. The dendrobium devonianum rapid propagation seedling survival culture medium series comprise a sowing culture medium, a multiplying culture medium, a seedling strengthening culture medium, a differentiated seedling culture medium and a rooting culture medium. The tissue culture method comprises the steps that seeds are cultivated in a sprouting culture medium firstly, light treatment is directly conducted without darkness cultivation treatment, after the seeds spout and form protocorms, the protocorms are subjected to induction in a protocorms multiplying inducing culture medium to produce callus tissues, and the callus tissues further form protocorm-like bodies; the protocorm-like bodies are subjected to differentiation to form young seedlings; the young seedlings are transplanted into the seedling strengthening culture medium, after the young seedlings grow up to be big seedlings, the big seedlings are cultivated in the rooting culture medium to be dendrobium devonianum mature seedlings. By means of the dendrobium devonianum rapid propagation seedling survival culture medium series and the tissue culture method, the cultivating time of dendrobium devonianum can be shortened, not only is the operation simple, but also the method is not limited by the time and the place, and the continuous division growth of the protocorm-like bodies can guarantee anniversary production, and the large-scale industrial production and application are facilitated.

Description

technical field [0001] The invention relates to a breeding method of medicinal plant seedlings, in particular to a series of mediums for rapid propagation of dendrobium dendrobium and a tissue culture method. Background technique [0002] Dendrobium dendrobii is a perennial herb that likes shade and shade. It likes to grow in warm, humid, semi-shady and semi-sun environment with an annual rainfall of more than 1,000 mm and an average January temperature higher than 8°C. , the suitable growth temperature is 15 to 28 degrees, and the suitable growth air humidity is above 60%. The seeds of Dendrobium dendrobii are as fine as dust, and one capsule contains as many as 1 million seeds. Due to the lack of endosperm, the seeds need to symbiosis with fungi to germinate, and the germination rate is very low (less than 5%) under natural conditions. The natural reproductive capacity of Dendrobium candidum is extremely low, and conventional reproduction is difficult. Over the years, th...

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Problems solved by technology

Due to the lack of endosperm, the seeds need to symbiosis with fungi to germinate, and the germination rate is very low (less than 5%) under natural conditions

[0003] At present, there have been relevant reports on the tissue culture research of Dendrobium dendrobii, but the high cost of tissue culture and the long cycle of planting in test tubes are the bottlenecks restricting the industrialization of Dendrobium dendrobium production

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