Multiplex electrochemiluminescence dna sensor and its application to virus detection
An electrochemistry, virus detection technology, applied in chemiluminescence/bioluminescence, scientific instruments, analysis by chemical reaction of materials, etc., can solve the problem of narrow emission spectrum, wide absorption spectrum, electrochemiluminescence DNA sensor reports Less problems, to achieve the effect of low cost and good sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0047] First, 1.0 μm and 0.05 μm nano-alumina powders (α-Al 2 o 3) Polish the glassy carbon electrode (GCE), and the polished glassy carbon electrode is ultrasonically cleaned with distilled water, ethanol and distilled water to remove the adsorbed substances, and then dried under pure nitrogen environment to obtain a surface-polished glassy carbon electrode; 10 μL of 0.12 mg ·mL -1 Graphene oxide (GO) aqueous solution was dropped on the surface of the glassy carbon electrode and dried at 25 °C to obtain a GO film on the surface of the GCE, which was denoted as GO / GCE; at the potential of 0V to -1.5V, the scan rate was 0.1V s -1 , pH = 7.0 in phosphate buffered saline solution (PBS), under three-electrode mode (glassy carbon electrode as working electrode, platinum wire electrode as counter electrode, saturated calomel electrode as reference electrode), continuously scan the GO film on the surface of GCE After 10 cycles, graphene oxide was electrochemically reduced to obtain...
Embodiment 2
[0052] Firstly, CdTe QDs with an emission wavelength of 551 nm and an aqueous solution of CdTe QDs with an emission wavelength of 551 nm and a CdTe QDs with an emission wavelength of 607 nm were synthesized by a reflux cooling hydrothermal synthesis method using mercaptopropionic acid (MPA) as a stabilizer; Add 0.45mL, 1μmol·L -1 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) aqueous solution and 0.18mL, 1μmol L -1 N-hydroxysulfosuccinimide (Sulfo-NHS) aqueous solution was stirred for 0.5 h to activate the carboxyl groups on the CdTe quantum dots.
[0053] Then, add 100 μL, 10 μmol·L -1 The hepatitis B virus captures DNA; add 100μL, 10μmol·L -1 The hepatitis C virus capture DNA; respectively continue to stir for 3h; through the combination of the carboxyl group on the CdTe quantum dots and the amino group on the virus capture DNA to form a stable amide bond to prepare the hepatitis B virus capture DNA with an emission wavelength of 551nm and an emission wav...
Embodiment 3
[0056] First, reduction of chloroauric acid (HAuC1 4 ) to synthesize gold nanoparticles (AuNPs). Then use 300μL and 1μmol·L respectively -1 Tris-HCl buffer solution to dissolve HBV probe DNA and HCV probe DNA; mix the dissolved HBV probe DNA and HCV probe DNA solutions with 3mL Au NPs solution respectively, and keep it overnight in the dark ; Then add 50 μL, 3mol·L -1 NaCl aqueous solution to adjust the salt concentration to 300mmol L -1 ;
[0057] After standing overnight in the dark, they were centrifuged at 15000rpm for 30min to remove unreacted reagents. Then use 10mmol·L -1 The prepared AuNPs-labeled hepatitis B virus probe DNA and Au NPs-labeled hepatitis C virus probe DNA were washed twice with Tris-HCl (pH=7.4) buffer solution.
[0058] Then the Au NPs-labeled hepatitis B virus probe DNA and Au NPs-labeled hepatitis C virus probe DNA were dissolved in 3 mL of 10 mmol L -1 in Tris-HCl buffer solution. Finally, the Au NPs-labeled hepatitis B virus probe DNA and t...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More