Aqueous enzymatic method for extracting organic rice bran oil
A technology of rice bran oil and water enzymatic method, which is applied in the direction of fat oil/fat production, fat oil/fat refining, fat production, etc. It can solve the problems of high enzymatic hydrolysis temperature, difficulty in breaking emulsion, reducing oil-water interfacial tension, etc., and achieve high extraction rate , The effect of simple process
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Embodiment 1
[0023] (1) Rice bran puffing: glutinous rice bran is used as the raw material, with a fat content of 21.0% and a protein content of 13.5%. After putting the rice bran into the extruder, it is extruded under high pressure and high temperature to form puffed particles;
[0024] (2) Add water to the above rice bran puffed product for extraction, the weight ratio of the rice bran puffed product to water is 1:5, use lactic acid to adjust the pH of the extract to 4.5, add 400 U of cellulase to each gram of rice bran puffed product , acid protease amount 50U, amylase activity 100U, at the same time, add 1.0% CaCl to the rice bran puffed product, stir evenly, enzymolysis temperature 40 ℃, first enzymolysis time 3h, then assist with ultrasonic frequency 30kHz, ultrasonic vibration 20min, filter, The filtrate and filter residue are obtained, and after the filtrate is clarified, the upper crude rice bran oil is extracted for subsequent use. After determination, the residual oil rate of r...
Embodiment 2
[0029] (1) Rice bran puffing: sticky rice (ordinary rice) rice bran is used as raw material, with a fat content of 19.5% and a protein content of 15.5%. After putting the rice bran into the extruder, it is extruded under high pressure and high temperature to form puffed particles;
[0030] (2) Add water to the above rice bran puffed product for extraction, the weight ratio of the rice bran puffed product to water is 1:5, use citric acid to adjust the pH of the extract to 4.5, add the amount of cellulase to each gram of rice bran puffed product 300U, 80U of acid protease, 100U of amylase activity, at the same time add 0.8% CaCl to the puffed rice bran, stir evenly, enzymolysis temperature 40°C, first enzymolysis time 3h, then assist with ultrasonic frequency 30kHz, ultrasonic vibration 20min, filter , to obtain the filtrate and filter residue, and after the filtrate is clarified, the upper part of the crude rice bran oil is extracted for subsequent use. After determination, the...
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