Composite bacterium and application thereof to treatment of garlic processing wastewater

A technology of garlic processing wastewater and compound bacterial agent, which is applied in processing wastewater treatment, food industry wastewater treatment, biological water/sewage treatment, etc., can solve the problem of poor COD degradation ability of water body, increased water treatment cost, and reduced sludge activity, etc. problems, to achieve the effect of strengthening degradation performance and impact resistance, fast response time, and achieving standard discharge

Active Publication Date: 2015-10-28
周俊冉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Decomposed under anaerobic conditions, the organic sulfides rich in garlic processing wastewater produce special odor, which seriously pollutes the environment
[0003] Garlic processing wastewater is generally treated by traditional biochemical methods, but there are inherent disadvantages: 1. Garlic processing wastewater is a very special kind of wastewater, in which allicin is a broad-spectrum fungicide, and it is treated by ordinary activated sludge aeration method , allicin has a strong inhibitory effect on active bacteria, reduces sludge activity, and the degradation ability of COD in water is very poor
2. The COD of garlic processing wastewater is about 2...

Method used

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  • Composite bacterium and application thereof to treatment of garlic processing wastewater
  • Composite bacterium and application thereof to treatment of garlic processing wastewater
  • Composite bacterium and application thereof to treatment of garlic processing wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment one: the preparation of composite bacterial agent

[0024] 1. Strains

[0025] Group A strains:

[0026] Bacillus megaterium: Bacillus megaterium SWSB401

[0027] Pseudomonas stutzeri: Pseudomonas stutzeri SWSP001

[0028] Aspergillus oryzae: Aspergillus oryzae SWSA002

[0029] Klebsiella oxytoca: Klebsiella oxytoca SWSK003

[0030] Desulfovibrio desulfuricans: Desulfovibrio SWSD001

[0031] Group B strains:

[0032] Bacillus megaterium: Bacillus megaterium ATCC12872

[0033] Pseudomonas stutzeri (Pseudomonas stutzeri): Pseudomonas stutzeri ATCC17588

[0034] Aspergillus oryzae: Aspergillus oryzae ATCC 42149

[0035] Klebsiella oxytoca: Klebsiella oxytoca ATCC43086

[0036] Desulfovibrio desulfuricans: Desulfovibrio desulfuricans ATCC 27774

[0037] 2. Medium

[0038] Medium Ⅰ: 1.5g glutathione, 7g beef extract, 1g cysteine, 15g glucose, 2g KCl, (NH 4 ) 3 PO 4 ·3H 2 O 3g, K 2 SO 4 2g, MgCl 2 3g, NaCl 4g, distilled water 1000mL, and autoclave...

Embodiment 2

[0054] Embodiment two: the preparation of composite bacterial agent

[0055] Composite microbial preparation is prepared, and the same part as in Example 1 is not repeated, and the difference is that in step S6, the mixing ratio of each bacterial species component is different, specifically:

[0056] Composite bacterial agent A preparation:

[0057] Step S6, the 5 strains of the strains of group A are divided into 20% of Bacillus megaterium, 10% of Pseudomonas stutzeri, 15% of Aspergillus oryzae, 10% of Klebsiella oxytoca, 10% of desulfurization and desulfurization arc The bacterial cell percentage concentration ratio of 5% bacterial content is mixed, and the composite bacterial agent A of the present invention is obtained.

[0058] Composite bacterial agent B preparation:

[0059] Step S6, the 5 strains of the strains of group B are divided into 20% of Bacillus megaterium, 10% of Pseudomonas stutzeri, 15% of Aspergillus oryzae, 10% of Klebsiella oxytoca, 10% of desulfurizat...

Embodiment 3

[0060] Embodiment three: the preparation of composite bacterial agent

[0061] Composite microbial preparation is prepared, and the same part as in Example 1 is not repeated, and the difference is that in step S6, the mixing ratio of each bacterial species component is different, specifically:

[0062] Composite bacterial agent A preparation:

[0063] Step S6, the 5 strains of the strains of group A are divided into 30% of Bacillus megaterium, 15% of Pseudomonas stutzeri, 35% of Aspergillus oryzae, 35% of Klebsiella oxytoca, 35% of desulfurization and desulfurization arc The bacterial cell percentage concentration ratio of bacterial content of 15% is mixed to obtain the composite microbial agent A of the present invention.

[0064] Composite bacterial agent B preparation:

[0065] Step S6, the 5 strains of the strains of group B are divided into 30% of Bacillus megaterium, 15% of Pseudomonas stutzeri, 35% of Aspergillus oryzae, 35% of Klebsiella oxytoca, 35% of desulfurizati...

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PUM

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Abstract

The invention discloses a composite bacterium, which can be used for the degradation of COD (Chemical Oxygen Demand) and/or organic sulfides and pretreatment for the degradation of garlic processing wastewater. The composite bacterium is obtained by mixing fermentation broth of bacillus megaterium, pseudomonas stutzeri, aspergillus oryzae, klebsiella oxytoca and desulfovibrio desulfuricans, of which bacterial cell concentrations are 20 to 30 percent, 10 to 15 percent, 15 to 35 percent, 10 to 35 percent and 5 to 15 percent respectively. The composite bacterium can be applied to the degradation of COD and/or organic sulfides, and can also be applied to the pretreatment for the degradation of the garlic processing wastewater. By such a biological pretreatment method for the degradation of the garlic processing wastewater, the influence of changes in the conditions of COD and organic sulfide concentrations, water volumes and the like on a degradation effect can be reduced, and use of a plurality of chemical reagents in a subsequent process can be reduced and even avoided; the pretreated wastewater can be discharged up to the standard in an activated sludge treatment system.

Description

technical field [0001] The invention relates to a composite bacterial agent, in particular to a composite bacterial agent that can be applied to COD degradation, organic sulfide degradation, and garlic processing wastewater degradation pretreatment, and belongs to the field of industrial microorganisms. Background technique [0002] Garlic processing wastewater is wastewater discharged from natural garlic processing and production. Garlic processing wastewater is mainly composed of natural organic matter (such as polysaccharides, lipids, etc.), does not contain toxic substances, and has many biodegradable components. Although garlic processing wastewater does not contain toxic substances, it contains a large amount of biodegradable organic substances, and the COD content is about 20000mg / L. If it is directly discharged into the water body without treatment, it will consume a large amount of dissolved oxygen in the water, resulting in the lack of oxygen in the water body and...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C02F3/34C12R1/11C12R1/38C12R1/69C12R1/22C12R1/01C02F101/30C02F103/26
CPCC02F3/34C02F2101/40C02F2103/32C12N1/14C12N1/20
Inventor 周俊冉王曙光韩新玉
Owner 周俊冉
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