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A kind of deproteinized natural rubber and preparation method thereof

A technology of natural rubber and natural rubber latex, which is applied in the field of deproteinized natural rubber and its preparation, can solve problems such as changes, and achieve the effects of improving mechanical precision, avoiding allergies, and good stability

Active Publication Date: 2017-01-18
上海本知医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the serious disadvantages of natural rubber is that the material properties vary depending on the production area and production time, which is a matter of the characteristics of natural products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 compound enzyme preparation

[0040] The compound enzyme preparation used in the present invention comes from the fermentation of Haitobacter sufflavus Tih-w37, A.vinelandii (brown nitrogen-fixing bacteria), B.pumilus (Bacillus pumilus), B.subtilis (Bacillus subtilis), and the strain source and The method of obtaining is as follows:

[0041] ① Haitobacter sufflavus Tih-w37 is a microorganism with independent intellectual property rights, which was preserved in the China Center for Type Culture Collection (CCTCC) by Hao Wei, the inventor of the application, and was selected as a new genus species into the National Natural Science and Technology Resource Platform. Haitobacter sufflavus Tih-w37 was deposited in China Center for Type Culture Collection (abbreviated as CCTCC) on September 1, 2004, deposit number: CCTCC NO.: M204056, deposit location: 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, China No. Wuhan University campu...

Embodiment 2

[0049] Weigh 100 g of ammonia-free concentrated natural rubber latex with a total solid content of 60%, add 0.1 g of sodium dodecyl sulfate (SDS), and then adjust the pH value to 9 with 10% aqueous potassium hydroxide solution to keep the latex stable 140g deionized water was added under stirring, and the total solid content of the reaction system remained at 25%. After fully stirring, after centrifugation at 10000 rpm and 30min by a centrifuge, remove the lower layer of gelatin, leave the upper layer of concentrated gelatin layer, and then add 240g of deionized water under stirring to make the total solid content of the reaction system Keep it at about 25%; then, at 30°C, under the condition of slow stirring, add the compound enzyme preparation prepared in Example 1 (①, ②, ③ and ④ are prepared according to the ratio of Ⅰ) 4:1:0.5:0.5), First, 8 g of the compound preparation of ① and ② were reacted for 5 hours under these conditions. After the reaction finishes, after centrif...

Embodiment 3

[0051] Take by weighing 100g total solids content of 60% ammonia-free concentrated natural rubber latex, add 0.5g sodium dodecyl sulfate (SDS), then adjust the pH value to 9 with 10% aqueous potassium hydroxide solution to keep the latex stable; Subsequently, 140 g of deionized water was added successively, and the total solid content of the reaction system was kept at 25%. After fully stirring, after centrifugation at 10000 rpm and 30min by a centrifuge, remove the lower layer of gelatin, leave the upper layer of concentrated gelatin layer, and then add 240g of deionized water under stirring to make the total solid content of the reaction system Keep it at about 20%; then, at 30°C, under slow stirring conditions, add the compound enzyme preparation prepared in Example 1 (①, ②, ③ and ④ are prepared according to the ratio of Ⅰ) 4:1:0.5:0.5), First, 8 g of ① and ② compound preparations were reacted for 10 hours; then, 2 g of ①, ③ and ④ compound preparations were directly added w...

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Abstract

The invention discloses a deproteinised natural rubber preparation method including the following steps: 1) adding a stabilizer in a fresh natural rubber latex or a concentrated natural rubber latex for stabilizing; 2) adding deionized water to dilute the rubber latex, carrying out centrifugal separation to remove impurities, and collecting an upper rubber phase; 3) diluting the upper rubber phase obtained in the step 2) with deionized water, adding a compound enzyme preparation under stirring and weak alkali conditions, and degrading proteins in the rubber latex under certain reaction conditions; and 4) carrying out centrifugal separation with a centrifugal machine to remove impurities obtained after the proteins are degraded, carrying out repeated degradation to remove the proteins contained in the latex, and thus obtaining deproteinised natural rubber. In addition, the invention also discloses the deproteinised natural rubber prepared by the method. The content of protein amino acids of the deproteinised natural rubber can reach less than a 0.67 ppm reagent detection limit value, and the deproteinised natural rubber can be used in production of various medical latex products and can avoid generation of allergy caused by natural rubber.

Description

technical field [0001] The invention relates to a deproteinized natural rubber and a preparation method thereof, and further relates to a method for avoiding allergy caused by natural rubber. Background technique [0002] Natural rubber is a polymer material naturally synthesized by Hevea brasiliensis. It is mainly composed of cis-polyisoprene and contains more than a dozen components. It has the characteristics of large elongation, high elasticity, film Good strength properties, widely used in industry and agriculture, aerospace, medical and health and other fields. Due to the good biocompatibility and processing operability of natural rubber latex, so far, auxiliary medical devices such as surgical gloves, medical catheters, blood transfusion hoses, examination gloves, and childcare supplies have been widely used in the medical field, and Family planning products, condoms, etc., are mainly produced with concentrated natural rubber latex. [0003] With the widespread use ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08C1/04C12N1/20C12R1/01
Inventor 郝炜郝阳帆乔雅丽
Owner 上海本知医药科技有限公司
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