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Bacillus subtilis with effect of inhibiting clostridium perfringens and application of bacillus subtilis

A technology of Clostridium perfringens and Bacillus subtilis, which is applied in the direction of medical preparations containing active ingredients, bacteria, antibacterial drugs, etc., can solve the problems of large differences in the probiotic performance of Bacillus, and achieve increased daily weight gain , reduce mortality, strong inhibitory effect

Active Publication Date: 2015-11-11
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the probiotic properties of different Bacillus species are quite different, and the screening of new Bacillus subtilis with strong inhibitory effect on Clostridium perfringens is of great significance for the development of new drugs for the prevention and treatment of necrotic enteritis diseases

Method used

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  • Bacillus subtilis with effect of inhibiting clostridium perfringens and application of bacillus subtilis
  • Bacillus subtilis with effect of inhibiting clostridium perfringens and application of bacillus subtilis
  • Bacillus subtilis with effect of inhibiting clostridium perfringens and application of bacillus subtilis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation and identification of Bacillus subtilis with the effect of inhibiting Clostridium perfringens

[0031] 1 Materials and methods

[0032] 1.1 Materials

[0033] 1.1.1 Separation of sources

[0034] Branches, roots, leaves, bark, etc. of Eucommia ulmoides in Tai'an and surrounding areas.

[0035] 1.1.2 Isolation medium

[0036] LB solid medium: 0.5% of yeast extract, 1% of peptone, 1% of NaCl, 1.5% of agar powder, pH value is 7.0; it is calculated by mass percentage.

[0037] 1.1.3 Incline medium

[0038] Separation medium with 1.1.2;

[0039] 1.1.4 Fermentation medium

[0040] The fermentation medium is LB liquid medium, and its composition is: 0.5% of yeast extract, 1% of peptone, 1% of NaCl, and the pH value is 7.0; it is calculated by mass percentage.

[0041] 1.1.5 Medium for indicator bacteria

[0042] Escherichia coli and Staphylococcus aureus are cultured using LB medium, and the medium formula is: 0.5% yeast extract, 1% peptone, 1% NaCl,...

Embodiment 2

[0096] Embodiment 2: the preparation technology of probiotics

[0097] 1 material

[0098] 1.1 Strains: The strain BLCC1-0155 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCCNO: M2015278.

[0099] 1.2 Medium:

[0100] The formula of the seed liquid medium is: glucose 20g / L, peptone 10g / L, yeast extract 5g / L, adjust the pH to 7.0, and sterilize at 115°C for 20 minutes;

[0101] The solid slant medium is the agar powder with a mass fraction of 1.5% added to the above-mentioned seed liquid medium;

[0102] The liquid fermentation medium formula is: glucose 20g / L, peptone 10g / L, yeast extract 5g / L, sodium chloride 5g / L, pH7.0.

[0103] 2 methods

[0104] 2.1 Preparation process of bacteria powder

[0105] (ⅰ) Bacterial species: select Bacillus subtilis BLCC1-0155, CCTCCNO: M2015278;

[0106] (ii) Slant culture: inoculate the lyophilized powder strain of Bacillus subtilis BLCC1-0155 on a solid slant medium, and cultivate at 37...

Embodiment 3

[0114] Embodiment 3: the establishment of chicken necrotic enteritis model

[0115] 1 Materials and methods

[0116] 1.1 Materials

[0117] AA broilers were purchased from Shandong Taian Daewoo Breeding Farm; 510 feed was purchased from Shandong Liuhe Group Co., Ltd. Clostridium perfringens type A CVCC49 was purchased from China Veterinary Microbiology Culture Collection Center.

[0118] 1.2 Method

[0119] Select 50 1-day-old AA broilers and randomly divide them into 5 groups, which are control group (no challenge), challenge 1 group (according to Clostridium perfringens 10 9 The dose of cfu / only to challenge the virus), challenge the poison 2 groups (according to Clostridium perfringens 10 8 The dose of cfu / only to challenge the poison), three groups of the challenge (according to Clostridium perfringens 10 7 The dose of cfu / only to challenge the virus), challenge 4 groups (according to Clostridium perfringens 10 6 The dose of cfu / only to attack poison). The chicks we...

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Abstract

The invention discloses bacillus subtilis with an effect of inhibiting clostridium perfringens; the bacterial strain is named the bacillus subtilis BLCC1-0155. The bacillus subtilis BLCC1-0155 disclosed by the invention has a powerful inhibiting effect on the clostridium perfringens, and also has the inhibiting effect on escherichia coli and staphylococcus aureus. Proved by an animal experiment, a microecologics product taking the bacillus subtilis BLCC1-0155 as an effective component is capable of preventing necrotic enteritis of broilers better, therefore the hurt of the necrotic enteritis to intestinal tracts of the broilers is reduced, and the death rate is greatly reduced; meanwhile, the microecologics product is also capable of increasing the daily gain of the broilers, and is a relatively ideal microecologics product, which can be clinically applied, used for preventing the necrotic enteritis of the broilers.

Description

technical field [0001] The invention relates to a bacillus subtilis capable of inhibiting Clostridium perfringens and its application. Background technique [0002] Necrotic enteritis was discovered and reported in British broilers in 1961, and subsequently reported in most poultry producing countries in the world. The disease has an economically important impact on livestock production worldwide and has been extensively studied to date. In 2011, Zhong Yongqing pointed out in Modern Agricultural Science and Technology that chicken necrotic enteritis is an acute sporadic infectious disease caused by Clostridium welchii type A. Toxins are produced to cause the disease. Clostridium welchii (Clostridium welchii), formerly known as Clostridium perfringengs (CP), was originally isolated by welchii and Nutall from a blood vessel that produced air bubbles in a decayed human corpse, and was named Clostridium welchii. According to the China Poultry Industry Guide, there were only m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A61P31/04A61P1/00C12R1/125
CPCY02A50/30
Inventor 单宝龙陈雷谷巍程福亮徐海燕王红陈甜甜樊梅娜辛国芹聂兆晶房栋赵影王春凤
Owner 山东宝来利来生物工程股份有限公司
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