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Method for preparing silicon hollow nano-spheres by aid of reducing agent which is secretion of microbial cells

A technology of microbial cells and secretions, which is applied in the field of preparing hollow silicon nanospheres, to achieve the effects of good dispersion, good biocompatibility, and simple operation

Inactive Publication Date: 2015-11-11
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial cell secretions can be used as a natural reducing agent due to their rich enzymes. However, most of the related reports focus on the reduction of noble metals. So far, there have been no reports on the preparation of semiconducting non-metallic hollow spheres using microbial cell secretions as reducing agents.

Method used

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  • Method for preparing silicon hollow nano-spheres by aid of reducing agent which is secretion of microbial cells
  • Method for preparing silicon hollow nano-spheres by aid of reducing agent which is secretion of microbial cells

Examples

Experimental program
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Embodiment 1

[0015] Inoculate Saccharomyces cerevisiae colonies in YPD medium and culture for 24 hours, centrifuge to remove cells, and obtain supernatant cell secretion; take 200mL of cell secretion, add 0.0022mol of K 2 SiF 6 , placed in a shaker at a constant temperature of 30° C. for 48 h at a shaking rate of 150 r / min, centrifuged to collect the precipitate at the bottom, washed, and dried to obtain hollow silicon nanospheres. figure 1 It is the XRD collection of illustrative plates of the silicon nanometer hollow sphere that the present embodiment makes, and it can be seen from the figure that the product obtained is consistent with the XRD collection of silicon simple substance, figure 2 It is the HRTEM image of the silicon nano-hollow spheres prepared in this example. It can be seen from the figure that the obtained elemental silicon is a hollow sphere with an average particle diameter of 200 nm.

Embodiment 2

[0017] Inoculate Brevibacterium flavum colonies in YPD medium and culture them for 24 hours, remove the cells by centrifugation, and obtain supernatant cell secretions; take 200 mL of cell secretions, add 0.0022 mol of K 2 SiF 6 , placed in a shaker at 20°C for 60 hours at a constant temperature, with a shaking rate of 100r / min, centrifuged to collect the precipitate at the bottom, washed and dried to obtain hollow silicon nanospheres with an average particle size of 200nm.

Embodiment 3

[0019] Inoculate Escherichia coli colonies in LB medium and cultivate them for 72 hours, remove the cells by centrifugation, and obtain the supernatant cell secretion; take 200 mL of the cell secretion, add 0.0022 mol of Na 2 SiO 3 , placed in a shaker at a constant temperature of 25° C. for 24 hours at a shaking rate of 200 r / min, centrifuged to collect the bottom precipitate, washed, and dried to obtain hollow silicon nanospheres with an average particle size of 200 nm.

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Abstract

The invention discloses a method for preparing silicon hollow nano-spheres by the aid of a reducing agent which is secretion of microbial cells. The method includes steps of (1), inoculating saccharomyces cerevisiae, brevibacterium flavum, escherichia coli, asperigillus flavus, pseudomonas aeruginosa or aspergillus fumigatus in YPD (yeast peptone dextrose) culture media, LB (luria bertani) culture media, CA (cellulose acetate) culture media or potato dextrose agar culture media, cultivating the saccharomyces cerevisiae, the brevibacterium flavum, the escherichia coli, the asperigillus flavus, the pseudomonas aeruginosa or the aspergillus fumigatus in the culture media for 12h-14d and centrifugally removing the cells to obtain secretion of the cells; (2), adding the secretion of the cells into K<2>SiF<6> or Na<2>SiO<3>, shaking the secretion in a shaking bed at the constant temperature of 20-37 DEG C for 16-72h at the shaking speed of 100-300r / min, centrifugally collecting bottom precipitates, and washing and drying the precipitates to obtain the silicon hollow nano-spheres with the average particle sizes of 200nm. The secretion of the cells is a supernatant. The molar concentration of the K<2>SiF<6> or the Na<2>SiO<3> is 0.011mol / L. The method has a series of advantages of environmental friendliness, simplicity in operation, economic efficiency, good biocompatibility and the like.

Description

technical field [0001] The invention belongs to the technical field of synthesis of silicon nanometer materials, and in particular relates to a method for preparing silicon nanometer hollow spheres by using microbial cell secretion liquid as a reducing agent. Background technique [0002] Nanomaterials refer to the strong small size effect, quantum size effect, surface effect and macroscopic quantum tunneling effect due to the geometric size reaching the nanometer scale (1-100nm), in optics, heat, electricity, magnetism, mechanics and chemistry Materials that exhibit many exotic properties are known as new materials in the 21st century. Therefore, the research on the preparation and properties of nanomaterials has become one of the research hotspots at home and abroad in the past 20 years. Silicon is the most abundant element in the earth's crust except oxygen, and it is one of the essential trace elements for the human body. It is also a chemical element necessary for regu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00B82Y40/00C12R1/865C12R1/13C12R1/19C12R1/67C12R1/385C12R1/68
Inventor 马晓明朱郁葱王魁王歌魏子朋杨林
Owner HENAN NORMAL UNIV
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