A kind of production method of antibiotic-free feed
A production method and antibiotic-free feed technology, applied in animal feed, animal feed, food processing, etc., can solve the problems of complicated process, incompatible production process, difficult industrial feed production and promotion, etc., and achieve huge commercial potential, excellent The effect of anti-corrosion performance, major economic benefits and social benefits
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Embodiment 1
[0026] Slant medium: 1.0% glucose, 1.0% corn steep liquor, 2.0% agar, pH 7.5, sterilized at 115°C for 20 min.
[0027] Liquid medium: 2.0% glucose, 1.0% corn steep liquor, 0.1% magnesium sulfate, pH 7.5, sterilized at 115°C for 20 min.
[0028] Streptomyces albus Streptomyces. albulus The spores were inserted into the slant medium and cultured at 30°C for 7 days to obtain the strain slant; then a ring of slant strains was inserted into the liquid medium and cultured at 30°C for 1 day to obtain the microbial culture solution.
[0029] Mix 200 g of corn bran, 180 g of corn germ meal, 100 g of corn steep liquor (dry matter), and 110 g of protein powder. Take 500ml of microbial culture solution and add it to the mixed material. At this time, the water content of the material is greater than 40%. Use 25% ammonia water to adjust the pH of the material to 5.5, and treat it at 35°C for 2 days. The pH is not adjusted during the entire fermentation process, and it naturally decreases ...
Embodiment 2
[0031] 300g soybean meal, 300g DDGS. Take 500 ml of microbial culture solution and add it to the mixed material. At this time, the water content of the material is greater than 40%. It should be noted that the microorganisms added to the material except Streptomyces albicans Streptomyces. albulus , but also add Bulgarian lactic acid bacteria and Candida utilis, both lactic acid bacteria and yeast are commercially available starters. Adjust the pH of the material to 5.5 with 25% ammonia water, and treat it at 35°C for 2 days. The pH is not adjusted during the entire fermentation process, and it naturally decreases with it. The pH is reduced to 5.0 in 12 hours of fermentation, and to 4.5 in 24 hours. At this time, no ε-polylysine can be detected in the material. When fermenting for 36 hours, the pH decreased to 4.2 with 50 mg / kg of ε-polylysine, and at 48 hours, the pH decreased to 3.7 with 100 mg / kg of ε-polylysine and 100 The feed product was obtained after drying at ℃. ...
Embodiment 3
[0033] The process of preparing the fermented feed is the same as in Example 1; it should be noted that the corn bran and germ meal should be crushed to below 80 mesh in advance; The protein feed can be fed to fish.
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